Summary
Synaptic ribbons (SR) in pinealocytes of adult (120–130 day-old) male Chinese hamsters (Cricetulus griseus) were classified into types 1, 2 and 3; these have a central dense structure showing rod-like, various and ringlike profiles, respectively. The central structure of the type-2 SR usually appeared as round, oval or comma-like bodies, and occasionally as plates showing various profiles or clubshaped bodies. The quantity of each type of SR, expressed as the SR index, was determined over a 24-h period under a light/dark regime (LD) 12∶12 or LD 14∶10. On comparing the results obtained from adults with previously published data from young (60–70-days-old) animals under LD 12∶12, it was found that, in both young and adult animals, the type-1 and type-3 SR indices exhibited different 24-h variations, whereas the type-2 SR index remained constant over a 24-h period. In addition, the indices of the type-2 SR, but not those of the other SR types, were found to be significantly larger in adult than in young animals. In adult animals, the effects of the photoperiod were different between the three types of SR. A nocturnal increase in the type-1 SR index was observed under both LD 12∶12 and LD 14∶10, its time course being different for each of these photoperiods. Under LD 14∶10, the type-2 SR index showed a significant 24-h rhythm with larger values during the dark period; this was not observed under LD 12∶12. The type-3 SR index was almost the same under LD 12∶12 and LD 14∶10. The results suggest that pinealocyte SR of the Chinese hamster may be composed of three types of SR, each with a different functional role.
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References
Abe K, Matsushima S, Mita M (1983) Estimation of the mean size of spheres from the profiles in ultrathin sections: A stereological method. J Electron Microsc 32:57–60
Banks JC, Dalgleish AE, Vollrath L (1985) Postnatal development of “synaptic” ribbons and spherules in the guinea pig pineal gland. Am J Anat 173:43–53
Gaston S, Menaker M (1967) Photoperiodic control of hamster testis. Science 158:925–928
Hewing M (1980) Synaptic ribbons in the pineal system of normal and light deprived golden hamsters. Anat Embryol 159:71–80
Karasek M, Vollrath L (1982) “Synaptic” ribbons and spherules of the rat pineal gland: Day/night changes in vitro? Exp Brain Res 46:205–208
Karasek M, King TS, Richardson BA, Hurlbut EC, Hansen JT, Reiter RJ (1982) Day-night differences in the number of pineal “synaptic” ribbons in two diurnal rodents, the chipmunk (Tamias striatus) and the ground squirrel (Spermophilus richardsonii). Cell Tissue Res 224:689–692
Kosaras B, Welker HA, Mess B, Vollrath L (1983) Depressive effect of LHRH on the numbers of “synaptic” ribbons and spherules in the pineal gland of diestrous rats. Cell Tissue Res 229:461–466
Kurumado K, Mori W (1977) A morphological study of the circadian cycle of the pineal gland of the rat. Cell Tissue Res 182:565–568
Leonhardt H (1967) Über axonähnliche Fortsätze, Sekretbildung und Extrusion der hellen Pinealozyten des Kaninchens. Z Zellforsch 82:307–320
Lues G (1971) Die Feinstruktur der Zirbeldrüse normaler, trächtiger und experimentell beeinflußter Meerschweinchen. Z Zellforsch 114:38–60
Martinez Soriano F, Welker HA, Vollrath L (1984) Correlation of the number of pineal “synaptic” ribbons and spherules with the level of serum melatonin over a 24-hour period in male rabbits. Cell Tissue Res 236:555–560
Matsushima S, Morisawa Y (1978) Effects of acute cold exposure on the ultrastructure of the mouse pinealocyte. Cell Tissue Res 195:461–469
Matsushima S, Morisawa Y, Aida I, Abe K (1983a) Circadian variations in pinealocytes of the Chinese hamster, Cricetulus griseus. A quantitative electron-microscopic study. Cell Tissue Res 228:231–244
Matsushima S, Sakai Y, Aida I (1983b) Effects of melatonin on synaptic ribbons in pinealocytes of the Chinese hamster, Cricetulus griseus. A quantitative electron-microscopic study. Cell Tissue Res 233:59–67
Mikamo K, Kamiguchi Y (1983) A new assessment system for chromosomal mutagenicity using oocytes and early zygotes of the Chinese hamster. In: Ishihara T, Sakaki MS (eds) Radiation-induced chromosome damage in man. Alan R Liss, New York, pp 411–432
Reiter RJ (1983) Melatonin as the hormone which mediates the effects of the pineal gland on neuroendocrine-reproductive axis of the Syrian hamster. In: Axelrod J, Fraschini F, Velo GP (eds) The pineal gland and its endocrine role. Plenum Press, New York London, pp 317–330
Ryan TA (1959) Multiple comparisons in psychological research. Psychol Bull 56:26–47
Ryan TA (1960) Significance tests for multiple comparison of proportions, variances, and other statistics. Psychol Bull 57:318–328
Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Theron JJ, Biagio R, Meyer AC, Boekkooi S (1979) Microfilaments, the smooth endoplasmic reticulum and synaptic ribbon fields in the pinealocytes of the baboon (Papio ursinus). Am J Anat 154:151–162
Vollrath L (1973) Synaptic ribbons of a mammalian pineal gland. Circadian changes. Z Zellforsch 145:171–183
Vollrath L (1981) The pineal organ. In: Oksche A, Vollrath L (eds) Handbuch der mikroskopischen Anatomie des Menschen. Vol. VI/7, Springer, Berlin Heidelberg New York, pp 146–158
Vollrath L, Welker HA (1984) No correlation of pineal “synaptic” ribbon numbers and melatonin formation in individual rat pineal glands. J Pineal Res 1:187–195
Vollrath L, Schultz RL, McMillan PJ (1983) “Synaptic” ribbons and spherules of the guinea pig pineal gland: Inverse day/night differences in number. Am J Anat 168:67–74
Weibel ER, Kistler GS, Scherle WF (1966) Practical stereological methods for morphometric cytology. J Cell Biol 30:23–38
Welsh MG, Cameron IL, Reiter RJ (1979) The pineal gland of the gerbil, Meriones unguiculatus. II. Morphometric analysis over a 24-hour period. Cell Tissue Res 204:95–109
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Aida, I., Sakai, Y., Matsushima, S. et al. A quantitative study of synaptic ribbons in pinealocytes of adult Chinese hamsters (Cricetulus griseus) under different photoperiodic conditions. Cell Tissue Res. 244, 107–113 (1986). https://doi.org/10.1007/BF00218387
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DOI: https://doi.org/10.1007/BF00218387