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Genomic regions associated with the nitrogen limitation response revealed in a global wheat core collection

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Abstract

Modern wheat (Triticum aestivum L.) varieties in Western Europe have mainly been bred, and selected in conditions where high levels of nitrogen-rich fertilizer are applied. However, high input crop management has greatly increased the risk of nitrates leaching into groundwater with negative impacts on the environment. To investigate wheat nitrogen tolerance characteristics that could be adapted to low input crop management, we supplied 196 accessions of a wheat core collection of old and modern cultivars with high or moderate amounts of nitrogen fertilizer in an experimental network consisting of three sites and 2 years. The main breeding traits were assessed including grain yield and grain protein content. The response to nitrogen level was estimated for grain yield and grain number per m2 using both the difference and the ratio between performance at the two input levels and the slope of joint regression. A large variability was observed for all the traits studied and the response to nitrogen level. Whole genome association mapping was carried out using 899 molecular markers taking into account the five ancestral group structure of the collection. We identified 54 main regions involving almost all chromosomes that influence yield and its components, plant height, heading date and grain protein concentration. Twenty-three regions, including several genes, spread over 16 chromosomes were involved in the response to nitrogen level. These chromosomal regions may be good candidates to be used in breeding programs to improve the performance of wheat varieties at moderate nitrogen input levels.

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Acknowledgments

The experimental work was partly supported by the Enterprise Competitiveness Fund Project “Semences de Demain”.

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Authors and Affiliations

  1. INRA, UMR 1095 Génétique, Diversité Et Ecophysiologie Des Céréales, 234 Avenue Du Brézet, 63100, Clermont-Ferrand, France

    Jacques Bordes, C. Ravel, G. Charmet, J. Le Gouis & F. Balfourier

  2. Université Blaise Pascal, UMR 1095 Génétique, Diversité Et Ecophysiologie Des Céréales, 63177, Aubière Cedex, France

    Jacques Bordes, C. Ravel, G. Charmet, J. Le Gouis & F. Balfourier

  3. Agri Obtentions Chemin de La Petite, Minière, Guyancourt, 78280, France

    J. P. Jaubertie & A. L. Pissavy

  4. Limagrain Europe, BP3, 77390, Verneuil l’Etang, France

    B. Duperrier

  5. INRA-UPS-CNRS-AgroParisTech, UMR de Génétique Végétale, Ferme Du Moulon, 91190, Gif-sur-Yvette, France

    O. Gardet

  6. INRA, UMR 1281 Stress Abiotiques Et Différenciation Des Végétaux Cultivés, Estrées-Mons, 80203, Péronne, France

    E. Heumez

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  1. Jacques Bordes
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  2. C. Ravel
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Correspondence to Jacques Bordes.

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Communicated by A. Graner.

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Bordes, J., Ravel, C., Jaubertie, J.P. et al. Genomic regions associated with the nitrogen limitation response revealed in a global wheat core collection. Theor Appl Genet 126, 805–822 (2013). https://doi.org/10.1007/s00122-012-2019-z

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