Skip to main content
SpringerLink
Log in

Vasoactive intestinal polypeptide-like immunoreactivity and effects of VIP in the swimbladder of the cod,Gadus morhua

  • Published:
Journal of Comparative Physiology B Aims and scope Submit manuscript

Summary

  1. 1.

    Immunohistochemistry has been used to localize the presence of vasoactive intestinal polypeptide (VIP)-like immunoreactivity (IR) in different parts of the swimbladder and in the coeliac and swimbladder arteries of the cod,Gadus morhua. The effects of exogenous porcine VIP on the swimbladder have been studied in a perfused gas gland preparation and on isolated strips from the secretory mucosa (including the muscularis mucosae), the oval edge and the coeliac and swimbladder arteries.

  2. 2.

    VIP-like IR was present in nerve fibres in the muscularis mucosae and submucosa of the swimbladder wall, the oval edge region, the swimbladder artery and the coeliac artery (Figs. 1–5). No VIP-like IR was encountered in the gas gland of the swimbladder. A few IR fibres were seen in the extrinsic nerves supplying the swimbladder (Fig. 6).

  3. 3.

    VIP (10−7 M) in the perfusion fluid increased the flow through the gas gland of the swimbladder perfused in situ (Fig. 8), and isolated strips of the coeliac and swimbladder arteries showed a small decrease in tension in response to prolonged exposure to VIP. VIP also produced a slowly developing, profound decrease in tension of isolated strips of the secretory mucosa and the oval edge of the swimbladder (Fig. 7).

  4. 4.

    It is concluded that a functional innervation by VIP-containing and-releasing nerves may exist in the swimbladder and swimbladder arteries of the cod.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

IR :

immunoreactive, immunoreactivity

VIP :

vasoactive intestinal polypeptide

References

  • Abrahamsson T (1979) Axonal transport of adrenaline, noradrenaline and phenylethanolamine-N-methyl transferase (PNMT) in sympathetic neurons of the cod,Gadus morhua. Acta Physiol Scand 105:316–325

    Google Scholar 

  • Bishop AE, Polak JM, Bloom SR, Pearse AGE (1978) A new universal technique for the immunocytochemical localization of peptidergic innervation. J Endocrinol 77:25–26P

    Google Scholar 

  • Bohr C (1894) The influence of section of the vagus nerve on disengagement of gases in the airbladder of fishes. J Physiol (London) 15:494–500

    Google Scholar 

  • Coons AC (1956) Histochemistry with labelled antibody. Int Rev Cytol 5:1–23

    Google Scholar 

  • Costa M, Buffa R, Furness JB, Solcia E (1980) Immunohistochemical localization of polypeptides in peripheral autonomic nerves using whole mount preparations. Histochemistry 65:157–169

    Google Scholar 

  • Deineka D (1905) Zur Frage über den Bau der Schwimmblase. Z Wiss Zool 78:149–164

    Google Scholar 

  • Dimaline R, Vaillant C, Dockray GJ (1980) The use of region specific antibodies in the characterization and localization of vasoactive intestinal polypeptide-like substances in the rat gastrointestinal tract. Reg Peptides 1:1–16

    Google Scholar 

  • Dockray GJ (1974) Extraction of a secretinlike factor from the intestine of pike (Esox lucius). Gen Comp Endocrinol 23:340–347

    Google Scholar 

  • Dreser H (1892) Notiz über eine Wirkung des Pilokarpins. Arch Exp Pathol Pharmacol 30:159–160

    Google Scholar 

  • Eklund S, Jodal M, Lundgren O, Sjöqvist A (1979) Effects of vasoactive intestinal polypeptide on blood flow, motility and fluid transport in the gastrointestinal tract of the cat. Acta Physiol Scand 105:461–468

    Google Scholar 

  • Fahlén G, Falck B, Rosengren E (1965) Monoamines in the swimbladder ofGadus callarias andSalmo irideus. Acta Physiol Scand 64:119–126

    Google Scholar 

  • Fahrenkrug J, Haglund U, Jodal M, Lundgren O, Olbe L, Schaffalitzky de Muckadell OB (1978) Nervous release of vasoactive intestinal polypeptide in the gastrointestinal tract of cats: possible physiological implications. J Physiol (London) 248:291–305

    Google Scholar 

  • Fänge R (1953) The mechanism of gas transport in the euphysoclist swimbladder. Acta Physiol Scand 30 [Suppl 110]: 1–133

    Google Scholar 

  • Fänge R (1976) Gas exchange in the swimbladder. In: Hughes GM (ed) Respiration in amphibious vertebrates. Academic Press, London New York, pp 189–211

    Google Scholar 

  • Fänge R, Holmgren S (1982) Choline acetyltransferase activity in the fish swimbladder. J Comp Physiol 146:57–61

    Google Scholar 

  • Fänge R, Holmgren S, Nilsson S (1976) Autonomic nerve control of the swimbladder of the goldsinny wrasse,Ctenolabrus rupestris. Acta Physiol Scand 97:292–303

    Google Scholar 

  • Fouchereau-Peron M, Laburthe M, Besson J, Rosselin G, Le Gal Y (1980) Characterization of the vasoactive intestinal polypeptide (VIP) in the gut of fishes. Comp Biochem Physiol 65A:489–492

    Google Scholar 

  • Furness JB, Costa M (1980) Types of nerves in the enteric nervous system. Neuroscience 5:1–20

    Google Scholar 

  • Holmgren S (1983) The effects of putative non-adrenergic, non-cholinergic autonomic transmitters on isolated strips from the stomach of the rainbow trout,Salmo gairdneri. Comp Biochem Physiol 74C:229–238

    Google Scholar 

  • Holmgren S, Nilsson S (1974) Drug effects on isolated artery strips from two teleosts,Gadus morhua andSalmo gairdneri. Acta Physiol Scand 90:431–437

    Google Scholar 

  • Holmgren S, Nilsson S (1983a) VIP-, bombesin- and neurotension-like immunoreactivity in neurons of the gut of the holostean fish,Lepisosteus plathyrhincus. Acta Zool (Stockh) 64:25–32

    Google Scholar 

  • Holmgren S, Nilsson S (1983b) Bombesin-, gastrin/CCK-, 5-hydroxy-tryptamine-, neurotensin-, somatostatin- and VIP-like immunoreactivity and catecholamine fluorescence in the gut of the elasmobranch,Squalus acanthias. Cell Tissue Res 234:595–618

    Google Scholar 

  • Holmgren S, Vaillant C, Dimaline R (1982) VIP-, substance P-, gastrin/CCK-, bombesin-, somatostatin- and glucagon-like immunoreactivities in the gut of the rainbow trout,Salmo gairdneri. Cell Tissue Res 223:141–153

    Google Scholar 

  • Holstein B, Humphrey CS (1980) Stimulation of gastric acid secretion and suppression of VIP-like immunoreactivity by bombesin in the Atlantic codfish,Gadus morhua. Acta Physiol Scand 109:217–233

    Google Scholar 

  • Järhult J, Fahrenkrug J, Hellstrand P, Uddman R (1982) VIP (Vasoactive intestinal polypeptide)-immunoreactive innervation of the portal vein. Cell Tissue Res 221:617–624

    Google Scholar 

  • Langer M, Van Noorden S, Polak JM, Pearse AGE (1979) Peptide hormone-like immunoreactivity in the gastro-intestinal tract and endocrine pancreas of eleven teleost species. Cell Tissue Res 199:493–508

    Google Scholar 

  • Lundberg JM (1981) Evidence for coexistence of vasoactive intestinal polypeptide (VIP) and acetylcholine in neurons of cat exocrine glands. Morphological, biochemical and functional studies. Acta Physiol Scand 112 [Suppl 496]: 1–57

    Google Scholar 

  • McLean JR, Nilsson S (1981) A histochemical study of the gas gland innervation in the Atlantic cod,Gadus morhua. Acta Zool (Stockh) 62:187–194

    Google Scholar 

  • Nilsson S (1971) Adrenergic innervation and drug responses of the oval sphincter in the swimbladder of the cod (Gadus morhua). Acta Physiol Scand 83:446–453

    Google Scholar 

  • Nilsson S (1972) Autonomic vasomotor innervation in the gas gland of the swimbladder of a teleost,Gadus morhua. Comp Gen Pharmacol 3:371–375

    Google Scholar 

  • Nilsson S (1983) Autonomic nerve function in the vertebrates. Springer Verlag, Berlin Heidelberg New York Tokyo

    Google Scholar 

  • Nilsson S, Grove DJ (1974) Adrenergic and cholinergic innervation of the spleen of the cod,Gadus morhua. Eur J Pharmacol 28:135–143

    Google Scholar 

  • Ross LG (1978) The innervation of the resorptive structures in the swimbladder of a physoclist fish,Pollachius virens (L.). Comp Biochem Physiol 61C:385–388

    Google Scholar 

  • Saupe M (1940) Anatomie und Histologie der Schwimmblase des Flußbarsches (Perca fluviatilis) mit besonderer Berücksichtigung des Ovals. Z Zellforsch Mikrosk Anat 30:1–35

    Google Scholar 

  • Schultzberg M, Hökfelt T, Nilsson G, Terenius L, Rehfeld JF, Brown M, Elde R, Goldstein M, Said S (1980) Distribution of peptide- and catecholamine-containing neurons in the gastro-intestinal tract of rat and guinea pig: Immunohistochemical studies with antisera to substance P, vasoactive intestinal polypeptide, enkephalins, somatostatin, gastrin/cholecystokinin, neurotensin and dopamine-β-hydroxylase. Neuroscience 5:689–744

    Google Scholar 

  • Van Noorden S, Patent GJ (1980) Vasoactive intestinal polypeptide-like immunoreactivity in nerves of the pancreatic islet of the teleost fishGillichtys mirabilis. Cell Tissue Res 212:139–146

    Google Scholar 

  • Van Noorden S, Langer M, Polak JM, Pearse AGE (1980) Immunostaining of peptides in the diffuse neuroendocrine system of the gut in some teleost fishes. Gen Comp Endocrinol 40:351

    Google Scholar 

  • Wahlqvist I (1980) Effects of catecholamines on isolated systemic and branchial vascular beds of the cod,Gadus morhua. J Comp Physiol 137:139–143

    Google Scholar 

  • Woodland WNF (1910) Notes on the structure and mode of action of the ‘oval’ in the pollack (Gadus pollachius) and mugil (Mugil chelo). J Mar Biol Assoc U.K. 2:561–565

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Zoophysiology, University of Göteborg, Box 25059, S-40031, Göteborg, Sweden

    Kersti Lundin & Susanne Holmgren

Authors
  1. Kersti Lundin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Susanne Holmgren
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lundin, K., Holmgren, S. Vasoactive intestinal polypeptide-like immunoreactivity and effects of VIP in the swimbladder of the cod,Gadus morhua . J Comp Physiol B 154, 627–633 (1984). https://doi.org/10.1007/BF00684417

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00684417

Keywords

Search

Navigation