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Human papillomavirus infection and papillary squamous cell carcinoma in the head and neck region

  • Research Article
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Tumor Biology

Abstract

Papillary squamous cell carcinoma (PSCC) is a rare variant of SCC in the head and neck region. The role of human papillomavirus (HPV) infection in PSCC is still unclear. We retrospectively reviewed 11 PSCCs in our institute over a 21-year period and compared the HPV status of PSCCs with 26 squamous cell papillomas (SCPs). Polymerase chain reaction (PCR) amplification to detect HPV DNA and in situ hybridization (ISH) were performed to analyze the relationship between the papillary lesions and HPV infection. Immunohistochemical (IHC) staining for p16 protein expression was used to analyze the PSCC specimens. Nine of 11 (82 %), eight of 11 (73 %), and eight of 11 (73 %) PSCC samples were found to be HPV positive by PCR, ISH, and IHC staining for p16 protein expression, respectively. PSCC had a significantly higher rate of HPV infection than SCP by PCR (p = 0.002) and ISH (p = 0.001) analysis. This study presents different HPV status in two papillary neoplasms and may help to clarify the unique morphological and biological characteristics of head and neck PSCC.

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References

  1. Crissman JD, Kessis T, Shah KV, Fu YS, Stoler MH, Zarbo RJ, et al. Squamous papillary neoplasia of the adult upper aerodigestive tract. Hum Pathol. 1988;19:1387–96.

    Article  PubMed  CAS  Google Scholar 

  2. Ishiyama A, Eversole LR, Ross DA, Raz Y, Kerner MM, Fu YS, et al. Papillary squamous neoplasms of the head and neck. Laryngoscope. 1994;104:1446–52.

    Article  PubMed  CAS  Google Scholar 

  3. Colby C, Klein AM. Papillary squamous cell carcinoma of the larynx. Ear Nose Throat J. 2011;90:E13–5.

    PubMed  Google Scholar 

  4. Ferlito A, Devaney KO, Rinaldo A, Putzi MJ. Papillary squamous cell carcinoma versus verrucous squamous cell carcinoma of the head and neck. Ann Otol Rhinol Laryngol. 1999;108:318–22.

    PubMed  CAS  Google Scholar 

  5. Cobo F, Talavera P, Concha A. Review article: relationship of human papillomavirus with papillary squamous cell carcinoma of the upper aerodigestive tract: a review. Int J Surg Pathol. 2008;16:127–36.

    Article  PubMed  Google Scholar 

  6. Hwang CF, Huang CC, Chien CY, Huang SC, Yang CH, Su CY. Human papillomavirus infection in oral papillary and verrucous lesions is a prognostic indicator of malignant transformation. Cancer Epidemiol. 2012;36:e122–7.

    Article  PubMed  Google Scholar 

  7. Al-Swiahb JN, Huang CC, Fang FM, Chuang HC, Huang HY, Luo SD, et al. Prognostic impact of p16, p53, epidermal growth factor receptor, and human papillomavirus in oropharyngeal cancer in a betel nut-chewing area. Arch Otolaryngol Head Neck Surg. 2010;136:502–8.

    Article  PubMed  Google Scholar 

  8. O'Regan EM, Toner ME, Finn SP, Fan CY, Ring M, Hagmar B, et al. P16(ink4a) genetic and epigenetic profiles differ in relation to age and site in head and neck squamous cell carcinomas. Hum Pathol. 2008;39:452–8.

    Article  PubMed  Google Scholar 

  9. Mirhashemi R, Ganjei-Azar P, Nadji M, Lambrou N, Atamdede F, Averette HE. Papillary squamous cell carcinoma of the uterine cervix: an immunophenotypic appraisal of 12 cases. Gynecol Oncol. 2003;90:657–61.

    Article  PubMed  Google Scholar 

  10. Suarez PA, Adler-Storthz K, Luna MA, El-Naggar AK, Abdul-Karim FW, Batsakis JG. Papillary squamous cell carcinomas of the upper aerodigestive tract: a clinicopathologic and molecular study. Head Neck. 2000;22:360–8.

    Article  PubMed  CAS  Google Scholar 

  11. Thompson LD, Wenig BM, Heffner DK, Gnepp DR. Exophytic and papillary squamous cell carcinomas of the larynx: a clinicopathologic series of 104 cases. Otolaryngol Head Neck Surg. 1999;120:718–24.

    Article  PubMed  CAS  Google Scholar 

  12. Jo VY, Mills SE, Stoler MH, Stelow EB. Papillary squamous cell carcinoma of the head and neck: frequent association with human papillomavirus infection and invasive carcinoma. Am J Surg Pathol. 2009;33:1720–4.

    Article  PubMed  Google Scholar 

  13. Klussmann JP, Weissenborn SJ, Wieland U, Dries V, Kolligs J, Jungehuelsing M, et al. Prevalence, distribution, and viral load of human papillomavirus 16 DNA in tonsillar carcinomas. Cancer. 2001;92:2875–84.

    Article  PubMed  CAS  Google Scholar 

  14. Venuti A, Badaracco G, Rizzo C, Mafera B, Rahimi S, Vigili M. Presence of HPV in head and neck tumours: high prevalence in tonsillar localization. J Exp Clin Cancer Res. 2004;23:561–6.

    PubMed  CAS  Google Scholar 

  15. Paz IB, Cook N, Odom-Maryon T, Xie Y, Wilczynski SP. Human papillomavirus (HPV) in head and neck cancer. An association of HPV 16 with squamous cell carcinoma of Waldeyer's tonsillar ring. Cancer. 1997;79:595–604.

    Article  PubMed  CAS  Google Scholar 

  16. Cobo F, Garcia C, Talavera P, Bravo J, Cabrera C, Concha A. Human papillomavirus associated with papillary squamous cell carcinoma of the oropharynx in a renal transplant recipient. Infection. 2006;34:176–80.

    Article  PubMed  CAS  Google Scholar 

  17. Reed AL, Califano J, Cairns P, Westra WH, Jones RM, Koch W, et al. High frequency of p16 (CDKN2/MTS-1/INK4A) inactivation in head and neck squamous cell carcinoma. Cancer Res. 1996;56:3630–3.

    PubMed  CAS  Google Scholar 

  18. Rawnsley JD, Srivatsan ES, Chakrabarti R, Billings KR, Wang MB. Deletion analysis of the p16/CDKN2 gene in head and neck squamous cell carcinoma using quantitative polymerase chain reaction method. Arch Otolaryngol Head Neck Surg. 1997;123:863–7.

    Article  PubMed  CAS  Google Scholar 

  19. Ai L, Stephenson KK, Ling W, Zuo C, Mukunyadzi P, Suen JY, et al. The p16 (CDKN2A/INK4A) tumor-suppressor gene in head and neck squamous cell carcinoma: a promoter methylation and protein expression study in 100 cases. Mod Pathol. 2003;16:944–50.

    Article  PubMed  Google Scholar 

  20. Sano T, Oyama T, Kashiwabara K, Fukuda T, Nakajima T. Expression status of p16 protein is associated with human papillomavirus oncogenic potential in cervical and genital lesions. Am J Pathol. 1998;153:1741–8.

    Article  PubMed  CAS  Google Scholar 

  21. Klussmann JP, Gultekin E, Weissenborn SJ, Wieland U, Dries V, Dienes HP, et al. Expression of p16 protein identifies a distinct entity of tonsillar carcinomas associated with human papillomavirus. Am J Pathol. 2003;162:747–53.

    Article  PubMed  CAS  Google Scholar 

  22. Li W, Thompson CH, Cossart YE, O'Brien CJ, McNeil EB, Scolyer RA, et al. The expression of key cell cycle markers and presence of human papillomavirus in squamous cell carcinoma of the tonsil. Head Neck. 2004;26:1–9.

    Article  PubMed  Google Scholar 

  23. El-Naggar AK, Lai S, Clayman GL, Zhou JH, Tucker SA, Myers J, et al. Expression of p16, Rb, and cyclin D1 gene products in oral and laryngeal squamous carcinoma: biological and clinical implications. Hum Pathol. 1999;30:1013–8.

    Article  PubMed  CAS  Google Scholar 

  24. Pande P, Mathur M, Shukla NK, Ralhan R. Prb and p16 protein alterations in human oral tumorigenesis. Oral Oncol. 1998;34:396–403.

    Article  PubMed  CAS  Google Scholar 

  25. Higuchi E, Oridate N, Homma A, Suzuki F, Atago Y, Nagahashi T, et al. Prognostic significance of cyclin D1 and p16 in patients with intermediate-risk head and neck squamous cell carcinoma treated with docetaxel and concurrent radiotherapy. Head Neck. 2007;29:940–7.

    Article  PubMed  Google Scholar 

  26. Chien CY, Su CY, Fang FM, Huang HY, Chuang HC, Chen CM, et al. Lower prevalence but favorable survival for human papillomavirus-related squamous cell carcinoma of tonsil in taiwan. Oral Oncol. 2008;44:174–9.

    Article  PubMed  CAS  Google Scholar 

  27. Campbell AC, Gleich LL, Barrett WL, Gluckman JL. Cancerous seeding of the tracheotomy site in patients with upper aerodigestive tract squamous cell carcinoma. Otolaryngol Head Neck Surg. 1999;120:601–3.

    Article  PubMed  CAS  Google Scholar 

  28. Mork J, Lie AK, Glattre E, Hallmans G, Jellum E, Koskela P, et al. Human papillomavirus infection as a risk factor for squamous-cell carcinoma of the head and neck. N Engl J Med. 2001;344:1125–31.

    Article  PubMed  CAS  Google Scholar 

  29. Smith EM, Hoffman HT, Summersgill KS, Kirchner HL, Turek LP, Haugen TH. Human papillomavirus and risk of oral cancer. Laryngoscope. 1998;108:1098–103.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We would like to thank the Chang Gung Medical Foundation Kaohsiung Chang Gung Memorial Hospital Tissue Bank (CLRPG870462) for the technical support. The authors also thank Ching-Mei Chen for her contributions to this project.

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Authors and Affiliations

  1. Department of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, Republic of China

    Chao-Hui Yang & Chung-Feng Hwang

  2. Graduate Institute of Clinical Medical Sciences, Chang Gung University College of Medicine, Kaohsiung, Taiwan, Republic of China

    Chao-Hui Yang, Chao-Cheng Huang & Ming-Tse Ko

  3. Department of Pathology, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, Republic of China

    Chao-Cheng Huang & Yu-Ching Wei

  4. Chang Gung University College of Medicine, No. 123, Ta-Pei Road, Niao-Sung Distinct, Kaohsiung City, Taiwan, Republic of China

    Chao-Hui Yang, Chao-Cheng Huang, Yu-Ching Wei & Chung-Feng Hwang

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  1. Chao-Hui Yang
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  2. Chao-Cheng Huang
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Correspondence to Chao-Cheng Huang or Chung-Feng Hwang.

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Chung-Feng Hwang and Chao-Cheng Huang contributed equally to this manuscript.

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Yang, CH., Huang, CC., Ko, MT. et al. Human papillomavirus infection and papillary squamous cell carcinoma in the head and neck region. Tumor Biol. 34, 301–307 (2013). https://doi.org/10.1007/s13277-012-0551-8

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  • DOI: https://doi.org/10.1007/s13277-012-0551-8

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