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Selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (GTG)5-PCR fingerprinting

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Abstract

The aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (LAB) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. Antimicrobial activity of LAB was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of Bacillus cereus, Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Salmonella Typhimurium. Antifungal activity was tested against the foodborne isolates of Candida parapsilosis, Debaromyces hansenii, Kluyveromyces marxianus, Pichia guilliermondii, Yarowia lipolytica, Aspergillus brasiliensis, Aspergillus versicolor, Cladosporium herbarum, Penicillium chrysogenum and Scopulariopsis brevicaulis.

A total 40 LAB strains representing Lactobacillus (23 strains), Lactococcus (13 strains) and Streptococcus spp. (4 strains) were characterised by repetitive sequence based polymerase chain reaction fingerprinting which generated highly discriminatory profiles, confirmed the identity and revealed high genotypic heterogeneity among the strains. Many of tested LAB demonstrated strong antimicrobial activity specialised against one or few indicator strains. Twelve LAB strains were superior in suppressing growth of the whole complex of pathogenic bacteria and fungi. These results demonstrated that separate taxonomic units offered different possibilities of selection for novel LAB strains could be used as starter cultures enhancing food preservation.

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Acknowledgments

We greatly acknowledge financial support from the Research Council of Lithuania, grant SVE-05/2012 PIENRUGBAKT

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Authors and Affiliations

  1. Kaunas University of Technology, Food institute, Taikos av. 92, LT, 50118, Kaunas, Lithuania

    Joana Šalomskienė, Asta Abraitienė, Dovilė Jonkuvienė & Irena Mačionienė

  2. Vilnius University, Institute of Biotechnology, Vilnius, Lithuania

    Asta Abraitienė

  3. Nature Research Center, Vilnius, Lithuania

    Jūratė Repečkienė

Authors
  1. Joana Šalomskienė
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  2. Asta Abraitienė
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  3. Dovilė Jonkuvienė
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  4. Irena Mačionienė
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  5. Jūratė Repečkienė
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Correspondence to Dovilė Jonkuvienė.

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Šalomskienė, J., Abraitienė, A., Jonkuvienė, D. et al. Selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (GTG)5-PCR fingerprinting. J Food Sci Technol 52, 4124–4134 (2015). https://doi.org/10.1007/s13197-014-1512-6

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  • DOI: https://doi.org/10.1007/s13197-014-1512-6

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