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Characterization of harpinXoo induced hypersensitive responses in non host plant, tobacco

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Abstract

Harpin proteins encoded by hrp genes are bacterial protein elicitors that can stimulate hypersensitive response (HR) in non-host plants. HR-related pathogen resistance involves a complex form of programmed cell death (PCD). It is increasingly viewed as a key component of the hypersensitive disease response of plants. Currently, the evidence of harpin proteins-induced PCD is deficient though it exhibits phenotypic parallels with HR, and the mechanism of harpin proteins-induced PCD is not well understood. In this study, we demonstrate that harpinXoo protein from Xanthomonas oryzae pv. oryzae of rice bacterial blight expressed and isolated from bacterial cells acted as an agent to induce PCD in infiltrated tobacco plants. Treatment of tobacco leaves with harpinXoo induced typical PCD-related morphological and biochemical changes including cell shrinkage and nuclear DNA degradation. We further analyzed the expression of several genes in signal transduction pathway of PCD in tobacco plants by real-time qRT-PCR analysis using EF-1α as an endogenous control. Our results showed that the expression of NtDAD1 was down-regulated and the expression of BI-1, tpa1 and aox1 was up-regulated following the infiltration of harpinXoo into tobacco leaves. Our data suggest that harpinXoo can induce PCD with the coordination of PCD-related genes in infiltrated tobacco leaves, providing evidence to further investigate the signal transduction pathways of HR and PCD.

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Abbreviations

PCD:

Programmed cell death

HR:

Hypersensitive response

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Acknowledgments

This work was supported by Grants from National Natural Science Foundation of China (31160359, 31360029), Doctoral Fund of Ministry of Education (20124601110004, 20104601110004) and Research and start-up capital of Hainan University (kyqd1006).

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    Authors and Affiliations

    1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource/College of Environment and Plant Protection, Hainan University, Haikou, 570228, China

      Linlin Xie, Yue Liu, Hui Wang, Wenbo Liu, Rong Di, Weiguo Miao & Fucong Zheng

    2. Department of Plant Biology, Rutgers, the State University of New Brunswich, New Brunswich, NJ, 08901, USA

      Rong Di

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    1. Linlin Xie
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    2. Yue Liu
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    3. Hui Wang
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    4. Wenbo Liu
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    6. Weiguo Miao
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    Correspondence to Weiguo Miao.

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    We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled, “HarpinXoo-induced hypersensitive response shares the characteristics of programmed cell death and alters the expression of programmed cell death-related genes in tobacco”.

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    Linlin Xie and Yue Liu contributed equally to this work and are regarded as joint first authors.

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    Xie, L., Liu, Y., Wang, H. et al. Characterization of harpinXoo induced hypersensitive responses in non host plant, tobacco. J. Plant Biochem. Biotechnol. 26, 73–79 (2017). https://doi.org/10.1007/s13562-016-0363-9

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