Abstract
Sulphate uptake by Anacystic nidulans under aerobic conditions in the light was found to be sensitive to metabolic poisons, such as N, N′-dicyclohexyl-carbodiimide and carbonyl cyanide m-chlorophenyl hydrazone. It was also depressed by darkness. The sulphate absorption is an energy-dependent process. Sulphate uptake was also inhibited by chromate and selenate.
Osmotic shock strongly affected sulphate uptake. This effect could be interpreted by a loss of a binding protein involved in the absorption of sulphate. Osmotic shock also depressed oxygen production in light and oxygen consumption in darkness; however, shocked cells were able to grow normally.
Sulphate uptake was strongly enhanced by sulphate starvation, but this enhancement was partly prevented by chloramphenicol. Apparently sulphate starvation depressed the synthesis of a carrier involved in the transport of sulphate. During sulphate starvation the membrane potential, measured by the uptake of triphenylmethylphosphonium, increases. This may be due to changes in the membrane.
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Abbreviations
- ATP:
-
adenosine 5′-triphosphate
- CCCP:
-
carbonyl cyanide m-chlorophenyl hydrazone
- DCCD:
-
N,N′-dicyclohexylcarbodiimide
- DCMU:
-
3-(3,4-dichlorophenyl)1,1-dimethylurea
- EDTA:
-
ethylene-diamine tetraacetic acid
- HEPES:
-
N-2-hydroxyethylpiperazine-N′-2-ethane sulfonic acid
- TPMP+ :
-
triphenylmethylphosphonium
- TRIS:
-
2-amino-2-hydroxylmethyl-1,3-propandiol
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Jeanjean, R., Broda, E. Dependence of sulphate uptake by Anacystis nidulans on energy, on osmotic shock and on sulphate starvation. Arch. Microbiol. 114, 19–23 (1977). https://doi.org/10.1007/BF00429625
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DOI: https://doi.org/10.1007/BF00429625