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Expression of Stat3 in Feline Mammary Gland Tumours and its Relation to Histological Grade

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Abstract

The expression of Stat3 (signal transducer and activator of transcription 3) in normal and neoplastic feline mammary gland tissue was assessed by immunohistochemistry in 72 cats. The samples included 3 normal nonlactating mammary tissues, 17 hyperplastic lesions (11 lobular and 6 fibroepithelial) and 52 neoplasms (5 benign and 47 malignant). For immunohistochemistry, tissue sections were incubated with anti-Stat3 monoclonal antibody and visualized with EnVision–DAB polymer. Stat3 positivity was assessed in a semiquantitative manner. Normal mammary tissue showed occasional cytoplasmic and nuclear positivity, although the number of positive cells was lower than in the hyperplastic specimens. In neoplastic tissue, high numbers of neoplastic cells with a moderate to intense cytoplasmic and nuclear positivity were observed. Selected variations in the staining pattern correlated with malignancy. Moreover, a positive association was seen between the histological grade and the Stat3 score (p < 0.0001; correlation coefficient 0.750). A linear regression model showed a positive association between mitotic count and Stat3 expression (p < 0.001). These results further support published data found in humans regarding to the relation between the expression of Stat3 and malignancy.

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Abbreviations

ASC:

adenosquamous carcinoma

BSA:

bovine serum albumin

CA:

complex adenoma

CC:

carcinoma cribriforme

CSF-1:

colony-stimulating factor-1

DAB:

3,3′-diaminobenzide tetrahydrochloride

DC:

duct papilloma

DSH:

domestic Short Hair

EGFr:

epidermal growth factor receptor

IFN:

interferon

F:

female

FEH:

fibroepithelial hyperplasia

FS:

female spayed

LEH:

lobular epithelial hyperplasia

NMGT:

normal mammary gland tissue

NR:

not reported

PC:

Persian cat

PBS:

phosphate-buffered Saline

PDGFR:

platelet-derived growth factor receptor

SA:

simple adenoma

SCS:

simple carcinoma solid

SCT:

simple carcinoma tubular

SCTP:

simple carcinoma tubular papillary

Stat3:

signal transducer and activator of transcription 3

TBS:

Tris-buffered saline

TNF:

tumour necrosis factor

WHO:

World Health Organization

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Authors and Affiliations

  1. Department of Public Health, Comparative Pathology and Veterinary Hygiene School of Veterinary Medicine, AGRIPOLIS, Legnaro, University of Padua, Padua

    C. Petterino, E. Arcicasa & M. Drigo

  2. Istituto Zooprofilattico Sperimentale, Liguria, Piemonte, Valle D’Aosta, National Reference Centre of Veterinary and Comparative Oncology, Genoa, Italy

    A. Ratto, G. Podestà & C. Pellegrino

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  1. C. Petterino
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  2. A. Ratto
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  3. E. Arcicasa
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  4. G. Podestà
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  5. M. Drigo
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  6. C. Pellegrino
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Correspondence to C. Petterino.

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Petterino, C., Ratto, A., Arcicasa, E. et al. Expression of Stat3 in Feline Mammary Gland Tumours and its Relation to Histological Grade. Vet Res Commun 30, 599–611 (2006). https://doi.org/10.1007/s11259-006-3335-z

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  • DOI: https://doi.org/10.1007/s11259-006-3335-z

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