Abstract
Industrially manufactured titanium dioxide nanoparticles have been successfully radiolabelled with 48V by irradiation with a cyclotron-generated proton beam. Centrifugation tests showed that the 48V radiolabels were stably bound within the nanoparticle structure in an aqueous medium, while X-ray diffraction indicated that no major structural modifications to the nanoparticles resulted from the proton irradiation. In vitro tests of the uptake of cold and radiolabelled nanoparticles using the human cell line Calu-3 showed no significant difference in the uptake between both batches of nanoparticles. The uptake was quantified by Inductively Coupled Plasma Mass Spectrometry and high resolution γ-ray spectrometry for cold and radiolabelled nanoparticles, respectively. These preliminary results indicate that alterations to the nanoparticles’ properties introduced by proton bombardment can be controlled to a sufficient extent that their further use as radiotracers for biological investigations can be envisaged and elaborated.
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References
Aitken RJ, Chaudhry MQ, Boxall ABA, Hull M (2006) Manufacture and use of nanomaterials: current status in the UK and globaltrends. Occup Med 56:300–306
Anselmann R (2001) Nanoparticles and nanolayers in commercial applications. J Nanopart Res 3:329–336
Balbus JM, Florini K, Denison RA, Walsh SA (2007) Protecting workers and the environment: an environmental NGO’s perspective on nanotechnology. J Nanopart Res 9:11–22
Berger JT, Voynow JA, Peters KW, Rose MC (1999) Respiratory carcinoma cell lines. MUC genes and glycoconjugates. Am J Respir Cell Mol Biol 20:500–510
Di Giampaolo L, Di Gioacchino M, Ponti J, Sabbioni E, Castellani ML, Reale M, Toto E, Verna N, Conti P, Paganelli R, Boscolo P (2004) “In vitro” comparative immune effects of different titanium compounds. Int J Immunopathol Pharmacol 17(Suppl 2):115–122
Di Gioacchino M, Verna N, Di Giampaolo L, Di Claudio F, Turi MC, Perrone A, Petrarca C, Mariani-Costantini R, Sabbioni E, Boscolo P (2007) Immunotoxicity and sensitizing capacity of metal compounds depend on speciation. Int J Immunopathol Pharmacol 20(Suppl 2):15–22
Florence A, Hussain N (2001) Transcytosis of nanoparticle and dendrimer delivery systems: evolving vistas. Adv Drug Deliv Rev 50(Suppl):S69–S89
Forbes B (2000) Human airway epithelial cell lines for in vitro drug transport and metabolism studies. Pharm Sci Technol Today 3:18–27
Garabant DH, Fine LJ, Oliver C, Bernstein L, Peters JM (1987) Abnormalities of pulmonary function and pleural disease among titanium metal production workers. Scand J Work Environ Health 13:47–51
Grainger CI, Greenwell LL, Lockley DJ, Martin GP, Forbes B (2006) Culture of Calu-3 cells at the air interface provides a representative model of the airway epithelial barrier. Pharm Res 23:1482–1490
Handy RD, Henry TB, Scrown TM, Johnston BD, Tyler CR (2008a) Manufactured nanoparticles: their uptake and effects on fish—a mechanistic analysis. Ecotoxicology 15:396–409
Handy RD, von der Kammer F, Lead JR, Hassellöv M, Owen R, Crane M (2008b) The ecotoxicology and chemistry of manufactured nanoparticles. Ecotoxicology 17:287–314
IAEA (2008) Experimental Nuclear Reaction Data (EXFOR). http://www-nds.iaea.org/exfor/exfor.htm (continuously updated)
Kam N, O’Connell M, Wisdom J, Dai H (2005) Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer-cell destruction. Proc Natl Acad Sci 102:11600–11605
Keller KH (2007) Nanotechnology and society. J Nanopart Res 9:5–10
Kreyling WG, Semmler M, Möller W (2004) Dosimetry and toxicology of ultrafine particles. J Aerosol Med 17:140–152
Liao C-M, Chiang Y-H, Chio C-P (2009) Assessing the airborne titanium dioxide nanoparticle-related exposure hazard at workplace. J Hazard Mater 162:57–65
Maynard AD, Aitken RJ, Butz T, Colvin V, Donaldsen K, Oberdörster G, Philbert MA, Ryan J, Seaton A, Stone V, Tinkle SS, Tran L, Walker NJ, Warheit DB (2006) Safe handling of nanotechnology. Nature 444:267–269
Oberdörster G, Oberdörster E, Oberdörster J (2005) Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ Health Perspect 113:823–839
OECD (2006) Report of the OECD workshop on the safety of manufactured nanomaterials: co-operation, co-ordination and communication. Organization for Economic Co-operation and Development, Paris
OECD (2008) Current developments/activities on the safety of manufactures nanomaterials. OECD Environment, Health and Safety Publications Series on the Safety of Manufactured Nanomaterials, Paris
Owen R, Depledge M (2005) Nanotechnology in the environment: risks and rewards. Mar Pollut Bull 50:609–612
Pantarotto D, Briand J, Prato M, Bianco A (2004) Translocation of bioactive peptides across cell membranes by carbon nanotubes. Chem Commun (Camb) 1:16–17
Poland CA, Duffin R, Kinloch I, Maynard A, Wallace WAH, Seaton A, Stone V, Brown S, MacNee W, Donaldson K (2008) Carbon nanotubes introduced into the abdominal cavity of mice show asbestos-like pathogenicity in a pilot study. Nat Nanotechnol 3:423–428
Reijnders L (2009) The release of TiO2 and SiO2 nanoparticles from nanocomposites. Polym Degrad Stab 94:873–876
Roco MC (2008) The journal of nanoparticle research at 10 years. J Nanopart Res 10(Suppl 1):1–2
Semmler-Behnke M, Kreyling WG, Lipka J, Fertsch S, Wenk A, Takenaka S, Schmid G, Brandau W (2008) Biodistribution of 1.4- and 18-nm gold particles in rats. Small 4:2108–2111
Steimer A, Haltner E, Lehr CM (2005) Cell culture models of the respiratory tract relevant to pulmonary drug delivery. J Aerosol Med 18:137–182
Vevers WF, Jha AN (2008) Genotoxic and cytotoxic potential of titanium dioxice TiO2 nanoparticles on fish cells in vitro. Ecotoxicology 17:410–420
Wan H, Winton HL, Soeller C, Stewart GA, Thompson PJ, Gruenert DC, Cannell MB, Garrod DR, Robinson C (2000) Tight junction properties of the immortalized human bronchial epithelial cell lines Calu-3 and 16HBE14o-. Eur Respir J 15:1058–1068
Wiesner MR, Lowry GV, Alvarez P, Dionysiou D, Biswas P (2006) Assessing the risks of manufactured nanomaterials. Environ Sci Technol 40:4336–4345
Zhao X, Striolo A, Cummings P (2005) C60 binds to and deforms nucleotides. Biophys J 89:3856–3862
Ziegler JF, Ziegler MD, Biersack JP (2008) SRIM—the stopping and range of ions in matter. Free download possible from http://www.srim.org/
Acknowledgements
The authors would like thank W. Horstmann and F. Arroja for their continuous support in the fabrication and improvement of the design of the irradiation capsules and G. Cotogno for his IT support. This work has been partially funded by the European Commission’s 7th Framework Programme, ‘NeuroNano’ project (contract NMP4-SL-2008-214547).
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Abbas, K., Cydzik, I., Del Torchio, R. et al. Radiolabelling of TiO2 nanoparticles for radiotracer studies. J Nanopart Res 12, 2435–2443 (2010). https://doi.org/10.1007/s11051-009-9806-8
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DOI: https://doi.org/10.1007/s11051-009-9806-8