Summary
The location of the characters for rifampicin-resistance in E. coli linked to the arg E marker was confirmed by mating experiments with rifampicin-resistant Hfr C mutants and a rifampicin-sensitive acceptor strain. During these tests, temporary dominance of rifampicin-sensitivity was observed. On the basis of the results obtained, the time required for complete phenotypic expression of rifampicin-resistance was determined, and the ratios of rifampicin-sensitivity to rifampicin-resistance in the merozygote phase of the cell were studied. It was found that the episomal marker of rifampicin-sensitivity influences the level of resistance in merodiploid cells, but does not fully restore the original sensitivity of the wild type.
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Communicated by W. Arber
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Knüsel, F., Schiess, B. Dominance study with rifampicin-resistant mutants of E. coli K12. Molec. Gen. Genet. 108, 331–337 (1970). https://doi.org/10.1007/BF00267770
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DOI: https://doi.org/10.1007/BF00267770