Abstract
Interpretation of photosynthetic pigment data using iterative programs such as CHEMTAX are widely used to examine algal community structure in the surface ocean. The accuracy of such programs relies on understanding the effects of environmental parameters on the pigment composition of taxonomically diverse algal groups. Phaeocystis antarctica is an important contributor to total autotrophic production and the biogeochemical cycling of carbon and sulfur in the Southern Ocean. Here we report the results of a laboratory culture experiment in which we examined the effects of ambient dissolved iron concentration on the pigment composition of colonial P. antarctica, using a new P. antarctica strain isolated from the southern Ross Sea in December 2003. Low-iron (<0.2 nM dissolved Fe) filtered Ross Sea seawater was used to prepare the growth media, thus allowing sub-nanomolar iron additions without the use of EDTA to control dissolved iron concentrations. The experiment was conducted at relatively low irradiance (~20 μE m−2 s−1), with P. antarctica primarily present in the colonial form—conditions that are typical of the southern Ross Sea during austral spring. Relative to the iron-limited control treatments (0.22 nM dissolved Fe), iron addition mediated a decrease in the ratio of 19′-hexanoyloxyfucoxanthin to chlorophyll a, and an increase in the ratio of fucoxanthin to chlorophyll a. Our results also suggest that the ratio of 19′-hexanoyloxyfucoxanthin to chlorophyll c3 (Hex:Chl c3 ratio) may be a characteristic physiological indicator for the iron-nutritional status of colonial P. antarctica, with higher Hex:Chl c3 ratios (>3) indicative of Fe stress. We also observed that the ratio of fucoxanthin to 19′-hexanoyloxyfucoxanthin (Fuco:Hex ratio) was highly correlated (r 2 = 0.82) with initial dissolved Fe concentration, with Fuco:Hex ratios <0.05 measured under iron-limited conditions (dissolved Fe <0.45 nM). Our results corroborate and extend the results of previous experimental studies, and, combined with pigment measurements from the southern Ross Sea, are consistent with the hypothesis that the interconversion of fucoxanthin and 19′-hexanoyloxyfucoxanthin by colonial P. antarctica is used as a photo-protective or light-harvesting mechanism, according to the availability of dissolved iron.
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References
Arrigo KR, Weiss AM, Smith WO (1998) Physical forcing of phytoplankton dynamics in the southwestern Ross Sea. J Geophys Res 103(C1):1007–1022
Arrigo KR, Robinson DH, Worthen DL, Dunbar RB, DiTullio GR, van Woert M, Lizotte MP (1999) Phytoplankton community structure and the drawdown of nutrients and CO2 in the Southern Ocean. Science 283:365–367
Arrigo KR (2005) Marine microorganisms and global nutrient cycles. Nature 437(7057):349–355
Bertrand EM, Saito M, Rose JM, Riesselman C, Lohan MC, Noble AE, Lee P, DiTullio GR (in press) Vitamin B12 and iron co-limitation of phytoplankton growth in the Ross Sea. Limnol Oceanogr
Buma AG, Bano JN, Veldhuis MJW, Kraay GW (1991) Comparison of the pigmentation of two strains of the prymnesiophyte Phaeocystis sp. Neth J Sea Res 27:173–182
Coale KH, Wang X, Tanner SJ, Johnson KS (2003) Phytoplankton growth and biological response to iron and zinc addition in the Ross Sea and Antarctic circumpolar current along 170°W. Deep-Sea Res II 50:635–653
Coale KH, Gordon RM, Wang X (2005) The distribution and behavior of dissolved and particulate iron and zinc in the Ross Sea and Antarctic circumpolar current along 170°W. Deep-Sea Res I 52:295–318
Crocker KM, Ondrusek ME, Petty RL, Smith RC (1995) Dimethylsulfide, algal pigments and light in an Antarctic Phaeocystis sp. bloom. Mar Biol 124:335–340
DiTullio GR, Hutchins DA, Bruland KW (1993) Interaction of iron and major nutrients controls phytoplankton growth and species composition in the tropical North Pacific Ocean. Limnol Oceanogr 38:495–508
DiTullio GR, Smith WO Jr (1995) Relationship between dimethylsulfide and phytoplankton pigment concentrations in the Ross Sea, Antarctica. Deep-Sea Res I 42:873–892
DiTullio GR, Smith WO Jr (1996) Spatial patterns in phytoplankton biomass and pigment distributions in the Ross Sea. J Geophys Res 101:18467–18477
DiTullio GR, Grebmeier JM, Arrigo KR, Lizotte MP, Robinson DH, Leventer AR, Barry JP, van Woert M, Dunbar RB (2000) Rapid and early export of Phaeocystis antarctica blooms in the Ross Sea, Antarctica. Nature 404:595–598
DiTullio GR, Geesey ME (2002) Photosynthetic pigments in marine algae and bacteria. In: G Bitton (ed) The Encyclopedia of environmental microbiology. John Wiley & Sons, Inc, New York, NY, pp 2453–2470
DiTullio GR, Geesey ME, Jones DR, Daly KL, Campbell L, Smith WO Jr (2003a) Phytoplankton assemblage structure and primary productivity along 170o W in the South Pacific Ocean. Mar Ecol Prog Ser 255:55–80
DiTullio GR, Geesey ME, Leventer AR, Lizotte MP (2003b) Algal pigment ratios in the Ross Sea: Implications for CHEMTAX analysis of Southern Ocean Data. In: DiTullio GR, Dunbar RB (eds) Biogeochemistry of the Ross Sea. AGU Antarctic Research Series 78, Washington, DC, pp 35–52
El-Sayed SZ, Biggs D, Holm-Hansen O (1983) Phytoplankton standing crop, primary productivity, and near-surface nitrogenous nutrient fields in the Ross Sea, Antarctica. Deep-Sea Res I 30:871–886
Fitzwater SE, Johnson KS, Gordon RM, Coale KH, Smith WO (2000) Trace metal concentrations in the Ross Sea and their relationship with nutrients and growth. Deep-Sea Res II 47:3159–3179
Gerringa LJA, de Baar HJW, Timmermans KR (2000) A comparison of iron limitation of phytoplankton in natural oceanic waters and laboratory media conditioned with EDTA. Mar Chem 68:335–346
Gibson JA, Garrick ERC, Burton HR, McTaggart AR (1990) Dimethylsulfide and the alga Phaeocystis pouchetti in Antarctic coastal waters. Mar Biol 104:339–346
Greene RM, Geider RJ, Kolber Z, Falkowski PG (1992) Iron-induced changes in light harvesting and photochemical energy conversion processes in eukaryotic marine algae. Plant Physiol 100(2):565–575
Guillard RRL, Hargraves PE (1993) Stichochrysis immobilis is a diatom, not a chrysophyte. Phycologia 32: 234–236
Mackey MD, Mackey DJ, Higgins HW, Wright SW (1996) CHEMTAX—a program for estimating class abundances from chemical markers: application to HPLC measurements of phytoplankton. Mar Ecol Prog Ser 144:265–283
Martin JH, Fitzwater SE, Gordon RM (1990) Iron deficiency limits phytoplankton growth in Antarctic waters. Global Biogeochem Cycles 4:5–12. Research Series, 78:209–220, Washington, DC
Morel FMM, Hudson RJM, Price NM (1991) Limitation of productivity by trace metals in the sea. Limnol Oceanogr 36:1742–1755
Palmisano AC, SooHoo JB, SooHoo SL, Kottmeier ST, Craft LL, Sullivan CW (1986) Photoadaptation in Phaeocystis pouchetii advected beneath annual sea ice in McMurdo Sound, Antarctica. J Plankton Res 8:891–906
Raven JA (1990) Predictions of Mn and Fe use efficiencies of phototrophic growth as a function of light availability for growth and C assimilation pathway. New Phytol 116:1–17
Rousseau V, Vaulot D, Casotti R, Cariou V, Lenz J, Gunkel J, Baumann M (1994) The life cycle of Phaeocystis (Prymnesiophyceae): evidence and hypotheses. J Mar Syst 5(1):23–39
Schoemann V, Becquevort S, Stefels J, Rousseau V, Lancelot C (2005) Phaeocystis blooms in the global ocean and their controlling mechanisms: a review. J Sea Res 53:43–66
Sedwick PN, DiTullio GR, Mackey DJ (2000) Iron and manganese in the Ross Sea, Antarctica: seasonal iron limitation in Antarctic shelf waters. J Geophys Res 105(C5):11, 321–11, 336
Sedwick PN, Garcia NS, Riseman SF, DiTullio GR (2006) Evidence for high iron requirements for colonial Phaeocystis antarctica at low irradiance. Biogeochemistry (this issue)
Smith WO, Marra J, Hiscock MR, Barber RT (2000) The seasonal cycle of phytoplankton biomass and primary productivity in the Ross Sea, Antarctica. Deep Sea Res II 47:3119–3140
Smith WO Jr, Dennett MR, Mathot S, Caron DA (2003) The temporal dynamics of the flagellated and colonial stages of Phaeocystis antarctica in the Ross Sea. Deep Sea Res II 50:605–617
Smith WO Jr, Van Hilst CM (2003) Effects of assemblage composition on the temporal dynamics of carbon and nitrogen uptake. In: DiTullio GR, Dunbar RB (eds) Biogeochemistry of the Ross Sea, American Geophysical Union, Washington DC, pp197–208
Sunda WG, Huntsman SA (1997) Interrelated influence of iron, light and cell size on marine phytoplankton growth. Nature 390:389–392
Trull TS, Rintoul R, Hadfield M, Abraham ER (2001) Circulation and seasonal evolution of polar waters south of Australia: implications for iron fertilization of the Southern Ocean. Deep-Sea Res II 48:2439–2466
Van Leeuwe MA, Stefels J (1998) Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae). II. Pigment composition. J Phycol 34:496–503
Wright SW, Jeffrey SW, Mantoura RFC, Llewellyn CA, Bjørnland T, Repeta D, Welschmeyer NA (1991) Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Mar Ecol Prog Ser 77:183–196
Zapata M, Rodriguez F, Garrido JL (2000) Separation of chlorophylls and carotenoids from marine phytoplankton:a new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases. Mar Ecol Prog Ser 195:29–45
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DiTullio, G.R., Garcia, N., Riseman, S.F., Sedwick, P.N. (2007). Effects of iron concentration on pigment composition in Phaeocystis antarctica grown at low irradiance. In: van Leeuwe, M.A., Stefels, J., Belviso, S., Lancelot, C., Verity, P.G., Gieskes, W.W.C. (eds) Phaeocystis, major link in the biogeochemical cycling of climate-relevant elements. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6214-8_7
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