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Gonadotropic cells in the Atlantic salmon, Salmo salar

An experimental immunocytological, electron microscopical study

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Summary

The gonadotropin-producing cells (GTH-cells) in the Atlantic salmon were studied light and electron microscopically before, during and after spawning, and after injections of luteinizing hormone-releasing hormone (LH-RH). The double immunofluorescent technique was applied using rabbit anti-carp GTH as the first antibody. Numerous immunofluorescent cells were observed throughout the pars distalis, but very few in the pars intermedia. These cells are basophilic and PAS-positive, and ultrastructurally classified as globular gonadotropes. Only one gonadotropic cell type could be identified; its size, morphology and fine structure vary considerably. In the same specimen the GTH-cells can be predominantly globular or vesicular in appearance, depending on the reproductive phase of the fish. At spawning and after LH-RH injection, many GTH-cells reach a vacuolar stage; the content of the vacuoles is not immunofluorescent. Another cell type, which resembles GTH-cells in semithin sections, did not show gonadotropic properties; its nature and functional significance are unknown. In addition, the present study revealed an increase in the synthetic and exocytotic activity of prolactin cells after LH-RH injections. It is suggested that LH-RH mediates this effect via LH and eventually via estradiol.

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Authors and Affiliations

  1. Department of Zoology, University of Stockholm, Stockholm, Sweden

    Bo Ekengren & Gunnar Fridberg

  2. Zoological Laboratory, Section Comparative Endocrinology, State University of Utrecht, Utrecht, The Netherlands

    Jan Peute

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  1. Bo Ekengren
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  2. Jan Peute
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  3. Gunnar Fridberg
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The authors are indebted to Dr. N. Johansson and superintendent N. Steffner for generously providing facilities at their institutes in Älvkarleby. They also wish to thank Mrs. Y. Lilliemarck and Mrs. V. Lundin for their skillful technical assistance. The grants made available by the Swedish Natural Science Research Council (No. 2124-037) are also gratefully acknowledged. Thanks are also due to Mrs. S.M. McNab for linguistic assistance

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Ekengren, B., Peute, J. & Fridberg, G. Gonadotropic cells in the Atlantic salmon, Salmo salar . Cell Tissue Res. 191, 187–203 (1978). https://doi.org/10.1007/BF00222419

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