Abstract
The tissue distribution of deoxyribonucleases has been studied in the organs of Drosophila hydei at the onset of metamorphosis. The enzymes were separated by disc electrophoresis and detected directly in the gel. An extensive shift in the wide spectrum of activities that has been observed at metamorphosis indicates that deoxyribonucleases play an important role at this time in development. On the basis of the tissue distribution of these enzymes, it has been possible to assign probable functions to several of the activities. An intense activity appears in the prepupal salivary gland which is not detected in this organ in the larval stage. This observation is of particular interest in view of the changes that have been observed in the chromosomal puffing patterns of the salivary glands just prior to metamorphosis. A class of activities, which is probably of lysosomal origin, is more prevalent in the prepupal tissues. The data suggest that an increased synthesis of lysosomes is a general reaction of most larval tissues at the onset of metamorphosis irrespective of whether a tissue undergoes total histolysis. The larval intestine contains a factor which strongly inhibits Drosophila nucleases that are active at low pH. The major nuclease activities of each tissue have been tentatively characterized. A knowledge of the enzyme properties is expected to facilitate the isolation of DNA from the individual tissues.
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References
Beermann, W. (1966). Differentiation at the level of the chromosomes. In Cell Differentiation and Morphogenesis, North Holland Publishing Co., Amsterdam, p. 24.
Berendes, H. D. (1965). Salivary gland function and chromosomal puffing patterns in Drosophila hydei. Chromosoma 17 35.
Berendes, H. D. (1966). Gene activities in the Malpighian tubules of Drosophila hydei at different developmental stages. J. Exptl. Zool. 162 209.
Bernardi, G., and Griffe, M. (1964). Studies on acid deoxyribonuclease. II. Isolation and characterization of spleen-acid deoxyribonuclease. Biochemistry 3 1419.
Boyd, J. B. (1969). Drosophila deoxyribonucleases. I. Variation of deoxyribonucleases in Drosophila melanogaster. Biochim. Biophys. Acta 171 103.
Boyd, J. B., and Mitchell, H. K. (1965). Identification of deoxyribonucleases in polyacrylamide gel following their separation by disk electrophoresis. Anal. Biochem. 13 28.
Boyd, J. B., Berendes, H. D., and Boyd, H. (1968). Mass preparation of nuclei from the larval salivary glands of Drosophila hydei. J. Cell Biol. 38 369.
Kilby, B. A. (1963). The biochemistry of the insect fat body. Advan. Insect Physiol. 1 112.
Laufer, H., and Nakase, Y. (1965). Developmental studies of the dipteran salivary gland. II. DNase activity in Chironomus thummi. J. Cell Biol. 25 97.
Locke, M., and Collins, J. V. (1967). Protein uptake in multivesicular bodies in the molt-intermolt cycle of an insect. Science 155 467.
Lockshin, R. A., and Williams, C. M. (1965a). Programmed cell death—V. Cytolytic enzymes in relation to the breakdown of the intersegmental muscles of silkmoths. J. Insect Physiol. 11 831.
Lockshin, R. A., and Williams, C. M. (1965b). Programmed cell death—I. Cytology of degeneration in the intersegmental muscles of the Pernyi silkmoth. J. Insect Physiol. 11 123.
Mukai, J., and Soeta, E. (1967). Action of silkworm endonuclease on oligoribonucleotides terminating in 3′-phosphate. Biochim. Biophys. Acta 138 1.
Schin, K. S., and Clever, U. (1965). Lysosomal and free phosphatase in salivary glands of Chironomus tentans. Science 150 1053.
Schin, K. S., and Clever, U. (1968). Ferritin-uptake by salivary glands of Chironomus tentans and its intracellular localization. Exptl. Cell Res. 49 208.
Stay, B. (1965). Protein uptake in the oocytes of the Cecropia moth. J. Cell Biol. 26 49.
Wessing, A. (1962). Elektronenmikroskopische Studien zur Funktion der Malpighischen Gefaesse von Drosophila melanogaster. I. Die Fefaesse der Larve und Imago. Protoplasma 55 264.
Wessing, A. (1963). Elektronenmikroskopische Studien zur Funktion der Malpighischen Gefaesse von Drosophila melanogaster. II. Die Gefaesse der Puppe. Protoplasma 56 433.
Whitten, J. M. (1964). Haemocytes and the metamorphosing tissues in Sarcophago bullata, Drosophila melanogaster, and other cyclonhaphous diptera. J. Insect Physiol. 10 477.
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This work was performed in the Max-Planck-Institut für Biologie, Abteilung Beermann, Tübingen, Germany. The senior author was supported by the Helen Hay Whitney Foundation.
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Boyd, J.B., Boyd, H. Deoxyribonucleases of the organs of Drosophila hydei at the onset of metamorphosis. Biochem Genet 4, 447–459 (1970). https://doi.org/10.1007/BF00486595
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DOI: https://doi.org/10.1007/BF00486595