Abstract
Forty-eight strains of Rhizobium isolated from the root nodules of three species of legumes indigenous to the high tundra (Astragalus alpinus, Oxytropis maydelliana andOxytropis arctobia) are phenotypically heterogenous with respect to intrinsic antibiotic resistance, expression of nitrogenase activityex planta and plasmid content. All of the strains possess a 250–300 kb plasmid and are homologous to each other on the genomic DNA level but have little DNA homology with selected reference strains of well characterized species of rhizobia. The arctic rhizobia have an optimum growth temperature of 23°C and can grow slowly at 5°C. The DNA from four of the isolates, which were selected for detailed investigation, have sequences homologous tonif andnod genes fromRhizobium trifolii.
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References
Beringer JE (1984) R factor transfer inRhizobium leguminosarum. J Gen Microbiol 84:188–198
Canon FC, Riedel GE, Ausubel FM (1979) Overlapping sequences ofKlebsiella pneumoniae nif DNA cloned and characterized. Mol Gen Genet 174:59–66
Cutting JA, Schulman HM (1969) The site of heme synthesis in soybean root nodules. Biochim Biophys Acta 192:486–493
Eckhardt T (1978) A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria. Plasmid 1:584–588
Hodgson ALM, Roberts WP (1983) DNA colony hybridization to identifyRhizobium strains. J Gen Microbiol 129:207–212
Hollis AB, Kloos WE, Elkan GH (1981) DNA: DNA hybridization studies ofRhizobium japonicum and relatedRhizobiaceae. J Gen Microbiol 123:215–222
Jarvis BDW, Dick AG, Greenwood RM (1980) Deoxyribonucleic acid homology among strains ofRhizobium trifolii and related species. Int J Syst Bacteriol 30:42–52
Johnson JL (1981) Genetic characterization. In: Krieg NR (ed) Manual of methods for general bacteriology. American Society of Microbiology, Washington, DC, pp 450–472
Karagatzides JM, Lewis MC, Schulman HM (1985) Nitrogen fixation in the high arctic tundra at Sarcpa Lake, Northwest Territories. Can J Bot 63:974–979
Keyser HH, Bohlool BB, Hu TS, Weber DS (1982) Fast growingRhizobium japonicum from root nodules of soya bean. Science 215:1631–1632
Ligon JM, Scholla MH, Elkan GH (1982) A spectrophotometric DNA-DNA hybridization technique for studying the genetic taxonomy of cowpea rhizobia In: Clark KW, Stephens JHG (eds) Proceedings of the Eighth North American Rhizobium Conference. University of Manitoba Press, Winnipeg, Canada, pp 481–499
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Springer Harbor, NY
Marmur J (1961) A procedure for the isolation of DNA from microorganisms. J Mol Biol 3:208–218
Masterson RV, Russell TR, Atherly AG (1982) Nitrogen fixation (nif) genes and large plasmids ofRhizobium japonicum. J Bacteriol 152:928–931
Masterson RV, Prakash RK, Atherly AG (1985) Conservation of symbiotic nitrogen fixation gene sequences inRhizobium japonicum. J Bacteriol 163:21–26
Pagan JD, Child JJ, Scowcraft WR, Gibson AH (1975) Nitrogen fixation byRhizobium cultured on a defined medium. Nature 256:406–407
Schofield PR, Djordjevic MA, Rolfe BG, Shine J, Watson JM (1983) A molecular linkage map of nitrogenase and nodulation genes inRhizobium trifolii. Mol Gen Genet 192:459–465
Scholla MH, Moorefield JA, Elkan EH (1984) Deoxyribonucleic acid homology between fast-growing soybean-nodulating bacteria and other Rhizobia. Int J Syst Bacteriol 34:283–286
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Vincent JM (1970) A manual for the practical study of the Root-nodule bacteria. Blackwell, Oxford
Vincent JM (1974) Root nodule symbiosis withRhizobium. In: Quispel A (ed) The biology of nitrogen fixation. North-Holland Publishing Co., Amsterdam, pp 265–341
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Caudry-Reznick, S., Prevost, D. & Schulman, H.M. Some properties of arctic rhizobia. Arch. Microbiol. 146, 12–18 (1986). https://doi.org/10.1007/BF00690151
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DOI: https://doi.org/10.1007/BF00690151