Summary
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1.
Intact Ehrlich ascites tumour cells exhibit a residual respiration which is insensitive to site-specific inhibitors of mitochondrial electron transport. Treatment of the tumour-bearing mice with phenobarbital does not influence the rate of this inhibitor-resistant respiration.
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2.
Among the respiratory pigments analysed spectrophotometrically in the microsomal fraction prepared from ascites cells, only small amounts of flavoproteins (NADH-cytochrome b 5 reductase and NADPH-cytochrome c reductase) were detectable.
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3.
Ascites-cell nuclei respire with NADH or NADPH, but not with succinate, as substrates and the respiration is unaffected by inhibitors of mitochondrial electron and energy transfer. These nuclei contain cytochrome b 5, whereas cytochrome P-450 or mitochondrial-like cytochromes are lacking.
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4.
It is concluded that the NAD(P)H-dependent electron transport in nuclei is responsible for the inhibitor-insensitive O2 uptake of intact cells. Conversely, mixed function oxidation reactions of the endoplasmic reticulum have no role in this connection. Moreover, it is assumed that nuclear respiration is involved in the control of the cellular redox potential.
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5.
The relevance of the data obtained with respect to some assumptions concerning the biogenesis of the endoplasmic reticulum is discussed.
Zusammenfassung
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1.
Intakte Ehrlich-Ascites-Tumorzellen zeigen eine Restatmung, die gegenüber locusspeziflschen Inhibitoren des mitochondrialen Elektronentransports unempfindlich sind. Behandlung der tumortragenden Mäuse mit Phenobarbital beeinflußt die Geschwindigkeit dieser inhibitorresistenten Atmung nicht.
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2.
Unter den Atmungspigmenten der Mikrosomenfraktion, die von Ascites-Zellen gewonnen und spektrophotometrisch analysiert wurden, sind nur geringe Mengen Flavoproteine (NADH-Cytochrom-b 5-Reduktase und NADPH-Cytochrom-c-Reduktase) nachweisbar.
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3.
Zellkerne der Ascites-Zellen atmen mit NADH oder NADPH, aber nicht mit Succinat als Substraten. Die Atmung wird von Inhibitoren des mitochondrialen Elektronen- und Energietransports nicht beeinflußt. Diese Kerne enthalten Cytochrom-b 5, während Cytochrom-P-450 oder mitochondrienähnliche Cytochrome fehlen.
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4.
Es wird geschlossen, daß der NAD(P)H-abhängige Elektronentransport in Zellkernen für die inhibitor-unempfindliche Sauerstoffaufnahme intakter Zellen verantwortlich ist. Andererseits spielen mischfunktionelle Oxydationsreaktionen des endoplasmatischen Reticulums in diesem Zusammenhang keine Rolle. Außerdem wird angenommen, daß Kernatmung mit der Kontrolle des cellulären Redox-Potentials zusammenhängt.
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5.
Die Bedeutung der erhaltenen Befunde im Hinblick auf einige Annahmen zur Biogenese des endoplasmatischen Reticulums wird diskutiert.
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Abbreviations
- PB:
-
phenobarbital
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Bartoli, G.M., Dani, A., Galeotti, T. et al. Respiratory activity of Ehrlich ascites tumour cell nuclei. Z. Krebsforsch. 83, 223–231 (1975). https://doi.org/10.1007/BF00304092
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DOI: https://doi.org/10.1007/BF00304092