Summary
To develop a successful protocol forAgrobacterium-mediated transformation in plants it is essential to determine the most efficient bacterial strain/plant genotype interaction. In the present work, we evaluated the susceptibility ofEucalyptus grandis ×E. urophylla to fiveAgrobacterium rhizogenes and twelveA. tumefaciens wildtype strains. The results showed different degrees of virulence, according to the strain tested, indicating that transformation of this eucalypt hybrid by Agrobacterium-derived vectors is possible. All developed tumours showed an autonomous growth when transferred to a hormone-free medium. Some of these tumours formed shoots spontaneously, with a normal phenotype. Polymerase Chain Reaction (PCR) and Southern blot analyses were performed to confirm the absence of the oncogenic T-DNA in plants derived from these shooty tumours.
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Machado, L.d.O.R., de Andrade, G.M., Barrueto Cid, L.P. et al. Agrobacterium strain specificity and shooty tumour formation in eucalypt (Eucalyptus grandis ×E. urophylla). Plant Cell Reports 16, 299–303 (1997). https://doi.org/10.1007/BF01088285
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DOI: https://doi.org/10.1007/BF01088285