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Analysis of chloroplast and mitochondrial DNA in asymmetric somatic hybrids betweenSolanum tuberosum and irradiatedS. brevidens

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Abstract

Organellar DNA of asymmetric somatic hybrids betweenSolanum tuberosum and irradiatedS. brevidens were analysed by DNA hybridization methods using the spinach chloroplast probepSBD, wheat mitochondrial genenad5 and petunia mitochondrial geneorf25. Eight of the 12 asymmetric hybrid plants hadS. tuberosum chloroplast DNA and the remaining fourS. brevidens chloroplast DNA. A novel mitochondrial hybridization pattern was present in eight out of the 17 hybrids tested. In six hybrids, novel combinations of chloroplasts and mitochondria were found, indicating that both organelle types sorted out independently.

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References

  • Alt, J., Morris, J., Westhoff, P. and Herrmann, R.G. (1984) Nucleotide sequence of the clustered genes for the 44 kD chlorophyll a apoprotein and the ‘32 kD’-like protein of the photosystem II reaction center in the spinach plastid chromosome.Curr. Genet. 8, 597–606.

    CAS  Google Scholar 

  • Austin, S., Baer, M.A. and Helgeson, J.P. (1985) Transfer of resistance to potato leaf roll virus fromSolanum brevidens intoSolanum tuberosum by somatic fusion.Pl. Sci. 39, 75–82.

    Google Scholar 

  • Austin, S., Lojowska, E., Ehlenfeldt, M., Kelman, A. and Helgeson, J.P. (1988) Fertile interspecific somatic hybrids ofSolanum: a novel source of resistance toErwinia soft rot.Phytopathology 78, 1216–20.

    Google Scholar 

  • Barsby, T., Shepard, J., Kemble, R. and Wong, R. (1984) Somatic hybridization in the genusSolanum: S. tuberosum andS. brevidens. Pl. Cell Rep.3, 165–7.

    Google Scholar 

  • Bates, G.W. (1992) Molecular analysis of nuclear genes in somatic hybrids.Physiologia Plantarum 85, 308–14.

    Article  CAS  Google Scholar 

  • Binding, H., Jain, S.M., Finger, J., Mordhorst, G., Nehls, R. and Gressel, J. (1982) Somatic hybridization of an atrazine resistant biotype ofSolanum nigrum withSolanum tuberosum.Theor. Appl. Genet. 63, 273–7.

    Article  Google Scholar 

  • Cardi, T., D'Ambrosio, F., Puite, K.J., Ramulu, K.S. and Consoli, D. (1991) Characterization of plants regenerated from electrofusedSolanum commersonii andS. tuberosum protoplasts.Physiol Plant 82, A24.

    Google Scholar 

  • Derks, F.H.M., Wijbrandi, J., Koornneef, M. and Colijn-Hooymans, C.M. (1991) Organelle analysis of symmetric and asymmetric hybrids betweenLycopersicon peruvianum andLycopersicon esculentum.Theor. Appl. Genet. 81, 199–204.

    Article  Google Scholar 

  • Derks, F.H.M., Hakkert, J.C., Verbeek, W.H.J. and Colijn-Hooymans, C.M. (1992) Genome composition of asymmetric hybrids in relation to the phylogenetic distance between the parents. Nucleus-chloroplast interaction.Theor. Appl. Genet. 84, 930–40.

    Article  Google Scholar 

  • Fehér, A., Freiszner, J., Litkey, Z., Csanádi, G. and Dudits, D. (1992) Characterization of chromosome instability in interspecific somatic hybrids obtained by X-ray fusion between potato (Solanum tuberosum L.) andS. brevidens Phil.Theor. Appl. Genet. 84, 880–90.

    Article  Google Scholar 

  • Fish, N., Karp, A. and Jones, M.G.K. (1988) Production of somatic hybrids by electrofusion inSolanum.Theor. Appl. Genet. 76, 260–6.

    Google Scholar 

  • Fox, T.D. and Leaver, C.J. (1981) TheZea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TCA codons.Cell 26, 315–23.

    Article  CAS  PubMed  Google Scholar 

  • Galun, E. and Aviv, D. (1983) In: Evans, D.A., Sharp, W.R., Ammirato P.V. and Yamada Y. (eds)Techniques for Propagation and Breeding. Handbook of Plant Cell Culture. Vol. 1 pp. 358–89. New York: Macmillan.

    Google Scholar 

  • Isaac, P.G., Jones, V.P., and Leaver, C.J. (1985) The maize cytochrome coxidase subunit I gene: sequence, expression and rearrangement in cytoplasmic male sterile plants.EMBO J 4, 1617–23.

    CAS  PubMed  Google Scholar 

  • Jones, M.G.K. (1988) Fusing plant protoplasts.Bio/Technology 6, 153–8.

    Google Scholar 

  • Kemble, R.J., Barsby, T.L., Wong, R.S.C. and Shepard, J.F. (1986) Mitochondrial DNA rearrangements in somatic hybrids ofSolanum tuberosum andSolanum brevidens.Theor. Appl. Genet. 72, 787–93.

    Article  CAS  Google Scholar 

  • Kumar, A. and Cocking, E.C. (1987) Protoplast fusion: a novel approach to organelle genetics in higher plants.Am. J. Bot. 74, 1289–303.

    Google Scholar 

  • Kushnir, S., Babiychuk, E., Bannikova, M., Momot, V., Komarnitsky, I., Cherep, N. and Gleba, Y. (1991) Nucleo-cytoplasmic incompatibility in cybrid plants possessing anAtropa genome and aNicotiana plastome.Mol. Gen. Genet. 225, 225–30.

    Article  CAS  PubMed  Google Scholar 

  • Pehu, E., Karp, A., Moore, K., Steele, S., Dunckley, R. and Jones, M.G.K. (1989) Molecular, cytogenetic and morphological characterization of somatic hybrids of dihaploidSolanum tuberosum and diploidS. brevidens.Theor. Appl. Genet. 78, 696–704.

    Article  CAS  Google Scholar 

  • Perl, D., Aviv D. and Galun, E. (1990) Protoplast-fusionderivedSolanum cybrids: application and phylogenetic limitations.Theor. Appl. Genet. 79, 632–40.

    Article  CAS  Google Scholar 

  • Puite, K.J., Roest, S. and Pijnacker, L.P. (1986) Somatic hybrid potato plants after electrofusion of diploidSolanum tuberosum andS. phureja.Pl. Cell Rep. 5, 262–5.

    CAS  Google Scholar 

  • Sidorov, V.A. Zubko, M.K., Kuchko, A.A., Komarnitsky, I.K. and Gleba, Y.Y. (1987) Somatic hybridization in potato: use of gamma-irradiated protoplasts ofSolanum pinnatisectum in genetic reconstruction.Theor. Appl. Genet. 74, 364–8.

    Article  Google Scholar 

  • Stamper, S.E., Dewey, R.E., Bland, M.M. and C.S. Levings, III (1987) Characterization of the gene urf13-T and an unidentified reading frame, ORF 25, in maize and tobacco mitochondria.Curr. Genet. 12, 457–63.

    Article  CAS  PubMed  Google Scholar 

  • Xu, Y.S., Murto, M., Dunckley, R., Jones, M.G.K. and Pehu, E. (1993a) Production of asymmetric hybrids betweenSolanum tuberosum and irradiatedS. brevidens. Theor. Appl. Genet. 85, 729–34.

    Google Scholar 

  • Xu, Y.S., Jones, M.G.K., Karp, A. and Pehu, E. (1993b) Analysis of the mitochondrial DNA of the somatic hybrids ofSolanum brevidens andS. tuberosum using non-radioactive digoxigenin-labelled DNA probes.Theor. Appl. Genet. 85, 1017–22.

    CAS  Google Scholar 

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Xu, YS., Pehu, E. Analysis of chloroplast and mitochondrial DNA in asymmetric somatic hybrids betweenSolanum tuberosum and irradiatedS. brevidens . Transgenic Research 3, 256–259 (1994). https://doi.org/10.1007/BF02336779

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  • DOI: https://doi.org/10.1007/BF02336779

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