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Indole-3-acetic acid reverses the harpin-induced hypersensitive response and alters the expression of hypersensitive-response-related genes in tobacco

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Abstract

Harpin proteins stimulate hypersensitive response (HR) in plants. However, the mechanism by which HR is regulated is not clear. The role of the auxin, indole-3-acetic acid (IAA), in the control of harpin-stimulated HR was investigated. IAA was used to inhibit HR that was stimulated by purified fusion harpinXoo protein in tobacco. Semi-quantitative PCR and qRT-PCR were employed to detect the expression of HR related genes. IAA at 100 μM reversed harpin-induced HR which was inhibited by 500 μM 2,3,5-triiodobenzoic acid (TIBA). Semi-quantitative PCR and qRT-PCR showed the combined application of 100 μM IAA and harpin protein from Xanthomonas oryzae enhanced the expression of HR marker gene, hsr203J, but weakened the expression of the disease-defense gene, chia5. TIBA also decreased the expression of hsr203J but increased the expression of chia5. Thus, the auxin can reverse harpinXoo-induced HR.

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Acknowledgments

This work was supported by grants from National Natural Science Foundation of China (31160359, 31360029); Doctoral Fund of Ministry of Education (20104601110004, 20124601110004); National Key Basic Research Plan of China (2011CB111612), National agricultural industrial technology system (ARS-34-GW8); research and start-up capital of Hainan University (kyqd1006). We are particularly grateful to Dr. R. H. Yan, for the helpful comments on the manuscript.

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Authors and Affiliations

  1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource/College of Environment and Plant Protection, Hainan University, Haikou, 570228, China

    Jie Song, Wei-guo Miao & Fu-cong Zheng

  2. Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China

    Xiao-chong Gong, Cong-feng Song & Ming-hua Wang

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  1. Jie Song
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  2. Xiao-chong Gong
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  3. Wei-guo Miao
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  4. Fu-cong Zheng
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  5. Cong-feng Song
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  6. Ming-hua Wang
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Corresponding author

Correspondence to Wei-guo Miao.

Additional information

Jie Song and Xiao-chong Gong contributed to this work equally.

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Song, J., Gong, Xc., Miao, Wg. et al. Indole-3-acetic acid reverses the harpin-induced hypersensitive response and alters the expression of hypersensitive-response-related genes in tobacco. Biotechnol Lett 36, 1043–1048 (2014). https://doi.org/10.1007/s10529-013-1436-7

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  • DOI: https://doi.org/10.1007/s10529-013-1436-7

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