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Influence of Lipopolysaccharide on the Surface Proton-Binding Behavior of Shewanella spp.

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Abstract

This study investigates the potentiometric properties of several strains of Shewanella spp. and determines whether these properties can be correlated with lipopolysaccharide (LPS) type. The LPS of eight Shewanella strains was characterized using silver-stained sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and their potentiometric properties determined using high-resolution acid–base titrations. Titrations showed that total ligand concentrations (L T ) ranged from 0.903 ± 0.007 μmol/mg (S. baltica 63) to 1.387 ± 0.007 μmol/mg (S. amazonensis SB2B). Smooth strains (possessing O-side chains) exhibited higher mean L T values than rough strains (no O-side chain). A Tukey’s honestly significantly different (HSD) test revealed, smooth strains exhibited significantly higher L T values than rough strains in 69% of comparisons. Comparison of individual pK a concentrations revealed that smooth LPS strains of Shewanella were relatively enriched in reactive groups at pK a 5, suggesting their LPS O-side chains contained detectable carboxyl groups. Combined pKa spectra from all eight Shewanella strains produced a common trend indicating that the way in which the surface proton-buffering capacity changes with pH is similar for the species studied here.

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Acknowledgments

This work was supported by Natural Science and Engineering Research Council (NSERC) of Canada Discovery, US-DOE-NABIR, and Advanced Food & Materials Network–National Centres for Excellence grants to TJB and through an Ontario Premier’s Research Excellence Award to FGF.

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Authors and Affiliations

  1. Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph, Guelph, N1G 2W1, Ontario, Canada

    Vernon R. Phoenix, Anton A. Korenevsky & T. J. Beveridge

  2. Department of Geology, University of Toronto, 22 Russell Street, M5S 3B1, Ontario, Canada

    Vernon R. Phoenix & F. G. Ferris

  3. Department of Geographical and Earth Sciences, University of Glasgow, Glasgow, UK

    Vernon R. Phoenix

  4. Pacific Northwest National Laboratories, Richland, Washington, WA, 99352, USA

    Y. A. Gorby

  5. J. Craig Venter Institute, 11149 North Torrey Pines Road, Suite 200, La Jolla, CA, 92037, USA

    Y. A. Gorby

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  1. Vernon R. Phoenix
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  2. Anton A. Korenevsky
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  3. F. G. Ferris
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  4. Y. A. Gorby
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  5. T. J. Beveridge
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Correspondence to Vernon R. Phoenix.

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Phoenix, V.R., Korenevsky, A.A., Ferris, F.G. et al. Influence of Lipopolysaccharide on the Surface Proton-Binding Behavior of Shewanella spp.. Curr Microbiol 55, 152–157 (2007). https://doi.org/10.1007/s00284-007-0077-2

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  • DOI: https://doi.org/10.1007/s00284-007-0077-2

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