Abstract
In higher plants the only obligate organoauxotrophic mutants available are concerned with the synthesis of thiamine, vitamin B1. In Arabidopsis 65 mutants, representing four loci controlling four different steps in the thiamine pathway, have been obtained and identified. Heteroalleles were distinguished within two loci by qualitative and quantitative responses to low and high temperatures. The availability of auxotrophs in higher plants makes it possible to employ highly selective genetic techniques in well-differentiated diploid organisms.
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This work was supported by National Science Foundation Grant GB 6577 and U.S. Atomic Energy Commission Contract AT-(11-1)-1609. This is a contribution from the Missouri Agricultural Experiment Station. Journal Series Number 5477. Approved by the Director.
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Li, S.L., Rédei, G.P. Thiamine mutants of the crucifer, Arabidopsis . Biochem Genet 3, 163–170 (1969). https://doi.org/10.1007/BF00520351
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DOI: https://doi.org/10.1007/BF00520351