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Optimization of Temperature, Relative Humidity and Storage Time before and after Packaging of Baby Spinach Leaves Using Response Surface Methodology

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Abstract

Second-order polynomial models were used to relate the independent variables, temperature, relative humidity and storage time, to the quality attributes (electrolyte leakage, chlorophylls, weight loss, humidity and headspace gas composition inside packages before and after packaging) of ready-to-eat spinach leaves. Results show that while waiting for processing the best conditions to assure high quality spinach leaves before packaging were 4 °C, 83 % RH and 4 h. The weight loss of spinach leaves was time-dependent: increasing the exposure time under these conditions decreased the amount of wet leaves before packaging. Under the same conditions, the electrolyte leakage was lower (4.63 %) and the chlorophyll content (45.44) was higher. After packaging and while waiting for dispatch, temperature most affected the package headspace atmosphere. Indeed, an increase in storage temperature from 3 to 7 °C led to a significant increase in respiration. The best combination of conditions in the high-care room was 3 °C, 74 % relative humidity for a maximum of 1 day before dispatch. In this case, the moisture content (0.46 %) and electrolyte leakage (4.16 %) were lower and the chlorophyll content (43.37) and oxygen concentration inside the packages of ready-to-eat spinach leaves (19.55 kPa) were higher.

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References

  • Bajji, M., Kinet, J.-M., & Lutts, S. (2002). The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Plant Growth Regulation, 36, 61–70.

    Article  CAS  Google Scholar 

  • Balasubramaniam, S., & Willis, A. (1969). Stomatal movements and rates of gaseous exchange in excised leaves of Vicia faba. New Phytologist, 68, 663–674.

    Article  Google Scholar 

  • Bergquist, S. Å., Gertsson, U. E., & Olsson, M. E. (2006). Influence of growth stage and postharvest storage on ascorbic acid and carotenoid content and visual quality of baby spinach (Spinacia oleracea L.). Journal of the Science of Food and Agriculture, 86, 346–355.

    Article  CAS  Google Scholar 

  • Brecht, J. K. (1995). Physiology of lightly processed fruits and vegetables. Hortscience, 30, 18–22.

    Google Scholar 

  • Burton, W. G. (1982). Post-harvest physiology of food crops. Longman Group Ltd.

  • Cantwell, M., Rovelo, J., Nie, X., & Rubatzky, V. (1998). Specialty salad greens: postharvest physiology and shelf-life. Acta Horticulturae, 371–378.

  • Conte, A., Conversa, G., Scrocco, C., Brescia, I., Laverse, J., Elia, A., & Del Nobile, M. A. (2008). Influence of growing periods on the quality of baby spinach leaves at harvest and during storage as minimally processed produce. Postharvest Biology and Technology, 50, 190–196.

    Article  Google Scholar 

  • Fan, X., & Sokorai, K. J. B. (2005). Assessment of radiation sensitivity of fresh-cut vegetables using electrolyte leakage measurement. Postharvest Biology and Technology, 36, 191–197.

    Article  CAS  Google Scholar 

  • Galindo, F. G., Elias, L., Gekas, V., Herppich, W. B., Smallwood, M., Sommarin, M., Worrall, D., & Sjöholm, I. (2005). On the induction of cold acclimation in carrots (Daucus carota L.) and its influence on storage performance. Food Research International, 38, 29–36.

    Article  Google Scholar 

  • Garrido, Y., Tudela, J. A., & Gil, M. I. (2015). Time of day for harvest and delay before processing affect the quality of minimally processed baby spinach. Postharvest Biology and Technology, 110, 9–17.

    Article  Google Scholar 

  • Garty, J., Weissman, L., Tamir, O., Beer, S., Cohen, Y., Karnieli, A., & Orlovsky, L. (2000). Comparison of five physiological parameters to assess the vitality of the lichen Ramalina lacera exposed to air pollution. Physiologia Plantarum, 109, 410–418.

    Article  CAS  Google Scholar 

  • Guerzoni, M. E., Gianotti, A., Corbo, M. R., & Sinigaglia, M. (1996). Shelf-life modelling for fresh-cut vegetables. Postharvest Biology and Technology, 9, 195–207.

    Article  Google Scholar 

  • Hodges, D. M., Forney, C. F., & Wismer, W. (2000). Processing line effects on storage attributes of fresh-cut spinach leaves. Hortscience, 35, 1308–1311.

    CAS  Google Scholar 

  • Huyskens-Keil, S., & Schreiner, M. (2004). Quality dynamics and quality Assurance of Fresh Fruits and Vegetables in pre- and postharvest. In R. Dris & S. M. Jain (Eds.), (pp. 401–449). Springer Netherlands: Production Practices and Quality Assessment of Food Crops.

    Google Scholar 

  • Kader, A. A. (2002). Biology and technology: an overview. Postharvest Technology Horticultura Crops, 3311, 39–48.

    Google Scholar 

  • Lewicki, P. P. (1998). Effect of pre-drying treatment, drying and rehydration on plant tissue properties: a review. International Journal of Food Properties, 1, 1–22.

    Article  Google Scholar 

  • Limbo, S., & Piergiovanni, L. (2006). Shelf life of minimally processed potatoes: part 1. Effects of high oxygen partial pressures in combination with ascorbic and citric acids on enzymatic browning. Postharvest Biology and Technology, 39, 254–264.

    Article  Google Scholar 

  • Limbo, S., & Piergiovanni, L. (2007). Minimally processed potatoes: part 2. Effects of high oxygen partial pressures in combination with ascorbic and citric acid on loss of some quality traits. Postharvest Biology and Technology, 43, 221–229.

    Article  CAS  Google Scholar 

  • Medina, M. S., Tudela, J. A., Marín, A., Allende, A., & Gil, M. I. (2012). Short postharvest storage under low relative humidity improves quality and shelf life of minimally processed baby spinach (Spinacia oleracea L.). Postharvest Biology and Technology, 67, 1–9.

    Article  CAS  Google Scholar 

  • Ozkan, I. A., Akbudak, B., & Akbudak, N. (2007). Microwave drying characteristics of spinach. Journal of Food Engineering, 78, 577–583.

    Article  Google Scholar 

  • Pandrangi, S., & LaBorde, L. (2004). Retention of folate, carotenoids, and other quality characteristics in commercially packaged fresh spinach. Journal of Food Science, 69, 702–707.

    Article  Google Scholar 

  • Saenmuang, S., Al-Haq, M., Samarakoon, H., Makino, Y., Kawagoe, Y., & Oshita, S. (2012). Evaluation of models for spinach respiratory metabolism under low oxygen atmospheres. Food and Bioprocess Technology, 5, 1950–1962.

    Article  CAS  Google Scholar 

  • Saltveit, M. E. (2002). The rate of ion leakage from chilling-sensitive tissue does not immediately increase upon exposure to chilling temperatures. Postharvest Biology and Technology, 26, 295–304.

    Article  CAS  Google Scholar 

  • Suich, R., & Derringer, G. C. (1980). Is the regression equation adequate?—a further note. Technometrics, 22, 125–126.

    Article  Google Scholar 

  • Tano, K., Oulé, M. K., Doyon, G., Lencki, R. W., & Arul, J. (2007). Comparative evaluation of the effect of storage temperature fluctuation on modified atmosphere packages of selected fruit and vegetables. Postharvest Biology and Technology, 46, 212–221.

    Article  CAS  Google Scholar 

  • Tietel, Z., Lewinsohn, E., Fallik, E., & Porat, R. (2012). Importance of storage temperatures in maintaining flavor and quality of mandarins. Postharvest Biology and Technology, 64, 175–182.

    Article  CAS  Google Scholar 

  • USDA (United States Department of Agriculture) (1987) Soil mechanics level I-module 3: USDA textural classification study guide. National Employee Development Staff, Soil Conservation Service, USDA.

  • Watada, A., Kim, S. D., Kim, K. S., & Harris, T. C. (1987). Quality of green beans, bell peppers and spinach stored in polyethylene bags. Journal of Food Science, 52, 1637–1641.

    Article  Google Scholar 

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Acknowledgments

The work was financially supported by Vitacress Portugal SA through project SETIF 0.004/2015 – New solutions to improve postharvest processability and quality of leafy vegetables. This work was also supported by FCT - Fundação para a Ciência e a Tecnologia through project UID/Multi/50016/2013.

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Correspondence to Ana Oliveira.

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Oliveira, A., Castro, P.M., Amaro, A.L. et al. Optimization of Temperature, Relative Humidity and Storage Time before and after Packaging of Baby Spinach Leaves Using Response Surface Methodology. Food Bioprocess Technol 9, 2070–2079 (2016). https://doi.org/10.1007/s11947-016-1785-z

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  • DOI: https://doi.org/10.1007/s11947-016-1785-z

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