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Origins and distribution of invasive Rubus fruticosus L. agg. (Rosaceae) clones in the Western United States

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Abstract

In facultatively asexual invasive species, an understanding of the origins and diversity of clones can reveal introduction and invasion pathways and inform management efforts. In this study, we use microsatellite and chloroplast DNA markers to infer clonal diversity of the Rubus fruticosus agg. invasion in the Western United States, determine the relationship of these clones to clones found in other exotic ranges, and determine the geographic and genetic origins of the invasive clones. We found two invasive clones in the Western United States, where the invasion had previously been thought to consist of a single asexual lineage. The most common clone was genetically identical to the microspecies R. armeniacus from the native range of Germany, while the second clone was identical to the microspecies R. anglocandicans in the invaded range of Australia and closely related to samples from the native ranges of England and Serbia. A third distinct clone was identified in a collection from the exotic range of Chile. Our results demonstrate that cryptic genetic diversity may be present in asexual invasions that are thought to be homogeneous. However, the asexual relationships between R. fruticosus agg. clones in the native and multiple exotic ranges indicate that preadaptation has played an important role in invasion success in this species aggregate.

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Acknowledgments

Bernie May, Paul Gepts, Kristina Schierenbeck, Associate Editor Richard Lankau and two anonymous reviewers provided insightful comments on earlier versions of this manuscript. Susan Lambrecht, Ramona Robison, Kristina Schierenbeck, Jeff Firestone, Anna Sherwood, Annabelle Kleist, Noor-ul-ain Noor, Cosumnes River Preserve, Caswell Memorial State Park, and Pacific Star Gardens gave assistance and permissions for field collections. Stella Hartono and Isaac Nelson-King assisted with genotyping. The United States Department of Agriculture—National Clonal Germplasm Repository at Corvallis, Oregon kindly provided leaf and seed material for the worldwide Rubus fruticosus agg. dataset. The first author (LVC) was supported on a University of California-Davis Plant Sciences Departmental Graduate Research Assistantship. Research was funded by a Jastro-Shields Research Award and a California Weed Science Society scholarship.

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  1. Lindsay V. Clark

    Present address: Energy Biosciences Institute, Institute for Genomic Biology, University of Illinois, 1206 West Gregory Drive, Urbana, IL, 61801, USA

Authors and Affiliations

  1. Department of Plant Sciences, Mail Stop 4, University of California, Davis, CA, 95616, USA

    Lindsay V. Clark & Marie Jasieniuk

  2. Perennial Horticulture Centre, Tasmanian Institute of Agriculture, University of Tasmania, 13 St Johns Avenue, New Town, TAS, 7008, Australia

    Katherine J. Evans

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  1. Lindsay V. Clark
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Correspondence to Lindsay V. Clark.

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10530_2012_369_MOESM1_ESM.pdf

Online Resource 1 Supplementary tables and figures. Detailed information is provided regarding sampling sites, accessions, ploidy, chloroplast data, and genetic clustering. Supplementary material 1 (PDF 583 kb)

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Clark, L.V., Evans, K.J. & Jasieniuk, M. Origins and distribution of invasive Rubus fruticosus L. agg. (Rosaceae) clones in the Western United States. Biol Invasions 15, 1331–1342 (2013). https://doi.org/10.1007/s10530-012-0369-8

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