Abstract
The transcript levels and activities of the superoxide dismutase isoenzyme (Cu/ZnSOD) were assessed in winter (Tarm-92) and spring (Zafer-160) barley cultivars during cold acclimation, freezing stress and after rewarming. Leaf Cu/ZnSOD activity and Cu/ZnSOD expression level were not significantly changed during cold acclimation. The Cu/ZnSOD expression increased evidently at mild freezing stress (−3 °C; F1), while Cu/ZnSOD1 activity did not show any response and Cu/ZnSOD2 activity decreased continuously during F1 and F2 (−7 °C) in Tarm-92. On the other hand, root Cu/ZnSOD2 activity was in accordance with Cu/ZnSOD expression in Zafer-160 after F2 treatment. Rewarming periods did not cause any significant changes in the Cu/ZnSOD activity and expression of Cu/ZnSOD in both cultivars when compared to freezing stresses. These results showed that freezing stress can regulate differently Cu/ZnSOD transcription and enzyme activity.
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Abbreviations
- APX:
-
ascorbate peroxidase
- CA:
-
cold-acclimated
- CAT:
-
catalase
- GR:
-
glutathione reductase
- NBT:
-
nitroblue tetrazolium
- ROS:
-
reactive oxygen species
- RT-PCR:
-
reverse transcription polymerase chain reaction
- SOD:
-
superoxide dismutase
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Acknowledgements: We would like to thank Turkish Ministry of Agriculture and Rural Affairs for barley cultivars Tarm-92 and Zafer-160. This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK TOVAG-105O152).
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Kayihan, C., Eyidogan, F., Afsar, N. et al. Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars. Biol Plant 56, 693–698 (2012). https://doi.org/10.1007/s10535-012-0143-x
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DOI: https://doi.org/10.1007/s10535-012-0143-x