Abstract
When two types of mammalian cells were treated with ethidium bromide for several hours, the mitotic figures showed no chromatid breaks or exchanges but a high incidence of sticky chromosomes. Electron microscopic examinations revealed that many chromosomes are connected by submicroscopic chromatin strands of various widths. Chromosome stickiness, therefore, is interpreted as entanglement of chromatin fibers between unrelated chromosomes, probably caused by abnormal condensation behaviors prior to mitosis. Presumably, chromatin breaks would occur when sticky chromosomes separate during anaphase. Such microscopically undetectable breaks expressed as various kinds of chromosomal aberrations in the next mitosis when the damaged cells were permitted to recover in the absence of ethidium bromide.
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McGill, M., Pathak, S. & Hsu, T.C. Effects of ethidium bromide on mitosis and chromosomes: a possible material basis for chromosome stickiness. Chromosoma 47, 157–166 (1974). https://doi.org/10.1007/BF00331803
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DOI: https://doi.org/10.1007/BF00331803