Abstract
Cystic echinococcosis (CE) due to the formation of a hydatic cyst is a disease commonly seen in humans and animals that can be mortal sometimes. This disease, which is present in many countries around the world, constitutes a great problem for public health and the economy. The aim of this study was to identify Echinococcus granulosus genotypes in formalin-fixed, paraffin-embedded tissues. Tissue samples from 70 human patients with histologically confirmed echinococcosis were analysed by direct PCR of the 12S rRNA gene and by DNA sequence analysis of the CO1 gene of E. granulosus. Of the 70 samples, 29 (41.6%) could be genotypically characterized. Specifically, 26 of 70 were positive by direct G1-3 PCR of the 12S rRNA gene, two of which were identified as G1 by additional CO1 gene sequencing. All the 44 unidentified samples underwent CO1 sequencing, which yielded one G3 and two G6 records, while the remaining 41 samples gave no or inconclusive results. In conclusion, the results from the analysis of human isolates of E. granulosus confirmed the occurrence of G1, G3 and G6 genotypes in Turkey and indicated G1/G3 cluster (E. granulosus sensu stricto) as the predominant genotype.
Similar content being viewed by others
References
Bowles J, Blair D, McManus DP (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol 54:165–174
Casulli A, Zeyhle E, Brunetti E, Pozio E, Meroni V, Genco F, Filice C (2010) Molecular evidence of the camel strain (G6 genotype) of Echinococcus granulosus in humans from Turkana, Kenya. Trans R Soc Trop Med Hyg 104:29–32
Dinkel A, Njoroge EM, Zimmermann A, Walz M, Zeyhle E, Elhamdi IE, Mackenstdt U, Romig T (2004) A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in Eastern Africa. Int J Parasitol 34:645–653
Ergin S, Saribas S, Yuksel P, Zengin K, Midilli K, Adas G, Arikan S, Aslan M, Uysal H, Caliskan R, Oner A, Kucukbasmaci O, Kaygusuz A, Torun MM, Kocazeybek B (2010) Genotypic characterisation of Echinococcus granulosus isolated from human in Turkey. Afr J Microbiol Res 4:551–555
Kamenetzky L, Canova SG, Guarnera EA, Rosenzvit MC (2000) Echinococcus granulosus: DNA extraction from germinal layers allows strain determination in fertile and nonfertile hydatid cysts. Exp Parasitol 95:122–127
Lavikainen A, Lehtinen MJ, Meri T, Hirvela-Koski V, Meri S (2003) Molecular genetic characterization of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus. Parasitology 127:207–211
Manterola C, Benavente F, Melo A, Vial M, Roa JC (2008) Description of Echinococcus granulosus genotypes in human hydatidosis in a region of southern Chile. Parasitol Int 57:342–346
Moks E, Jõgisalu I, Valdmann H, Saarma U (2008) First report of Echinococcus granulosus G8 in Eurasia and a reappraisal of the phylogenetic relationships of ‘genotypes’ G5-G10. Parasitology 135:647–654
Nakao M, McManus DP, Schantz PM, Craig PS, Ito A (2007) A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitology 134:1–10
Nakao M, Yanagida T, Okamoto M, Knapp J, Nkouawa A, Sako Y, Ito A (2010) State-of-the-art Echinococcus and Taenia: phylogenetic taxonomy of human-pathogenic tapeworms and its application to molecular diagnosis. Infect Genet Evol 10:444–452
Obwaller A, Schneider R, Walochnik J, Gollackner B, Deutz A, Janitschke K, Aspock H, Auer H (2004) Echinococcus granulosus strain differentiation based on sequence heterogeneity in mitochondrial genes of cytochrome c oxidase-1 and NADH dehydrogenase-1. Parasitology 128:569–575
Rozenzvit MC, Zhang LH, Kamenetzky L, Canova SG, Guarnera EA, McManus DP (1999) Genetic variation and epidemiology of Echinococcus granulosus in Argentina. Parasitology 118:523–530
Saarma U, Jogisalu I, Moks E, Varcasia A, Lavikainen A, Oksanen A, Simsek S, Andresiuk V, Denegri G, Gonzalez LM et al (2009) A novel phylogeny for the genus Echinococcus, based on nuclear data, challenges relationships based on mitochondrial evidence. Parasitology 136:317–328
Schneider R, Gollackner B, Edel B, Schmid K, Wrba F, Tucek G, Walochnik J, Auer H (2008) Development of a new PCR protocol for the detection of species and genotypes (strains) of Echinococcus in formalin-fixed, paraffin-embedded tissues. Int J Parasitol 38:1065–1071
Sharbatkhori M, Fasihi Harandi M, Mirhendi H, Hajialilo E, Kia EB (2010) Sequence analysis of cox1 and nad1 genes in Echinococcus granulosus G3 genotype in camels (Camelus dromedarius) from central Iran. Parasitol Res (in press)
Shi SR, Cote RJ, Wu L, Liu C, Datar R, Shi Y, Liu D, Lim H, Taylor CR (2002) DNA extraction from archival formalin-fixed, paraffin-embedded tissue sections based on the antigen retrieval principle: heating under the influence of pH. J Histochem Cytochem 50:1005–1011
Simsek S, Eroksuz Y (2009) Occurrence and molecular characterization of Echinococcus granulosus in Turkish mouflon (Ovis gmelinii anatolica). Acta Trop 109:167–169
Simsek S, Balkaya I, Koroglu E (2010) Epidemiological survey and molecular characterization of Echinococcus granulosus in cattle in an endemic area of eastern Turkey. Vet Parasitol 172:347–349
Snábel V, Altintas N, D'Amelio S, Nakao M, Romig T, Yolasigmaz A, Gunes K, Turk M, Busi M, Hüttner M, Sevcová D, Ito A, Altintas N, Dubinský P (2009) Cystic echinococcosis in Turkey: genetic variability and first record of the pig strain (G7) in the country. Parasitol Res 105:145–154
Thompson RCA (1995) Biology and systematics of Echinococcus. In: Thompson RCA, Lymbery AJ (eds) Echinococcus and hydatid disease. CAB International, Wallingford, pp 1–50
Thompson RCA, Lymbery AJ (1990) Echinococcus: biology and strain variation. Int J Parasitol 20:457–470
Thompson RCA, Lymbery AJ, Constantine CC (1995) Variation in Echinococcus: towards a taxonomic revision of the genus. Adv Parasitol 35:145–176
Thompson RCA, Boxell AC, Ralston BJ, Constantine CC, Hobbs RP, Shury T, Olson ME (2006) Molecular and morphological characterization of Echinococcus in cervids from North America. Parasitology 132:439–447
Utuk AE, Simsek S, Koroglu E, McManus DP (2008) Molecular genetic characterization of different isolates of Echinococcus granulosus in east and southeast regions of Turkey. Acta Trop 107:192–194
Vural G, Baca AU, Gauci CG, Bagci O, Gicik Y, Lightowlers MW (2008) Variability in the Echinococcus granulosus cytochrome C oxidase 1 mitochondrial gene sequence from livestock in Turkey and a re-appraisal of the G1-3 genotype cluster. Vet Parasitol 154:347–350
Acknowledgement
This study was supported by a grant from Firat University Research Foundation (project no. 1749). We thank Dr. Georgios Theodoropoulos for his excellent help in language editing.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Simsek, S., Kaplan, M. & Ozercan, I.H. A comprehensive molecular survey of Echinococcus granulosus in formalin-fixed paraffin-embedded tissues in human isolates in Turkey. Parasitol Res 109, 411–416 (2011). https://doi.org/10.1007/s00436-011-2269-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-011-2269-8