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Determination of antioxidant activity and phenolic content of extracts from in vivo plants and in vitro materials of Passiflora alata Curtis

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Abstract

Passiflora alata Curtis, commonly known as sweet passion fruit, is one of the commercially cultivated species of the genus Passiflora, whose fruits can be consumed in natura due to their sweet taste. It is also used worldwide as an ornamental and in folk medicine. The goal of this work was the evaluation of the antioxidant potential of extracts from in vivo plants, and in vitro-derived materials of P. alata. Leaves from in vivo plants were used for the optimization of parameters that affect the efficiency of extraction of antioxidant compounds (proportions of ethanol:water, maceration period, solvent:plant tissue ratio, and number of extraction stages), by employing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The antioxidant activity and the extract yields were significantly influenced by the proportion of ethanol:water and maceration period. The optimized protocol was applied to obtain the extracts of in vitro-derived materials. Total phenolic content was determined using the Folin–Ciocalteu method. Higher antioxidant activities and phenolic contents were observed in extracts from leaves of in vivo-seed derived and from acclimatized plants when compared to in vitro plants, calluses and suspension cultures. Differences in the reaction kinetics of DPPH scavenging activity were also observed.

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References

  • Antognoni F, Zheng S, Pagnucco C, Baraldi R, Poli F, Biondi S (2007) Induction of flavonoid prodiction by UV-B radiation in Passiflora quadrangularis callus cultures. Fitoterapia 78:345–352

    Article  CAS  PubMed  Google Scholar 

  • Bernabe-Antonio A, Estrada-Zunika ME, Buendia-Gonzales L, Reyes-Chilpa R, Cruz-Soza F (2010) Production of anti-HIV-1 calanolides in a callus culture of Calophyllum brasiliense (Cambes). Plant Cell Tissue Organ Cult 103:33–40

    Article  CAS  Google Scholar 

  • Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Food Sci Technol 28:25–30

    CAS  Google Scholar 

  • Chabariberi RAO, Pozzi ACS, Zeraik ML, Yariwake JH (2009) Spectrometric determination of flavonoids from Maytenus (Celastraceae) and Passiflora (Passifloraceae) leaves and comparison with an HPLC-UV method. Braz J Pharmacogn 9:860–864

    Google Scholar 

  • Dhawan K, Dhawan S, Sharma A (2004) Passiflora: a review update. J Ethnopharmacol 94:1–23

    Article  CAS  PubMed  Google Scholar 

  • Doyama JT, Rodrigues HG, Novelli EL, Cereda E, Vilegas W (2005) Chemical investigation and effects of the tea of Passiflora alata on biochemical parameters in rats. J Ethnopharmacol 96:371–374

    Article  CAS  PubMed  Google Scholar 

  • Ferreres F, Souza C, Valentão P, Andrade PB, Seabra RM, Gil-Izquierdo A (2007) New C-deoxyhexosyl flavones and antioxidant properties of Passiflora edulis leaf extract. J Agric Food Chem 55:10187–10193

    Article  CAS  PubMed  Google Scholar 

  • Fraccaroli M, Nicoletti S, Maltese F, Choi YH, Guzzo F, Levi M, Verpoorte R (2008) Pre-analytical method for metabolic profiling of plant cell cultures of Passiflora garckei. Biotechnol Lett 30:2031–2036

    Article  CAS  PubMed  Google Scholar 

  • George EF (2008) Plant tissue culture procedure—Background. In: George EF, Hall MA, De Klerk GJ (eds) Plant propagation by tissue culture, 3rd edn, vol. 1. Springer, The Netherlands

  • Grzegorczyk I, Matkowski A, Wysokinska H (2007) Antioxidant activity of extracts from in vitro cultures of Salvia officinalis L. Food Chem 104:536–541

    Article  CAS  Google Scholar 

  • Holland KW, Balota M, Eigel WN et al (2011) ORAChromatography and total phenolics content of peanut root extracts. J Food Sci 76:C380–C384

    Article  CAS  PubMed  Google Scholar 

  • Hussein EA, Taj-Eldeen AM, Al-Zubairi AS, Elhakimi AS, Al-Dubaie AR (2010) Phytochemical screening, total phenolics and antioxidant and antibacterial activities of callus from Brassica nigra L. hypocotyl explants. Int J Pharmacol 6:464–471

    Article  Google Scholar 

  • Jacob A, Malpathak N (2004) Green hairy root cultures of Solanum khasianum Clarke—a new route to in vitro solasodine production. Current Sci 87:1442–1447

    CAS  Google Scholar 

  • Kim S, Yang M, Lee O, Kang S (2011) The antioxidant activity and the bioactive compound content of Stevia rebaudiana water extracts. Food Sci Technol 44:1328–1332

    CAS  Google Scholar 

  • Klein N, Gazola AC, de Lima TC, Schenkel E, Nieber K, Butterweck V (2013) Assessment of sedative effects of Passiflora edulis f. flavicarpa and Passiflora alata extracts in mice, measured by telemetry. Phytother Res 26. doi:10.1002/ptr.5043

  • Kris-Etherton PM, Hecker KD, Bonanome A et al (2002) Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. Am J Med 113:71–88

    Article  Google Scholar 

  • Kumar V, Murthy KN, Bhamid S, Sudha CG, Ravishankar GA (2005) Genetically modified hairy roots of Withania somnifera Dunal: a potent source of rejuvenating principles. Rejuvenation Res 8:37–45

    Article  CAS  PubMed  Google Scholar 

  • Kuskoski EM, Asuero AG, Morales MT, Fett R (2006) Wild fruits and pulps of frozen fruits: antioxidant activity, polyphenols and anthocyanins. Cienc Rural 36:1283–1287

    Article  Google Scholar 

  • Liu CZ, Murch SJ, El-Demerdash M, Saxena PK (2004) Artemisia judaica L.: micropropagation and antioxidant activity. J Biotechnol 110:63–71

    Article  CAS  PubMed  Google Scholar 

  • López-Laredo AR, Ramírez-Flores FD, Sepúlveda-Jiménez G, Trejo-Tapia G (2009) Comparison of metabolite levels in callus of Tecoma stans (L.) Juss. ex Kunth. cultured in photoperiod and darkness. In Vitro Cell Dev Biol Plant 45:550–558

    Article  Google Scholar 

  • Lutomski J, Malek B (1975) Pharmacological investigations on the raw material of the genus Passiflora. IV. The comparison of contents of alkaloids in some harman raw materials. Planta Med 27:381–384

    Article  CAS  PubMed  Google Scholar 

  • Machado MW, Neto CS, Salgado J et al (2010) Search for alkaloids on callus culture of Passiflora alata. Braz Arch Biol Technol 53:901–910

    Article  CAS  Google Scholar 

  • Manach C, Scalbert A, Morand C, Rémésy C, Jiménez L (2004) Polyphenols: food sources and bioavaibility. Am J Clin Nutr 79:727–747

    CAS  PubMed  Google Scholar 

  • Monteiro ACB, Higashi EN, Gonçalves AN, Rodriguez APM (2000) A novel approach for the definition of the inorganic medium components for micropropagation of yellow passionfruit (Passiflora edulis sims. F. Flavicarpa Deg.). In Vitro Cell Dev Biol Plant 36:527–531

    Article  Google Scholar 

  • Moraes MLL, Vilegas JHY, Lanças FM (1997) Supercritical fluid extraction of glycosylated flavonoids from Passiflora leaves. Phytochem Anal 8:257–260

    Article  CAS  Google Scholar 

  • Muller SD, Vasconcelos SB, Coelho M, Biavatti MW (2005) LC and UV determination of flavonoids from Passiflora alata medicinal extracts and leaves. J Pharm Biomed Anal 37:399–403

    Article  PubMed  Google Scholar 

  • Nangedran B, Sundran K, Samman S (2006) Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence, and potential uses. Food Chem 99:191–203

    Article  Google Scholar 

  • Nepote V, Grosso NR, Guzmán CA (2005) Optimization of extraction of phenolic antioxidants from peanut skins. J Sci Food Agric 85:33–38

    Article  CAS  Google Scholar 

  • Oga S, de Freitas PC, Gomes da Silva AC, Hanada S (1984) Pharmacological trial of crude extract of Passiflora alata. Planta Med 50:303–306

    Article  CAS  PubMed  Google Scholar 

  • Pacheco G, Garcia R, Lugato D, Vianna M, Mansur E (2012) Plant regeneration, callus induction and establishment of cell suspension cultures of Passiflora alata Curtis. Sci Hortic (Amsterdam) 144:42–47

    Article  CAS  Google Scholar 

  • Pereira CA, Yariwake JH, Lancas FM, Wauters JN, Tits M, Angenot L (2004) A HPTLC densitometric determination of flavonoids from Passiflora alata, P. edulis, P. incamata and P. caerulea and comparison with HPLC method. Phytochem Anal 15:241–248

    Article  CAS  PubMed  Google Scholar 

  • Petry RD, Reginatto F, de Paris F et al (2001) Comparative pharmacological study of hidroethanol extracts of Passiflora alata and Passiflora edulis leaves. Phytother Res 15:162–164

    Article  CAS  PubMed  Google Scholar 

  • Rakotoarison DA, Gressier B, Trotin F et al (1997) Antioxidant activities of polyphenolic extract from flowers, in vitro callus and cell suspension cultures of Crataegus monogyna. Pharm 52:60–64

    CAS  Google Scholar 

  • Reginato FH, De-Paris F, Petry RD, Quevedo J, Ortega GG, Gosmann G, Schenkel EP (2006) Evaluation of anxiolytic activity of spray dried powders of two South Brazilian Passiflora species. Phytother Res 20:348–351

    Article  Google Scholar 

  • Rudnicki M, Oliveira MR, Pereira TV, Reginatto FH, Dal-Pizzol F, Moreira JCF (2007a) Antioxidant and antiglycation properties of Passiflora alata and Passiflora edulis extracts. Food Chem 100:719–724

    Article  CAS  Google Scholar 

  • Rudnicki M, Silveira MM, Pereira TV et al (2007b) Protective effects of Passiflora alata extract pretreatment on carbon tetrachloride induced oxidative damage in rats. Food Chem Toxicol 45:656–661

    Article  CAS  PubMed  Google Scholar 

  • Sánchez-Moreno C, Larrauri JA, Saura-Calixto F (1998) A procedure to measure the antiradical efficiency of polyphenols. J Sci Food Agric 76:270–276

    Article  Google Scholar 

  • Thiem B, Krawczyk A (2003) Ellagic acid in in vitro cultures of Rubus chamaemorus L. Herb Pol 49:202–209

    CAS  Google Scholar 

  • Trajtemberg SP, Apostolo NM, Fernandez G (2006) Calluses of Cynara cardunculus var. cardunculus cardoon (Asteraceae): determination of cynarine and chlorogenic acid by automated high-performance capillary electrophoresis. In Vitro Cell Dev Biol Plant 42:534–537

    Article  CAS  Google Scholar 

  • Ulmer T, MacDougal JM (2004) Passiflora: Passiflowers of the world. Timber Press, Portland

    Google Scholar 

  • Vargas AJ, Geremias DS, Provensi G et al (2007) Passiflora alata and Passiflora edulis spray-dried aqueous extracts inhibit inflammation in mouse model of pleurisy. Fitoterapia 78:112–119

    Article  CAS  PubMed  Google Scholar 

  • Vogelsang K, Schneider B, Petersen M (2006) Production of rosmarinic acid and a newrosmarinic acid 3-O-beta-D-glucoside in suspension cultures of the hornwort Anthoceros agrestis Paton. Planta 223:369–373

    Article  CAS  PubMed  Google Scholar 

  • Wilmsen PK, Spada DS, Salvador M (2005) Antioxidant activity of the flavonoid Hesperidin in chemical and biological systems. J Agric Food Chem 53:4754–4761

    Article  Google Scholar 

  • Yan Q, Shi M, Ng J, Wu JY (2006) Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Salvia miltiorrhiza hairy roots. Plant Sci 170:853–858

    Article  CAS  Google Scholar 

  • Zeraik ML, Yariwake JH (2010) Quantification of isoorientin and total flavonoids in Passiflora edulis fruit pulp by HPLC-UV/DAD. Microchem J 96:86–91

    Article  CAS  Google Scholar 

  • Zhan L, Hu J, Pang L, Li Y, Shao J (2014) Light exposure reduced browning enzyme activity and accumulated total phenols in cauliflower heads during cool storage. Postharvest Biol Technol 88:17–20

    Article  CAS  Google Scholar 

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Acknowledgments

The authors acknowledge the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) for financial suport and a post-doctoral fellowship. Danielle Lugato and Mariela J. Simão are recipients of scholarships from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). E. Mansur is a recipient of a research fellowship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Instituto de Biologia Roberto Alcantara Gomes, Núcleo de Biotecnologia Vegetal, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier no 524 PHLC sala 505, Maracanã, Rio de Janeiro, 20550-013, Brazil

    Danielle Lugato, Mariela J. Simão, Renata Garcia, Elisabeth Mansur & Georgia Pacheco

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  1. Danielle Lugato
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  2. Mariela J. Simão
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  3. Renata Garcia
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  4. Elisabeth Mansur
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  5. Georgia Pacheco
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Correspondence to Georgia Pacheco.

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Lugato, D., Simão, M.J., Garcia, R. et al. Determination of antioxidant activity and phenolic content of extracts from in vivo plants and in vitro materials of Passiflora alata Curtis. Plant Cell Tiss Organ Cult 118, 339–346 (2014). https://doi.org/10.1007/s11240-014-0486-4

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  • DOI: https://doi.org/10.1007/s11240-014-0486-4

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