Abstract
In some cases, human spermatozoa to be used for in vitro fertilization are processed from testicular or epididymal biopsies collected in the clinic or operating room. An appropriately equipped Andrology or Embryology Laboratory is required. Sterility must be maintained at all stages from collection and transport to identification and processing to insemination or cryopreservation. The technologist must be able to properly process and identify spermatozoa from aspirates, seminiferous tubules or pieces of testicular tissue. Recovery of undamaged spermatozoa from tubules or tissue requires mincing, squeezing, or vortexing the tissue, usually without the need of enzymatic digestion. A motility stimulant such as Pentoxifylline is commonly used to calculate the number of functionally competent spermatozoa. After recovery, spermatozoa may be used immediately for IVF-ICSI, incubated overnight prior to IVF-ICSI, or cryopreserved for future use. Methods for identifying, purifying, and determining the number and motility of spermatozoa during these processes are presented.
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References
Schlegel PN (2009) Nonobstructive azoospermia: a revolutionary surgical approach and results. Semin Reprod Med 27:165–170
Schlegel PN, Li PS (1998) Microdissection TESE: sperm retrieval in non-obstructive azoospermia. Hum Reprod Update 4:439
Steele EK et al (2000) Testicular sperm extraction by Trucut needle and milking of seminiferous tubules: a technique with high yield and patient acceptability. Fertil Steril 74:380–383
Okada H et al (2002) Conventional versus microdissection testicular sperm extraction for nonobstructive azoospermia. J Urol 168:1063–1067
Khadra AA et al (2003) Efficiency of percutaneous testicular sperm aspiration as a mode of sperm collection for intracytoplasmic sperm injection in nonobstructive azoospermia. J Urol 169:603–605
Ord T et al (1992) The role of the laboratory in the handling of epididymal sperm for assisted reproductive technologies. Fertil Steril 57:1103–1106
Kamal A et al (2004) Selection of individual testicular tubules from biopsied testicular tissue with a stereomicroscope improves sperm retrieval rate. J Androl 25:123–127
Crabbe E et al (1998) Enzymatic digestion of testicular tissue may rescue the intracytoplasmic sperm injection cycle in some patients with non-obstructive azoospermia. Hum Reprod 13:2791–2796
Aydos K et al (2005) Enzymatic digestion plus mechanical searching improves testicular sperm retrieval in non-obstructive azoospermia cases. Eur J Obstet Gynecol Reprod Biol 120:80–86
Hammitt DG et al (2002) Development of a new and efficient laboratory method for processing testicular sperm. J Assist Reprod Genet 19:335–342
Tokuyama O et al (2003) Using a vortex mixer for testicular sperm collection. J Reprod Med 48:865–868
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Muller, C.H., Pagel, E.R. (2013). Recovery, Isolation, Identification, and Preparation of Spermatozoa from Human Testis. In: Carrell, D., Aston, K. (eds) Spermatogenesis. Methods in Molecular Biology, vol 927. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-038-0_20
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DOI: https://doi.org/10.1007/978-1-62703-038-0_20
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-037-3
Online ISBN: 978-1-62703-038-0
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