Skip to main content
Log in

Further studies of the ultrastructure of D2O grown winter rye

  • Original Papers
  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

An electron microscopic study of H2O- and D2O-cultured winterrye (Secale cereale L.cv. Winter) seedlings demonstrated that when compared to cells of H2O-cultured plants, cells of D2O-cultured plants contained many ribosomes (both in the cytoplasm and in the plastids), little smooth endoplasmic reticulum and dictyosomes with compressed cisternae and fewer cisternae per dictyosome. Chloroplasts from cells of D2O-cultured plants contained elongate grana with few lamellae per stack and electron dense partitions. Little stroma lamellae was present. However, numerous ribosomes were present in the stroma. Growth in D2O did not appear to affect microtubule morphology or occurrence.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Burgess, J., andD. H. Northcote, 1969: Action of colchicine and heavy water on the polymerization of microtubules in wheat root meristem. Cell Sci.5, 433–454.

    PubMed  Google Scholar 

  • Chorney, W., N. J. Scully, H. L. Crespi, andJ. J. Katz, 1960: The growth of algae in deuterium oxide. Biochim. biophys. Acta37, 280–287.

    PubMed  Google Scholar 

  • Flaumenhaft, E., S. Bose, H. L. Crespi, andJ. J. Katz: 1965: Deuterium isotope effects in cytology. Int. Rev. Cytol.18, 313–361.

    PubMed  Google Scholar 

  • , andJ. J. Katz, 1960 a: Nucleic acids in some deuterated green algae. Science132, 892–894.

    PubMed  Google Scholar 

  • , 1960 b: Cytochemical studies on deuterated green algae. Ann. N. Y. Acad. Sci.84, 634–643.

    Google Scholar 

  • Flickinger, Ch. J., 1971: Alteration in the Golgi apparatus of Amebae in the presence of an inhibitor of protein synthesis. Exp. Cell Res.68, 381–387.

    PubMed  Google Scholar 

  • Inoue, S., H. Sato, andR. W. Tucker, 1963: Heavy water enhancement of mitotic spindle birefringence. Biol. Bull.125, 380–381.

    Google Scholar 

  • Karnovsky, M. J., 1965: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electronmicroscopy. J. Cell Biol.27, 137 A-138 A.

    Google Scholar 

  • Katz, J. J., 1960: Chemical and biological studies with deuterium. Amer. Sci.48, 544–580.

    Google Scholar 

  • ,H. L. Crespi, A. J. Finkel, R. J. Hasterlik, J. F. Thomson, W. Lester Jr., W. Chorney, N. Scully, R. L. Shaffer, andS. H. Sun, 1958: The biology of deuterium. Proc. 2nd Internat. Conf. Peaceful Uses of Atomic Energy25, 173–180.

    Google Scholar 

  • Reynolds, E. S., 1963: The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol.17, 208–212.

    PubMed  Google Scholar 

  • Siegel, B. Z., andA. W. Galston, 1966: Biosynthesis of deuterated isoperoxidases in rye plants grown in D2O. Proc. Nat. Acad. Sci. (U.S.A.)56, 1040–1042.

    Google Scholar 

  • Siegel, S. M., L. A. Halpern, andC. Giumarro, 1964: Germination and seedling growth of winter rye in D2O. Nature201, 1244–1245.

    Google Scholar 

  • Thomson, O. F., 1963: Biological effects of deuterium. Pergamon, New York.

    Google Scholar 

  • Urey, H. C., F. C. Brickweede, andG. M. Murphy, 1932: The hydrogen isotope of mass 2 and its concentration. Phys. Rev.40, 1–15.

    Google Scholar 

  • Waber, J., andW. S. Sakai, 1974: The effect of growth in 99.8% D2O on the ultra-structure of water rye. Plant Physiol.53, 128–130.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Waber, J., Sakai, W.S. Further studies of the ultrastructure of D2O grown winter rye. Protoplasma 84, 273–281 (1975). https://doi.org/10.1007/BF01279357

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01279357

Keywords

Navigation