Summary
The frequencies with which the generalized transducing phage P1 transduced 26 selected markers on the E. coli. chromosome were measured. The frequencies were found to vary relative to argH +=1 from a maximum of 6.8 near the origin of replication to a minimum of 0.23 for a marker not far from the terminus. The low frequencies obtained for some markers were shown not to result from poor expression under the selective conditions employed. When plotted as a function of marker position on the chromosome the frequencies were found to exhibit a series of peaks and troughs which correspond to those in gene density noted by Bachmann et al. (1976). The possible relationship of these results to the structure of the E. coli chromosome and to the mechanism of generalized transduction are discussed.
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Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K12. Bact. Rev. 40, 116–166 (1976)
Bird, R.E., Louarn, J., Martuscelli, J., Caro, L.: Origin and sequence of chromosome replication in Escherichia coli. J. molec. Biol. 70, 549–566 (1972)
Caro, L., Berg, C.M.: Chromosome replication in Escherichia coli II. Origin of replication of F- and F+ strains. J. molec. Biol. 45, 325–336 (1969)
Chelala, C.A., Margolin, P.: Effects of deletions on cotransduction linkage in Salmonella typhimurium: evidence that bacterial chromosome deletions affect the formation of transducing DNA fragments. Molec. gen. Genet. 131, 97–112 (1974)
Cooper, S., Helmstetter, C.E.: Chromosome replication and the division cycle of Escherichia coli B/r. J. molec. Biol. 31, 519–540 (1968)
Essenberg, R.C., Kornberg, H.L.: Location of the gene specifying hexose phosphate transport (uhp) on the chromosome of Escherichia coli. J. gen. Microbiol. 99, 157–169 (1977)
Harriman, P.D.: A single-burst analysis of the production of P1 infectious and transducing particles. Virology 48, 595–600 (1972)
Hiraga, S.: Novel F prime factors able to replicate in Escherichia coli Hfr strains. Proc. nat. Acad. Sci. (Wash.) 73, 198–202 (1976)
Hohlfeld, R., Vielmetter, W.: Bidirectional growth of the E. coli chromosome. Nature (Lond.) New biol. 242, 130–132 (1973)
Kadner, R.J., Winkler, H.: Isolation and characterization of mutations affecting the transport of hexose phosphates in Escherichia coli. J. Bact. 113, 895–900 (1973)
Lennox, E.S.: Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1, 190 (1955)
Masters, M.: Origin and direction of replication of the chromosome of E. coli B/r. Proc. nat. Acad. Sci. (Wash.) 65, 601–608 (1970)
Masters, M.: Strains of Escherichia coli diploid for the chromosomal origin of DNA replication. Molec. gen. Genet. 143, 105–111 (1975)
Masters, M., Broda, P.: Evidence for the bidirectional replication of the Escherichia coli chromosome. Nature (Lond.) New Biol. 232, 137–140 (1971)
Miller, J.H.: Experiments in Molecular Genetics. 1 st edit., pp. 230–234. New York: Cold Spring Harbor Laboratory 1972
Parker, D.L., Glaser, D.A.: Chromosomal sites of DNA-membrane attachment in Escherichia coli. J. molec. Biol. 87, 153–168 (1974)
Pittard, J.: Effect of integrated sex factor on transduction of chromosomal genes in Escherichia coli. J. Bact. 89, 680–686 (1965)
Prasad, I., Schaefler, S.: Regulation of the β-glucoside system in Escherichia coli K12. J. Bact. 120, 638–650 (1974)
Sandri, R.M., Berger, H.: Bacteriophage P1 mediated generalized transduction in Escherichia coli: Molecular mechanisms; in: Abstracts of the Annual Meeting of the American Society for Microbiology, p. 136. Washington: American Society for Microbiology 1977
Schaefler, S., Maas, W.K.: Inducible system for the utilization of β-glucosides in Escherichia coli II. Description of mutant types and genetic analysis. J. Bact. 93, 264–272 (1967)
Wolf, B., Newman, A., Glaser, D.A.: On the origin and direction of replication of the Escherichia coli K12 chromosome. J. molec. Biol. 32, 611–629 (1968)
Worcel, A., Burgi, E.: On the structure of the folded chromosome of Escherichia coli. J. molec. Biol. 71, 127–147 (1972)
Yudkin, M.D.: Mutations in Escherichia coli that relieve catabolite repression of tryptophanase synthesis. Mutations distant from the tryptophanase gene. J. gen. Microbiol. 92, 125–132 (1976)
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Masters, M. The frequency of P1 transduction of the genes of Escherichia coli as a function of chromosomal position: Preferential transduction of the origin of replication. Molec. gen. Genet. 155, 197–202 (1977). https://doi.org/10.1007/BF00393160
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DOI: https://doi.org/10.1007/BF00393160