Skip to main content
SpringerLink
Log in

Trichoderma mienum sp. nov., isolated from mushroom farms in Japan

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

During an investigation of Hypocrea/Trichoderma species inhabiting mushroom bedlogs, we found five strains of an undescribed species from a culture collection. These were analyzed using a combined approach, including morphology of holomorph, cultural studies, and phylogenetic analyses of the rRNA gene cluster of the internal transcribed spacer region, translation elongation factor 1-α, and RNA polymerase subunit II gene sequences. Distinctive morphological characters include stromata with green ascospores produced on potato dextrose agar medium, and Gliocladium-like to irregularly Verticillium-like conidiophores. In phylogenetic analyses, this species belongs to the Semiorbis clade, but its morphological characteristics do not match the other members of this clade. Based on morphological observations and phylogenetic analyses, we describe this as a new species, Trichoderma mienum, representing its Hypocrea teleomorph and Trichoderma anamorph.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556

    Article  CAS  Google Scholar 

  • Chaverri P, Samuels GJ (2003) Hypocrea/Trichoderma (Ascomycota, Hypocreales, Hypocreaceae): species with green ascospores. Stud Mycol 48:1–116

    Google Scholar 

  • Chaverri P, Samuels GJ, Stewart EL (2001) Hypocrea virens sp. nov., the teleomorph of Trichoderma virens. Mycologia 93:1113–1124

    Article  CAS  Google Scholar 

  • Chaverri P, Castlebury LA, Overton BE, Samuels GJ (2003) Hypocrea/Trichoderma: species with conidiophore elongations and green conidia. Mycologia 95:1100–1140

    Article  PubMed  Google Scholar 

  • Chun J (1995) Computer-assisted classification and identification of Actinomycetes. Doctoral Thesis, University of Newcastle

  • Cubero OF, Crespo ANA, Fatehi F, Bridge PD (1999) DNA extraction and PCR amplication method suitable for fresh, herbarium-stored, lichenized, and other fungi. Plant Syst Evol 216:243–249

    Article  CAS  Google Scholar 

  • Degenkolb T, Dieckmann R, Nielsen KF, Gräfenhan T, Theis C, Zafari D, Chaverri P, Ismaiel A, Brückner H, von Döhren H, Thrane U, Petrini O, Samuels GJ (2008a) The Trichoderma brevicompactum clade: a separate lineage with new species, new peptaibiotics, and mycotoxins. Mycol Prog 7:177–219

    Article  Google Scholar 

  • Degenkolb T, von Döhren H, Nielsen KF, Samuels GJ, Brückner H (2008b) Recent advances and future prospects in peptaibiotics, hydrophobin, and mycotoxin research, and their importance for chemotaxonomy of Trichoderma and Hypocrea. Chem Biodivers 5:671–680

    Article  PubMed  CAS  Google Scholar 

  • Dingley JM (1952) The Hypocreales of New Zealand. III: the genus Hypocrea. Trans Proc R Soc N Z 79:323–337

    Google Scholar 

  • Dingley JM (1957) Life history studies in the genus Hypocrea Fr. Trans Proc R Soc N Z 84:689–693

    Google Scholar 

  • Dodd SL, Lieckfeldt E, Samuels GJ (2003) Hypocrea atroviridis sp. nov., the teleomorph of Trichoderma atroviride. Mycologia 95:27–40

    Article  PubMed  Google Scholar 

  • Doi Y (1972) Revision of the Hypocreales with cultural observations. IV. The genus Hypocrea and its allies in Japan (2). Enumeration of the species. Bull Natl Sci Mus Tokyo 15:649–751

    Google Scholar 

  • Druzhinina IS, Chaverri P, Fallah P, Kubicek CP, Samuels GJ (2004) Hypocrea flaviconidia, a new species from Costa Rica with yellow conidia. Stud Mycol 50:401–407

    Google Scholar 

  • Druzhinina IS, Kopchinskiy AG, Komon M, Bissett J, Szakacs G, Kubicek CP (2005) An oligonucleotide barcode for species identification on Trichoderma and Hypocrea. Fungal Genet Biol 42:813–828

    Article  PubMed  CAS  Google Scholar 

  • Druzhinina IS, Seidl-Seiboth V, Herrera-Estrella A, Horwitz BA, Kenerley CM, Monte E, Mukherjee PK, Zeilinger S, Grigoriev IV, Kubicek CP (2011) Trichoderma: the genomics of opportunistic success. Nat Rev Microbiol 9:749–759

    Article  PubMed  CAS  Google Scholar 

  • Froslev TG, Matheny PB, Hibbett DS (2005) Lower level relationships in the mushroom genus Cortinarius (Basidiomycota, Agaricales): a comparison of RPB1, RPB2, and ITS phylogenies. Mol Phylogenet Evol 37:602–618

    Article  PubMed  CAS  Google Scholar 

  • Hawksworth DL (2012) Managing and coping with names of pleomorphic fungi in a period of transition. IMA Fungus 3:15–24

    Article  Google Scholar 

  • Jaklitsch WM (2009) European species of Hypocrea Part I. The green-spored species. Stud Mycol 63:1–91

    Article  PubMed  Google Scholar 

  • Jaklitsch WM (2011) European species of Hypocrea Part II: species with hyaline ascospores. Fungal Divers 48:1–250

    Article  PubMed  Google Scholar 

  • Jaklitsch WM, Komon M, Kubicek CP, Druzhinina IS (2005) Hypocrea voglmayrii sp. nov. from the Austrian Alps represents a new phylogenetic clade in Hypocrea/Trichoderma. Mycologia 97:1365–1378

    Article  PubMed  CAS  Google Scholar 

  • Kim CS, Park MS, Kim SC, Maekawa N, Yu SH (2012) Identification of Trichoderma, a competitor of shiitake mushroom (Lentinula edodes), and competition between Lentinula edodes and Trichoderma species in Korea. Plant Pathol J (in press)

  • Komatsu M (1976) Studies on Hypocrea, Trichoderma and allied fungi antagonistic to shiitake, Lentinus edodes (Berk.) Sing (in Japanese). Report of the Tottori Mycological Institute no. 13. Japan

  • Kopchinskiy A, Komoń M, Kubicek CP, Druzhinina IS (2005) TrichoBLAST: a multiloci database of phylogenetic markers for Trichoderma and Hypocrea powered by sequence diagnosis and similarity search tools. Mycol Res 109:658–660

    Article  PubMed  Google Scholar 

  • Liu YJ, Whelen S, Hall BD (1999) Phylogenetic relationships among Ascomycetes: evidence from a RNA polymerase II subunit. Mol Biol Evol 16:1799–1808

    Article  PubMed  CAS  Google Scholar 

  • Lu BS, Druzhinina IS, Fallah P, Chaverri P, Gradinger C, Kubicek CP, Samuels GJ (2004) Hypocrea/Trichoderma species with Pachybasium-like conidiophores: teleomorphs for T. minutisporum and T. polysporum and their newly discovered relatives. Mycologia 96:310–342

    Article  PubMed  Google Scholar 

  • Miyazaki K, Tsunoda M (2003) Application of DNA markers to research on Trichoderma in mushroom facilities of Japan (1): RAPD, SSCP marker. Mushroom Sci Biotechnol 11:65–70

    Google Scholar 

  • Miyazaki K, Tsuchiya Y, Okuda T (2009) Specific PCR assays for the detection of Trichoderma harzianum causing green mold disease during mushroom cultivation. Mycoscience 50:94–99

    Article  CAS  Google Scholar 

  • Nirenberg HI (1976) Untersuchungen über die morphologische und biologische Differnzierung in der Fusarium-Sektion Liseola. Mitteilungen aus der Biologischen Bundesanstalt für Land- und Forstwirtschaft Berlin-Dahlem 169:1–117

    Google Scholar 

  • Overton BE, Stewart EL, Geiser DM, Jaklitsch WM (2006) Systematics of Hypocrea citrina and related taxa. Stud Mycol 56:1–38

    Article  PubMed  Google Scholar 

  • Park MS, Seo GS, Lee KH, Bae KS, Yu SH (2005) Morphological and cultural characteristics of Trichoderma spp. associated with green mold of oyster mushroom in Korea. Plant Pathol J 21:221–228

    Article  Google Scholar 

  • Park MS, Bae KS, Yu SH (2006) Two new species of Trichoderma associated with green mold of oyster mushroom cultivation in Korea. Mycobiology 34(3):111–113

    Article  Google Scholar 

  • Põldmaa K (2011) Tropical species of Cladobotryum and Hypomyces producing red pigments. Stud Mycol 68:1–34

    Article  PubMed  Google Scholar 

  • Posada D (2008) jModelTest: phylogenetic model averaging. Mol Biol Evol 25:1253–1256

    Article  PubMed  CAS  Google Scholar 

  • Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574

    Article  PubMed  CAS  Google Scholar 

  • Samuels GJ (2006) Trichoderma: systematic, the sexual state and ecology. Phytopathology 96:195–206

    Article  PubMed  CAS  Google Scholar 

  • Samuels GJ, Ismaiel A (2011) Hypocrea peltata: a mycological Dr Jekyll and Mr Hyde? Mycologia 103:616–630

    Article  PubMed  Google Scholar 

  • Samuels GJ, Dodd SL, Gams W, Castlebury LA, Petrini O (2002) Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia 94:146–168

    Article  PubMed  Google Scholar 

  • Swofford DL (2002) PAUP. Phylogenetic analysis using parsimony, version 4.0. Sinauer Associates, Sunderland

    Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) ClustalX: windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4878

    Article  PubMed  CAS  Google Scholar 

  • Tokimoto K (1985) Physiological studies on antagonism between Lentinus edodes and Trichoderma spp. in bedlogs of the former (in Japanese). Report of the Tottori Mycological Institute no. 23. Japan

  • Watanabe S, Kumakura K, Kato H, Iyozumi H, Togawa M, Nagayama K (2005) Identification of Trichoderma SKT-1, a biological control agent against seedborne pathogens of rice. J Gen Plant Pathol 71:351–356

    Article  CAS  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ et al (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  • Zhang C, Liu S, Lin F, Kubicek CP, Druzhinina IS (2007) Trichoderma taxi sp. nov., an endophytic fungus from Chinese yew Taxus mairei. FEMS Microbiol Lett 251:251–257

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by the Global Center of Excellence (COE) Program, “Advanced Utilization of Fungus/Mushroom Resources for Sustainable Society in Harmony with Nature”, from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.

Author information

Authors and Affiliations

  1. The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-cho-minami, Tottori, 680-8553, Japan

    Chang Sun Kim

  2. Fungus/Mushroom Resource and Research Center, Tottori University, 4-101 Koyama-cho-minami, Tottori, 680-8553, Japan

    Takashi Shirouzu, Akira Nakagiri, Kozue Sotome & Nitaro Maekawa

  3. The Tottori Mycological Institute, 211 Kokoge, Tottori, 689-1125, Japan

    Eiji Nagasawa

Authors
  1. Chang Sun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takashi Shirouzu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Akira Nakagiri
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kozue Sotome
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Eiji Nagasawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Nitaro Maekawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nitaro Maekawa.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PPTX 121 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, C.S., Shirouzu, T., Nakagiri, A. et al. Trichoderma mienum sp. nov., isolated from mushroom farms in Japan. Antonie van Leeuwenhoek 102, 629–641 (2012). https://doi.org/10.1007/s10482-012-9758-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-012-9758-3

Keywords

Search

Navigation