Abstract
By means of radioautography the synthesis of RNA and of proteins during the spermatocyte divisions of Pales ferruginea was investigated. The RNA synthesis decreases from early to late prophase and is blocked during and after the divisions. Protein synthesis is at a high level in prophase, and continues during the division even until the end of spermiohistogenesis. Chloramphenicol depresses protein synthesis and delays the beginning of meiotic divisions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literatur
Baserga, R.: A study of nucleic acid synthesis in ascites tumor cells by two emulsion autoradiography. J. Cell Biol. 12, 633–637 (1962).
Bibring, T., Cousineau, G. H.: Percentage incorporation of Leucine labeled with 14C into isolated mitotic apparatuses during early development of sea urchin eggs. Nature (Lond.) 204, 805–807 (1964).
Bier, K., Kunz, W., Ribbert, D.: Struktur und Funktion der Oocytenchromosomen und Nukleolen sowie der Extra-DNS während der Oogenese panoistischer und meroistischer Insekten. Chromosoma (Berl.) 23, 214–254 (1967).
Buck, C. A., Granger, G. A., Holland, J. J.: Initiation and completion of mitosis in HeLa cells in the absence of protein synthesis. Currents Mod. Biol. 1, 9–15 (1967).
Byfield, J. E., Scherbaum, O. H.: Incomplete inhibition of synchronized cell division by hydroxyurea and its relevance to the normal cellular life cycle. Nature (Lond.) 216, 1017–1018 (1967).
—: Temperature effect on protein synthesis in a heat-synchronized protozoan treated with Actinomycin D. Science 156, 1504–1505 (1967).
—: Temperature-dependent decay of RNA and of protein synthesis in a heatsynchronized protozoan. Proc. nat. Acad. Sci. (Wash.) 57, 602–606 (1967).
Cummins, J. E., Rusch, H. P.: Limited DNA-synthesis in the absence of protein synthesis in Physarum polycephalum. J. Cell. Biol. 31, 577–585 (1966).
Dietz, R.: Bau und Funktion des Spindelapparates. Naturwissenschaften 56, 237–248 (1969).
Errera, M., Brunfaut, M.: Observations of mitotic figures in pulse labeled HeLa cells. Exp. Cell Res. 33, 105–111 (1964).
Feinendegen, L. E., Bond, V. P.: Observations on nuclear RNA during mitosis in human cancer cells in culture (HeLa-S3), studied with tritiated cytidine. Exp. Cell Res. 30, 393–404 (1963).
Henderson, S. A.: RNA synthesis during male meiosis and spermatogenesis. Chromosoma (Berl.) 15, 345–366 (1964).
Hotta, Y., Stern, H.: Synthesis of messenger-like ribonucleic acid and protein during meiosis in isolated cells of Trillium erectum. J. Cell Biol. 19, 45–58 (1963).
— Parchman, L. G., Stern, H.: Protein synthesis during meiosis. Proc. nat. Acad. Sci. (Wash.) 60, 572–582 (1968).
Jacobson, B. S., Salmon, R. J., Lansky, L. L.: Antimitotic effects of Chloramphenicol and other inhibitory agents in Chlamydomonas. Exp. Cell Res. 36, 1–13 (1964).
Jockusch, B.: Protein synthesis during the first three cleavages in pond snail eggs (Lymnaea stagnalis), Z. Naturforschg. 23b, 1512–1516 (1968).
Konrad, C. G.: Protein synthesis and RNA synthesis during mitosis in animal cells. J. Cell Biol. 19, 267–277 (1963).
Lazarus, L. H., Levy, M. R., Scherbaum, O. H.: Inhibition of synchronized cell division in Tetrahymena by Actinomycin D. Exp. Cell Res. 36, 672–676 (1964).
Mangan, J., Miki-Noumura, T., Gross, R.: Protein synthesis and the mitotic apparatus. Science 147, 1575–1578 (1965).
Mittermayer, M., Braun, T., Rusch, H. P.: The effect of Actinomycin D on the timing of mitosis in Physarum polycephalum. Exp. Cell Res. 38, 33–41 (1965).
Parchman, L. G., Stern, H.: The inhibition of protein synthesis in meiotic cells and its effect on chromosome behavior. Chromosoma (Berl.) 26, 298–311 (1969).
Petzelt, Ch., Bier, K.: Hemmung und Induktion von Proteinsynthesen durch Actinomycin D in den wachsenden Oocyten von Musca domestica. Wilhelm Roux' Archiv (im Druck).
Prescott, D. M., Bender, M. A.: Synthesis of RNA and protein during mitosis in mammalian tissue culture cells. Exp. Cell Res. 26, 260–268 (1962).
Salb, J. M., Marcus, P. I.: Translational inhibition in mitotic HeLa cells. Proc. nat. Acad. Sci. (Wash.) 54, 1353–1358 (1965).
Scharff, M. D., Robbins, E.: Polyribosome disaggregation during metaphase. Science 151, 992–995 (1966).
Sisken, J. E., Wilkes, E.: The time of synthesis and the conservation of mitosis-related proteins in cultured human amnion cells. J. Cell Biol. 34, 97–110 (1967).
— Iwasaki, T.: The effects of some amino acid analogs on mitosis and the cell cycle. Exp. Cell Res. 55, 161–167 (1969).
Sofer, W. H., George, J. F., Iverson, R. M.: Tate of protein synthesis: Regulation during first division cycle of sea urchin eggs. Science 153, 1644–1645 (1966).
Stafford, D. W., Iverson, R. M.: Radioautographic evidence for the incorporation of 14C-Leucine into the mitotic apparatus. Science 143, 580–581 (1964).
Taylor, J. H.: Autoradiographic studies of nucleic acid and proteins during meiosis in Lilium longiflorum. Amer. J. Bot. 46, 477–484 (1959).
—: Nucleic acid synthesis in relation to the cell division cycle. Ann. N.Y. Acad. Sci. 90, 409–421 (1960).
Went, H. A.: Studies of the mitotic apparatus of the sea urchin by means of antigen-antibody reactions in agar. J. biophys. biochem. Cytol. 6, 447–455 (1959).
Wilt, F. H., Sakai, H., Mazia, D.: Old and new protein in the formation of the mitotic apparatus in cleaving sea urchin eggs. J. mol. Biol. 27, 1–7 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Petzelt, C. RNS- und Proteinsynthese im Ablauf der Spermatocytenteilungen von Pales ferruginea (Nematocera) . Chromosoma 29, 237–245 (1970). https://doi.org/10.1007/BF00326081
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00326081