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Isolation of Vibrio alginolyticus and Vibrio splendidus from captive-bred seahorses with disease symptoms

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Abstract

Vibrio species isolated from diseased seahorses were characterized by PCR amplification of repetitive bacterial DNA elements (rep-PCR) and identified by 16S ribosomal RNA gene sequence analysis. The results demonstrated that Vibrio alginolyticus and Vibrio splendidus were predominant in the lesions of these seahorses. To our knowledge, this is the first time that these bacterial species have been associated with disease symptoms in captive-bred seahorses.

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Acknowledgments

The study was financed by the Spanish Ministry of Science and Technology (CGL2005-05927-C03-01), as part of a coordinated research project (Proyecto Hippocampus; 2005/PC091). Funding was also partially provided by the Regional Government of Galicia (Xunta de Galicia: PGIDIT06PXIC402106PN). We thank P. Quintas and A. Chamorro for their kind assistance in seahorse collection and maintenance.

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Correspondence to José L. Balcázar.

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Balcázar, J.L., Gallo-Bueno, A., Planas, M. et al. Isolation of Vibrio alginolyticus and Vibrio splendidus from captive-bred seahorses with disease symptoms. Antonie van Leeuwenhoek 97, 207–210 (2010). https://doi.org/10.1007/s10482-009-9398-4

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