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Microarray-based screening of the microRNAs associated with caryopsis development in Oryza sativa

  • Original Papers
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Biologia Plantarum

Abstract

Plant microRNAs modulate diverse developmental processes by regulating expression of their target genes. To explore potential miRNA-guided gene regulation in developing rice (Oryza sativa L.) caryopses, a miRNA microarray was used to identify miRNAs present at the different developmental stages. We found that 27 miRNAs, of which 16 were conserved miRNAs, were present in developing caryopses. High expression levels were detected for miR159, miR167, and miR530 at the morphogenesis stage and for miR169, miR435, and miR528 at the stage of accumulation of metabolites. Next, 26 target genes were predicted for seven of the detected miRNAs and the expression profiles of these miRNAs and their corresponding target genes were examined in developing caryopses. Our results suggest that the miRNAs and their target genes examined at the two distinct stages could contribute to the developmental progress of rice caryopses in concert with phytohormone signalling.

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Abbreviations

ABA:

abscisic acid

ARF:

auxin response factor

ASO:

L-ascorbate oxidase

DAF:

days after fertilisation

DAH:

days after heading

GAP:

GTPase activating protein

GPCR:

G protein-coupled receptor

LRR:

leucine-rich repeat

PCD:

programmed cell death

snRNA:

small nuclear RNA

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Authors and Affiliations

  1. Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, 19, XinJieKouWai Avenue, Beijing, 100875, P.R. China

    H. Liu, D. Shen, S. Jia, W. Li, J. Li, J. Liu, S. Han & Y. Wang

Authors
  1. H. Liu
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  2. D. Shen
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  3. S. Jia
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  4. W. Li
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  5. J. Li
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  6. J. Liu
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  7. S. Han
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  8. Y. Wang
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Corresponding authors

Correspondence to S. Han or Y. Wang.

Additional information

Acknowledgments: This work was supported by the National Natural Science Foundation of China (No. 30570148).

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Liu, H., Shen, D., Jia, S. et al. Microarray-based screening of the microRNAs associated with caryopsis development in Oryza sativa . Biol Plant 57, 255–261 (2013). https://doi.org/10.1007/s10535-012-0270-4

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  • DOI: https://doi.org/10.1007/s10535-012-0270-4

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