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Micropropagation of Mandevilla moricandiana (A.DC.) Woodson

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Abstract

A protocol was developed for micropropagation of Mandevilla moricandiana (A.DC.) Woodson, a native plant from Brazil. Shoots, obtained from in vitro plantlets were used as source of nodal segments for shoot production from axillary buds. The nodal segments were grown on Murashige and Skoog medium supplemented with different concentrations of 6-benzyladenine and/or indole-3-acetic acid to induce axillary bud elongation. After a 2-mo culture period, the medium supplemented with 1.0 mg L−1 6-benzyladenine gave the largest number of nodal segments per explant. The nodal segments obtained from plants developed under these conditions were grown on medium supplemented with different concentrations indole-3-acetic acid, α-naphthaleneacetic acid, and indole-3-butyric acid. The use of the medium supplemented with indole-3-acetic acid and indole-3-buryric induced shoot elongation and shoot development, formation of basal callus, and/or indirect organogenesis of roots. Following transfer of shoots to soil, the plants with only basal callus showed 10% survival and developed roots from callus, while in vitro-rooted plants had a maximum 40% survival rate ex vitro. Regardless of the auxin added to the rooting medium, the acclimatization period allowed the plants rooted in vitro to develop their shoots fully. The protocol developed here is suitable for the production of shoots and rooted plantlets of M. moricandiana.

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Acknowledgments

The authors thank the Conselho de Administração de Pessoal de Ensino Superior for a doctoral scholarship for the first author, Programa de Pós-graduação em Biotecnologia Vegetal, Universidade Federal do Rio de Janeiro and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro for financial support, Dr. Tatiana Ungaretti Paleo Konno of the UFRJ-Macaé for providing seeds of M. moricandiana, taxonomists Dr. Jorge Fontella Pereira of the Museu Nacional (UFRJ), Marcelo Fraga Castilhiori and Inaldo do Espírito Santo of the Herbarium Bradeanum for species identification, Universidade Federal do Estado do Rio de Janeiro (UNIRIO) for providing transport to the collection areas, IBAMA-Brazilian Institute for Environment and Natural Renewable Resources-for authorization to collect (Scientific Research Activities no. 18498-1), and the anonymous reviewers for their valuable comments and suggestions to improve the manuscript.

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Authors and Affiliations

  1. Post-graduation in Plant Biotechnology, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 373 Cidade Universitária, 21941-902, Rio de Janeiro, Rio de Janeiro, Brazil

    Sandra Zorat Cordeiro

  2. Department of Natural Products and Food–College of Pharmacy, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 373 Cidade Universitária, 21941-902, Rio de Janeiro, Rio de Janeiro, Brazil

    Naomi Kato Simas

  3. Laboratory of Plant Development Physiology–Institute of Biology, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 373 Cidade Universitária, 21941-902, Rio de Janeiro, Rio de Janeiro, Brazil

    Anaize Borges Henriques

  4. National Institute of Industrial Property, Rua Mayrink Veiga, 9, 20090-910, Rio de Janeiro, Rio de Janeiro, Brazil

    Celso Luiz Salgueiro Lage

  5. Laboratory of Plant Tissue Culture–Department of Botany, Federal University of the State of Rio de Janeiro, Av. Pasteur 458, 22290-040, Rio de Janeiro, Rio de Janeiro, Brazil

    Alice Sato

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  1. Sandra Zorat Cordeiro
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  2. Naomi Kato Simas
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  3. Anaize Borges Henriques
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  4. Celso Luiz Salgueiro Lage
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  5. Alice Sato
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Correspondence to Sandra Zorat Cordeiro.

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Editor: Rakhi Chaturvedi

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Cordeiro, S.Z., Simas, N.K., Henriques, A.B. et al. Micropropagation of Mandevilla moricandiana (A.DC.) Woodson. In Vitro Cell.Dev.Biol.-Plant 48, 620–626 (2012). https://doi.org/10.1007/s11627-012-9477-5

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  • DOI: https://doi.org/10.1007/s11627-012-9477-5

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