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Characterization and distribution of tomato yellow margin leaf curl virus, a begomovirus from Venezuela

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Abstract

A begomovirus causing mottling and leaf deformation in tomato from the State of Mérida was cloned and sequenced. The virus has a bipartite genome comprised of a DNA-A (2,572 nucleotides) and a DNA-B (2,543 nucleotides) with a genome organization typical of New World begomoviruses. Both components share a common region of 115 nucleotides with 98 % sequence identity. Phylogenetic analysis indicated that while no virus sequences were closely related, the A component was distantly related to those of two other tomato-infecting viruses, tomato leaf deformation virus and Merremia mosaic virus; and the DNA-B, to those of pepper huasteco yellow vein virus and Rhynchosia golden mosaic Yucatan virus. The DNA-A and DNA-B sequences were submitted to GenBank (accession no. AY508993 and AY508994, respectively) and later accepted by the International Committee on Taxonomy of Viruses as the genome of a member of a unique virus species with the name Tomato yellow margin leaf curl virus (TYMLCV). Tomato (Solanum lycopersicum L. ‘Fl. Lanai’) plants inoculated with cloned TYMLCV DNA-A and DNA-B became systemically infected and showed chlorotic margins and leaf curling. The distribution of TYMLCV in tomato-producing states in Venezuela was determined by nucleic acid spot hybridization analysis of 334 tomato leaf samples collected from ten states using a TYMLCV-specific probe and confirmed by PCR and sequencing of the PCR fragment. TYMLCV was detected in samples from the states of Aragua, Guárico, and Mérida, suggesting that TYMLCV is widely distributed in Venezuela.

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Acknowledgements

The authors would like to acknowledge the support of A. Londoño through an Innovation Fund Grant provided by the University of Florida, Office of the Dean for Research, and to S. Duffy for critical reviews of the manuscript.

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Author notes

  1. A. Nava

    Present address: Departamento de Agronomía, Facultad de Agronomía, La Universidad del Zulia, AP, 15205, Maracaibo, Venezuela

Authors and Affiliations

  1. Department of Plant Pathology, University of Florida, Gainesville, FL, 32611, USA

    A. Nava, A. Londoño & J. E. Polston

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  1. A. Nava
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  2. A. Londoño
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Correspondence to J. E. Polston.

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Supplementary material 1 (DOC 112 kb)

705_2012_1501_MOESM2_ESM.tif

Supplementary material 2 Genome organization of tomato yellow margin leaf curl virus (TYMLCV). Location and extent of open reading frames and primers used in the study are noted. (TIFF 146 kb)

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Nava, A., Londoño, A. & Polston, J.E. Characterization and distribution of tomato yellow margin leaf curl virus, a begomovirus from Venezuela. Arch Virol 158, 399–406 (2013). https://doi.org/10.1007/s00705-012-1501-x

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  • DOI: https://doi.org/10.1007/s00705-012-1501-x

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