No interspecific hybrids between C. amoena ssp. huntiana and C. lassenensis were found in a naturally occurring sympatric population. The few F1-diploid hybrids produced experimentally showed a wide range as well as a high degree of meiotic irregularities which are probably the main factors in determining hybrid sterility. In most meiocytes only univalents were present at M1. The majority of M1 associations encountered were non-homologous pseudo-associations, the rest were chiasmate-like and some of these could be homoeologous. Meiotic abnormalities resulted, in part, from such pseudo-associations together with precocious division of univalents and fragmentation prior to A1. Pollen tetrad analysis revealed higher numbers of microspores produced from a single hybrid PMC, apparently as a result of microsporocyte supernumerary divisions. These microspores varied in size and number of nuclei and were virtually all sterile. The magnitude of F1 cytological abnormalities and the extremely well developed reproductive isolation between these two otherwise relatively close species, indicate a karyotype diversity that could happen only if we assume extensive chromosomal rearrangements resulting in profound differences between their respective genomes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdel-Hameed, F. (1967). Cytogenetic and evolutionary studies of the tetraploid Clarkia gracilis and its diploid ancestors. Thesis, University of California, Davis.
Abdel-Hameed, F. (1970). Reproductive and developmental differentiation within section Primigenia of the genus Clarkia. Trans. Ill. Acad. Sci. 63: 347–358.
Abdel-Hameed, F. (1971). Cytogenetic studies in Clarkia, section Primigenia. V. Interspecific hybridization between C. amoena huntiana and C. lassenensis. Evolution 25: (347–355).
Abdel-Hameed, F. & R. Snow (1968). Cytogenetic studies in Clarkia, section Primigenia. IV. A Cytological survey of Clarkia gracilis. Amer. J. Bot. 55: 1047–1054.
Beadle, G. W. (1929). A gene for supernumerary mitoses during spore development in Zea mays. Science 70: 406–407.
Beadle, G. W. (1931). A gene in maize for supernumerary cell divisions following meiosis. Cornell Univ. Agr. Expt. Sta. Mem. 135: 1–12.
Koul, A. K. (1970). Supernumerary cell divisions following meiosis in the spider plant. Genetica 41: 305–310.
Levan, A. (1942). Studies on the meiotic mechanism of haploid rye. Hereditas 28: 177–211.
Lewis, H. (1953). The mechanism of evolution in the genus Clarkia. Evolution 7: 1–20.
Lewis, H. & M. E. Lewis (1955). The genus Clarkia. Univ. of Calif. Publ. Bot. 20: 241–392.
Lewis, H. & P. H. Raven (1958). Rapid evolution in Clarkia. Evolution 12: 319–336.
Margulis, L. (1970). Origin of Eukaryotic Cells. Yale University Press, New Haven.
Margulis, L. (1971). Whittaker's five kingdoms of organisms: minor revisions suggested by considerations of the origin of mitosis. Evolution 25: 242–245.
Mc Clintock, B. (1933). The association of non-homologous parts of chromosomes in mid-prophase of Zea mays. Z. Zellforsch. mikr. Anat. 19: 191–237.
Mooring, J. S. (1958). A Cytogenetic study of Clarkia unguiculata. I. Translocations. Amer. J. Bot. 45: 233–242.
Muller, H. J. (1940). Analysis of the process of structural change in chromosomes of Drosophila. J. Genet. 40: 1–66.
Östergren, G. & E. Vigfusson (1953). On position correlation of univalents and quasi-bivalents formed by sticky univalents. Hereditas 39: 3–50.
Rieger, R. (1957). Inhomologenpaarung und Meioseablauf bei haploiden Formen von Antirrhinum majus L. Chromosoma 9: 1–38.
Snow, R. (1960). Chromosomal differentiation in Clarkia dudleyana. Amer. J. Bot. 47: 302–309.
Snow, R. (1963a). Cytogenetic studies in Clarkia, section Primigenia. I. A Cytological survey of Clarkia amoena. Amer. J. Bot. 50: 337–348.
Snow, R. (1963b). Alcoholic hydrochloric acid-carmine as a stain for chromosomes in squash preparations. Stain Technol. 38: 9–13.
Snow, R. & A. Imam (1964). Cytogenetic studies in Clarkia, section Primigenia. II. A Cytological survey of Clarkia arcuata and Clarkia lassenensis. Amer. J. Bot. 51: 160–165.
Stebbins, G. L. (1950). Variation and evolution in plants. Columbia Univ. Press, New York.
Sybenga, J. (1965). The quantitative analysis of chromosome pairing and chiasma formation based on the relative frequencies of MI configurations. III. Telocentric trisomics. Genetica 36: 351–361.
Sybenga, J. (1966a). The sygomere as hypothetical unit of chromosome pairing initiation. Genetica 37: 186–198.
Sybenga, J. (1966b). The quantitative analysis of chromosome pairing and chiasma formation based on the relative frequencies of MI configurations. V. Interchange trisomics. Genetica 37: 481–510.
Vasek, F. C. (1958). The relationship of Clarkia exilis to Clarkia unguiculata. Amer. J. Bot. 45: 270–275.
Walters, M. S. (1954). A study of pseudo-bivalents in meiosis of two interspecific hybrids of Bromus. Amer. J. Bot. 41: 160–171.
Walters, M. S. (1957). Studies of spontaneous chromosome breakage in interspecific hybrids of Bromus. Univ. Calif. Publ. Bot. 28: 335–447.
Author information
Authors and Affiliations
Additional information
Scientific journal series paper 409, contribution from the Department of Biological Sciences, Northern Illinois University.
Rights and permissions
About this article
Cite this article
Abdel-Hameed, F. Chromosomal abnormalities in F1-diploid hybrids between Clarkia lassenensis and C. Amoena ssp. Huntiana . Genetica 43, 3–24 (1972). https://doi.org/10.1007/BF00122496
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00122496