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Adrenergic innervation of the human testis, epididymis, ductus deferens and prostate: a fluorescence microscopic and fluorimetric study

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Summary

  1. 1.

    The localization of catecholamines and 5-hydroxytryptamine has been investigated in the human testis, epididymis, ductus deferens and prostate obtained at operations on patients aged 26–79 years.

  2. 2.

    In fluorimetric determinations noradrenaline was found to be present in varying concentrations in the genital tract. The lowest amount (0.07 μg/g) was found in the testis. In proximal direction along the ejaculatory duct system there was a progressive increase in the noradrenaline level, reaching a maximum of 1.43 μg/g in ductus deferens. The prostatic tissue (obtained from hyperplastic glands) contained only 0.15 μg/g noradrenaline.

    No dopamine was present. Adrenaline occurred, by all probability as an artefact, only in tissues from one single case to which this amine had been administered as part of the local anaesthetic mixture given before operation.

  3. 3.

    Fluorescence microscopic analysis of formaldehyde-treated genital organs revealed the presence of adrenergic nerves, the number of which well agreed with the noradrenaline concentrations.

    Only vascular nerves were found in the testis. The bulk of the adrenergic nerve terminals in the epididymis, ductus deferens and prostate innervated the smooth muscle coats, whereas only a smaller portion of the adrenergic nerve population was of vasomotor nature.

  4. 4.

    Microspectrophotometric characterization of the formaldehyde-induced fluorescence in the tissue sections confirmed that the adrenergic nerves contained noradrenaline.

  5. 5.

    The finding of fluorescent ganglion cells in the prostate indicated that part — if not all — of the male genital tract also in humans is innervated by way of so-called short adrenergic neurons, originating in peripherally located sympathetic ganglia.

  6. 6.

    Flask-shaped cells, morphologically resembling the enterochromaffin cells and emitting a yellow indole fluorescence, were present in the epithelium of the prostatic mucosa.

  7. 7.

    Apart from the specific, formaldehyde-induced monoamine fluorescence, a considerable amount of autofluorescence of varying colour and related to different structures occurred both in the epithelial layers and in the connective tissue.

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References

  • Baumgarten, H. G.: Über die Verteilung von Catecholaminen im Darm des Menschen. Z. Zellforsch. 83, 133–146 (1967).

    Google Scholar 

  • -, A. F. Holstein: Fluoreszierende Sphaeridien in Zellkernen des menschlichen Nebenhodenepithels. To be published (1968).

  • Bertler, A., A. Carlsson, E. Rosengren and B. Waldeck: A method for the fluorimetric determination of adrenaline, noradrenaline and dopamine in tissues. Kgl. Fysiogr. Sällsk. Lund Förh. 28, 121–123 (1958).

    Google Scholar 

  • Björklund, A., B. Ehinger, and B. Falck: A method for differentiating dopamine from noradrenaline by microspectrofluorometry. J. Histochem. Cytochem. (1968) (in press).

  • Corrodi, H., N.-A. Hillarp, and G. Jonsson: Fluorescence methods for the histochemical demonstration of monoamines. 3. Sodium borohydride reduction of the fluorescent compounds as a specificity test. J. Histochem. Cytchem. 12, 582–586 (1964).

    Google Scholar 

  • —, and G. Jonsson: The formaldehyde fluorescence method for the histochemical demonstration of biogenic monoamines. J. Histochem. Cytochem. 15, 65–78 (1967).

    Google Scholar 

  • Euler, U. S. v.: An adrenaline like action in extracts from the prostate and related glands. J. Physiol. (Lond.) 81, 102–112 (1934).

    Google Scholar 

  • —: Noradrenaline. Springfield (Ill): Charles C. Thomas Publ. Co. 1956.

    Google Scholar 

  • —: Neurotransmission in the adrenergic nervous system. Harvey Lect. Ser. 55, 43–65 (1961).

    Google Scholar 

  • —, and F. Lishajko: A specific kind of noradrenaline granules in the vesicular gland and the vas deferens of the bull. Life Sci. 5, 687–691 (1966).

    Google Scholar 

  • Falck, B.: Observations on the possibilities of the cellular localization of monoamines by a fluorescence method. Acta physiol. scand. 56, Suppl. 197, 1–25 (1962).

    Google Scholar 

  • —, J. Häggendal, and Ch. Owman: The localization of adrenaline in adrenergic nerves in the frog. Quart. J. exp. Physiol. 48, 253–257 (1963).

    Google Scholar 

  • —, N. A. Hillarp, G. Thieme, and A. Torp: Fluorescence of catecholamines and related compounds condensed with formaldehyde. J. Histochem. Cytochem. 10, 348–354 (1962).

    Google Scholar 

  • —, and Ch. Owman: A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines. Acta Univ. Lund. II, 7, 1–23 (1965).

    Google Scholar 

  • — —, and N. O. Sjöstrand: Peripherally located adrenergic neurons innervating the vas deferens and the seminal vesicle of the guinea-pig. Experientia (Basel) 21, 98–100 (1965).

    Google Scholar 

  • Forsberg, J.-G., E. Rosengren, and N. O. Sjöberg: On the occurrence of 5-hydroxytryptamine containing cells in the vaginal and vestibular epithelium of the rabbit. Z. Zellforsch. 63, 302–308 (1964).

    Google Scholar 

  • Gomori, G.: Observations with differential stains on human islets of Langerhans. Amer. J. Path. 17, 395–406 (1941).

    Google Scholar 

  • Häggendal, J.: An improved method for fluorimetric determination of small amounts of adrenaline and noradrenaline in plasma and tissues. Acta physiol. scand. 59, 242–254 (1963).

    Google Scholar 

  • Hamberger, B., T. Malmfors, and Ch. Sachs: Standardization of paraformaldehyde and of certain procedures for the histochemical demonstration of catecholamines. J. Histochem. Cytochem. 13, 147 (1965).

    Google Scholar 

  • Holstein, A. F.: Die glatte Muskulatur in der Tunica albuginea des Hodens und ihr Einfluß auf den Spermatozoentransport in den Nebenhoden. Anat. Anz., Erg.-Bd. 121, 103–108 (1968).

    Google Scholar 

  • —: Morphologische Studien am Nebenhoden des Menschen. In: Zwanglose Abhandlungen aus dem Gebiet der normalen und pathologischen Anatomie, hrsg. v. W. Bargmann u. W. Doerr. Stuttgart: Georg Thieme 1968 (im Druck).

    Google Scholar 

  • Jacobowitz, D., and G. B. Koelle: Histochemical correlations of AcChE and catecholamines in postganglionic autonomic nerves of the cat, rabbit and guinea-pig. J. Pharmacol. exp. Ther. 148, 225–237 (1965).

    Google Scholar 

  • Jarvik, M. E.: Drugs used in the treatment of psychiatric disorders. I. The phenothiazine derivatives. In: Goodman and Gilman, The Pharmacological Basis of Therapeutics, p. 159–178. New York: Macmillan & Co. 1965.

    Google Scholar 

  • Langley, J. N., and H. K. Anderson: The innervation of the pelvic and adjoining viscera. J. Physiol. (Lond.) 19, 71–139 (1895).

    Google Scholar 

  • Leidl, W.: Vergleichende andrologische Betrachtungen aus dem Gebiete der Veterinär- und Humanmedizin. In: Neue Ergebnisse der Andrologie, S. 1–7. Berlin-Heidelberg-New York: Springer 1965.

    Google Scholar 

  • Nickerson, M.: Drugs inhibiting adrenergic nerves and structures innervated by them. In: Goodman and Gilman, The Pharmacological Basis of Therapeutics, p. 546–572. New York: Macmillan & Co. 1965.

    Google Scholar 

  • Norberg, K. A.: Transmitter histochemistry of the sympathetic adrenergic nervous system. Brain Res. 5, 125–170 (1967).

    Google Scholar 

  • —, P. L. Risley, and U. Ungerstedt: Adrenergic innervation of the male reproductive ducts in some mammals. Z. Zellforsch. 76, 278–286 (1967).

    Google Scholar 

  • Owman, Ch., E. Rosengren, and N. O. Sjöberg: Adrenergic innervation of the human female reproductive organs: A histochemical and chemical investigation. Obstet. Gynec. 30, 763–773 (1967).

    Google Scholar 

  • —, and N. O. Sjöberg: Difference in rate of depletion and recovery of noradrenaline in „short“ and „long“ sympathetic nerves after reserpine treatment. Life Sci. 6, 2549–2556 (1967).

    Google Scholar 

  • —, and N. O. Sjöstrand: Short adrenergic neurons and catecholamine-containing cells in vas deferens and accessory male genital glands of different mammals. Z. Zellforsch. 66, 300–320 (1965).

    Google Scholar 

  • — —: On short adrenergic neurons in the accessory male genital organs of the bull. Experientia (Basel) 22, 759–761 (1966).

    Google Scholar 

  • Ritzén, M.: Cytochemical identification and quantitation of biogenic monoamines. M. D. Thesis Stockholm 1967. Stockholm: Norstedt & Söner 1967.

    Google Scholar 

  • Romeis, B.: Mikroskopische Technik. München: Oldenbourg 1968.

    Google Scholar 

  • Sjöstrand, N. O.: Effect of reserpine and hypogastric denervation on the noradrenaline content of the vas deferens and seminal vesicle of the guinea-pig. Acta physiol. scand. 56, 376–380 (1962).

    Google Scholar 

  • —: The adrenergic innervation of the vas deferens and the accessory male genital glands. Acta physiol. scand. 65, Suppl. 257, 1–82 (1965).

    Google Scholar 

  • —, and G. Swedin: Effect of reserpine on the noradrenaline content of the vas deferens and seminal vesicle compared with the submaxillary gland and the heart of the rat. Acta physiol. scand. 72, 370–371 (1968).

    Google Scholar 

  • Stjärne, L., and F. Lishajko: Comparison of spontaneous loss of catecholamines and ATP in vitro from isolated bovine adrenomedullary, vesicular gland, vas deferens and splenic nerve granules. J. Neurochem. 13, 1213–1216 (1966).

    Google Scholar 

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Authors and Affiliations

  1. Abteilung für Neuroanatomie, Anatomisches Institut der Universität Hamburg, Deutschland

    H. G. Baumgarten, B. Falck, A. -F. Holstein, Ch. Owman & T. Owman

  2. Institute of Anatomy and Histology, University of Lund, Sweden

    H. G. Baumgarten, B. Falck, A. -F. Holstein, Ch. Owman & T. Owman

Authors
  1. H. G. Baumgarten
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  2. B. Falck
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  3. A. -F. Holstein
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  4. Ch. Owman
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  5. T. Owman
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Additional information

Supported by grant No. B68-12X-732-03B from the Swedish Medical Research Council, by the Deutsche Forschungsgemeinschaft (Ba 310/1; Fl, 26/12), and by Ford Foundation, New York.

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Baumgarten, H.G., Falck, B., Holstein, A.F. et al. Adrenergic innervation of the human testis, epididymis, ductus deferens and prostate: a fluorescence microscopic and fluorimetric study. Z. Zellforsch 90, 81–95 (1968). https://doi.org/10.1007/BF00496704

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  • DOI: https://doi.org/10.1007/BF00496704

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