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Detection of anaerobic mycoplasmas in cell cultures

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Summary

A commercially available anaerobic generator and incubation system that develops a low oxidation-reduction potential was used for the assay of cell cultures for mycoplasmal contamination. Mycoplasma broth and agar media supplemented with dextrose, yeast extract, and horse serum were used. This system supported growth of some mycoplasmas that failed to grow in incubators with 5% CO2 in nitrogen previously used in culture of mycoplasmas in this laboratory.

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Authors and Affiliations

  1. Department of Microbiology, Institute for Medical Research, Copewood Street, 08103, Camden, New Jersey

    Gerard J. McGarrity & Lewis L. Coriell

Authors
  1. Gerard J. McGarrity
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  2. Lewis L. Coriell
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Additional information

This work was supported by a grant from the John A. Hartford Foundation General Research Support Grant No. 5 SO1 RR05582-4 from the National Institutes of Health, and by a Grant-in-Aid Contract from the State of New Jersey.

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McGarrity, G.J., Coriell, L.L. Detection of anaerobic mycoplasmas in cell cultures. In Vitro 9, 17–18 (1973). https://doi.org/10.1007/BF02615983

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  • DOI: https://doi.org/10.1007/BF02615983

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