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CT perfusion-derived mean transit time of cortical brain has a negative correlation with the plasma level of Nitric Oxide after subarachnoid hemorrhage

  • Experimental Research - Brain Injury
  • Published:
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Abstract

Background

Vasospasm of both large and small parenchymal arteries may contribute to the occurrence of delayed ischemic neurological deficits, and nitric oxide(NO) is an important mediators in the development of cerebral vasospasm after subarachnoid hemorrhage (SAH). We used a rabbit two-hemorrhage model to investigate changes in plasma NO after SAH, and the relationship between NO and brain microcirculation.

Methods

SAH was induced in rabbits and a control group was sham operated. There were 32 rabbits in each group that survived the second operation, and they were randomly assigned to four groups of eight rabbits each for follow-up assessments on Days 1, 4, 7, or 14, respectively. Cerebral blood flow (CBF), cerebral blood volume (CBV), and mean transit time (MTT) were calculated at six regions of interest (ROIs): symmetrical areas of the frontal, parietal-occipital, and temporal lobes. Before the contrast CT scan, blood was drawn from the central artery of the ear for measurement of plasma NO.

Results

In the control group, there was no difference in CBV, CBF, and MTT in the six ROIs, and plasma NO was unchanged. Compared to controls, in the SAH group, CBV decreased slightly in the six ROIs (P > 0.05), frontal lobe CBF decreased, MTT increased (P < 0.05, for both), and NO plasma levels were significantly lower (P < 0.01).

Conclusions

There was a significant correlation between the increase in MTT and the decrease in plasma NO (P < 0.05), We hypothesized that normalization of NO might have a positive influence on brain microcirculation following SAH.

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Acknowledgement

This work was supported by grant 81071151 from the National Natural Science Foundation of China.

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None.

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Authors and Affiliations

  1. Departments of Radiology, First Affiliated Hospital of China Medical University, 155 Nanjing N St, Shenyang, 110001, Liaoning Province, People’s Republic of China

    Ruibin Zheng, Lei Qin, Songbai Li, Ke Xu & Haiyang Geng

  2. Key laboratory of Diagnostic Imaging and Interventional Radiology, Shenyang, Liaoning Province, China

    Ruibin Zheng, Lei Qin, Songbai Li, Ke Xu & Haiyang Geng

Authors
  1. Ruibin Zheng
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  2. Lei Qin
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  3. Songbai Li
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  4. Ke Xu
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  5. Haiyang Geng
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Corresponding author

Correspondence to Songbai Li.

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Comment

The present manuscript demonstrated the correlation of cerebral perfusion and a biomarker after subarachnoid hemorrhage in animals.

This study supports the importance of CT-perfusion in aneurysmal SAH.

Daniel Haenggi

Duesseldorf, Germany

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Zheng, R., Qin, L., Li, S. et al. CT perfusion-derived mean transit time of cortical brain has a negative correlation with the plasma level of Nitric Oxide after subarachnoid hemorrhage. Acta Neurochir 156, 527–533 (2014). https://doi.org/10.1007/s00701-013-1968-6

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  • DOI: https://doi.org/10.1007/s00701-013-1968-6

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