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Effect of diet on the fatty acid and molecular species composition of dog retina phospholipids

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Lipids

Abstract

Dogs were born to mothers fed commercial diets low or enriched in n-3 fatty acids and raised on those diets until they were about 50 d old. Retinas were removed, lipids were extracted, and total phospholipids were anlyzed for fatty acid and molecular species composition. Animals from the low n-3 group had significantly lower retinal levels of 22∶6n-3 and higher levels of n-6 fatty acids, especially 20∶4n-6 and 22∶5n-6. There was no difference in the retinal levels of 18∶2n-6, and only small differences were found in saturated and monounsaturated fatty acids. The most dramatic differences in molecular species occurred in 22∶6n-3-22∶6n-3 (4.7 vs. 0.8%) and 18∶0-22∶6n-3 (27.6 vs. 14.4%); total molecular species containing 22∶6n-3 were significantly lower in the low n-3 group (45.5 vs. 24.0%). Molecular species containing 20∶4n-6 and 22∶5n-6 were greater in the low n-3 animals (13.0 vs. 25.7%), as were molecular species containing only saturated and monounsaturated fatty acids (40.8 vs. 35.4%). These results show that modest differences in the amount of n-3 fatty acids in the diets of dogs can have profound effects on the fatty acid and molecular species composition of their retinas.

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Abbreviations

DGBZ:

diacylglyceroben Z Dates

PUFA:

polyunsaturated fatty acid

ROS:

rod outer segment

TLC:

thin-layer chromatography

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Author information

Author notes

  1. Isabelle Delton-Vandenbroucke

    Present address: INSERM U352, Laboratoire de Biochimie et Pharmacologie, INSA-Lyon, Villeurbanne, France

Authors and Affiliations

  1. Department of Ophthalmology, University of Oklahoma Health Sciences Center, 608 Stanton L. Young Blvd., 73104, Oklahoma City, Oklahoma

    Isabelle Delton-Vandenbroucke, Maureen B. Maude, Huiming Chen & Robert E. Anderson

  2. Department of Cell Biology, University of Oklahoma Health Sciences Center, 608 Stanton L. Young Blvd., 73104, Oklahoma City, Oklahoma

    Robert E. Anderson

  3. Department of Biochemistry & Molecular Biology, University of Oklahoma Health Sciences Center, 608 Stanton L. Young Blvd., 73104, Oklahoma City, Oklahoma

    Robert E. Anderson

  4. Department of Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, 608 Stanton L. Young Blvd., 73104, Oklahoma City, Oklahoma

    Isabelle Delton-Vandenbroucke, Maureen B. Maude, Huiming Chen & Robert E. Anderson

  5. Dean A. McGee Eye Institute, 73104, Oklahoma City, Oklahoma

    Isabelle Delton-Vandenbroucke, Maureen B. Maude, Huiming Chen & Robert E. Anderson

  6. James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, 14853, Ithaca, New York

    Gustavo D. Aguirre & Gregory M. Acland

Authors
  1. Isabelle Delton-Vandenbroucke
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  2. Maureen B. Maude
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  3. Huiming Chen
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  4. Gustavo D. Aguirre
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  5. Gregory M. Acland
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Delton-Vandenbroucke, I., Maude, M.B., Chen, H. et al. Effect of diet on the fatty acid and molecular species composition of dog retina phospholipids. Lipids 33, 1187–1193 (1998). https://doi.org/10.1007/s11745-998-0322-7

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  • DOI: https://doi.org/10.1007/s11745-998-0322-7

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