Abstract
The anti-auxin 4-chlorophenoxyisobutyric acid (PCIB) applied at a concentration of 10-2 mol m-3 to maize root segments was found to induce a transmembrane electrical potential of up to-130 mV (Δpd of 30 mV). The kinetics of this response were comparable to the time scale for PCIB-stimulated H+-extrusion. Both effects are eliminated by the addition of p-fluoromethoxycarbonyl cyanide phenylhydrazone (FCCP). Treatment with fusicoccin (FC) and PCIB together does not result in a hyperpolarization greater than with FC alone. Benzoic acid (10-2 mol m-3) had no effect on the transmembrane electrical potentials. These results are discussed in relation to a possible electrogenic proton pump which may be regulated by perturbations in the cellular auxin content or activity.
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Abbreviations
- ATPase:
-
adenosine triphosphatase
- FC:
-
fusicoccin
- FCCP:
-
p-fluoromethoxy carbonylcyanide phenylhydrazone
- IAA:
-
indole-3yl-acetic acid
- NAA:
-
naphthyl-lylacetic acid
- PCIB:
-
4-chlorophenoxyisobutyric acid
- PD:
-
potential difference
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Moloney, M.M., Pilet, PE., Marrè, MT. et al. Transmembrane electrical potentials in growing maize roots. Planta 156, 407–412 (1982). https://doi.org/10.1007/BF00393311
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DOI: https://doi.org/10.1007/BF00393311