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Evaluation of the Potential Antimicrobial Resistance Transfer from a Multi-Drug Resistant Escherichia coli to Salmonella in Dairy Calves

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Abstract

Previous research conducted in our laboratory found a significant prevalence of multi-drug resistant (MDR) Salmonella and MDR Escherichia coli (MDR EC) in dairy calves and suggests that the MDR EC population may be an important reservoir for resistance elements that could potentially transfer to Salmonella. Therefore, the objective of the current research was to determine if resistance transfers from MDR EC to susceptible strains of inoculated Salmonella. The experiment utilized Holstein calves (approximately 3 weeks old) naturally colonized with MDR EC and fecal culture negative for Salmonella. Fecal samples were collected for culture of Salmonella and MDR EC throughout the experiment following experimental inoculation with the susceptible Salmonella strains. Results initially suggested that resistance did transfer from the MDR E. coli to the inoculated strains of Salmonella, with these stains demonstrating resistance to multiple antibiotics following in vivo exposure to MDR EC. However, serogrouping and serotyping results from a portion of the Salmonella isolates recovered from the calves post-challenge, identified two new strains of Salmonella; therefore transfer of resistance was not demonstrated under these experimental conditions.

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Acknowledgments

This research was funded in part by beef and veal producers and importers through their $1-per-head checkoff and was produced for the Cattlemen’s Beef Board and state beef councils by the National Cattlemen’s Beef Association

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Authors and Affiliations

  1. Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, USDA-ARS, 2881 F&B Road, College Station, TX, 77845, USA

    T. S. Edrington, R. L. Farrow, M. E. Hume, T. R. Callaway, R. C. Anderson & D. J. Nisbet

  2. Poultry Science Department, Texas A&M University, College Station, TX, 77845, USA

    P. N. Anderson & D. J. Caldwell

  3. Agricultural Experiment Station, New Mexico State University, Clovis, NM, 88101, USA

    G. R. Hagevoort

Authors
  1. T. S. Edrington
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  2. R. L. Farrow
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  3. M. E. Hume
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  4. P. N. Anderson
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  5. G. R. Hagevoort
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  6. D. J. Caldwell
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  7. T. R. Callaway
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  8. R. C. Anderson
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  9. D. J. Nisbet
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Corresponding author

Correspondence to T. S. Edrington.

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Edrington, T.S., Farrow, R.L., Hume, M.E. et al. Evaluation of the Potential Antimicrobial Resistance Transfer from a Multi-Drug Resistant Escherichia coli to Salmonella in Dairy Calves. Curr Microbiol 66, 132–137 (2013). https://doi.org/10.1007/s00284-012-0249-6

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  • DOI: https://doi.org/10.1007/s00284-012-0249-6

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