Abstract
This experiment was carried out to investigate the potential probiotics properties of Bacillus subtilis in protecting of juvenile freshwater prawn, Macrobrachium rosenbergii against Aeromonas hydrophila infection. A B. subtilis bacterium isolated from gut of juvenile prawns was added to the prawns feed at 108 cells g−1 feed. There were significant differences (P < 0.05) between B. subtilis-treated and control groups in growth and survival enhancement of juvenile prawns after 60 days of feeding trial. Sixty days after B. subtilis feeding trial, the prawns were challenged by bath exposure to A. hydrophila (107 cells mL−1) for 28 days. Four weeks after challenge, there was significant difference in the survival of prawns between B. subtilis-treated groups (88.33 %) and control groups (20.81 %; P < 0.05). In addition, the control groups had an unhealthy external appearance, while the treated groups, appeared healthy and normal. From this, it was concluded that the selected B. subtilis may be a promising probiotics for protection of prawns from A. hydrophila infection.
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The authors would like to thank the staff of Aquaculture Laboratory and Hatchery unit in Bushehr Persian Gulf Jihad Agriculture Higher Education Center for use of equipments, their financial and technical supports.
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Keysami, M.A., Mohammadpour, M. Effect of Bacillus subtilis on Aeromonas hydrophila infection resistance in juvenile freshwater prawn, Macrobrachium rosenbergii (de Man). Aquacult Int 21, 553–562 (2013). https://doi.org/10.1007/s10499-012-9588-3
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DOI: https://doi.org/10.1007/s10499-012-9588-3