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Measurement of polyunsaturated fatty acids of myocardial lipids by high performance liquid chromatography

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Summary

This report describes a modified method for the separation and analysis of polyunsaturated fatty acids such as 20:4, 20:5, and 22:6, using HPLC. The results show that these fatty acids are well separated from the saturated acids. Since the unsaturated fatty acids elute earlier than saturated acids, and this method does not require the fractionation of free fatty acids using thin layer chromatography, a necessary step for the gas chromatographic analysis, the recoveries of polyunsaturated fatty acids were significantly higher as compared to those from gas chromatography. Furthermore, HPLC and gas chromatographic methods gave identical results for the acyl chain composition of phosphatidylserine. The advantages of using HPLC over gas chromatography in determining the acyl chain composition of free fatty acids and phospholipids are also discussed.

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Authors and Affiliations

  1. Department of Surgery, University of Connecticut School of Medicine, 06032, Farmington, CT, USA

    G. A. Cordis, M. Renuka Prasad, H. Otani & D. K. Das

  2. Baystate Medical Center, 01107, Springfield, MA, USA

    R. M. Engelman

Authors
  1. G. A. Cordis
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  2. M. Renuka Prasad
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  3. H. Otani
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  4. R. M. Engelman
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  5. D. K. Das
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Cordis, G.A., Renuka Prasad, M., Otani, H. et al. Measurement of polyunsaturated fatty acids of myocardial lipids by high performance liquid chromatography. Chromatographia 24, 687–690 (1987). https://doi.org/10.1007/BF02688569

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  • DOI: https://doi.org/10.1007/BF02688569

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