Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Allaway D, Schofield NA, Leonard ME, Gilardoni L, Finan TM, Poole PS (2001) Use of differential fluorescence induction and optical trapping to isolate environmentally induced genes. Environ Microbiol 3:397–406
Andersen JB, Sternberg C, Poulsen JK, Bjørn SP, Givskov M, Molin S (1998) New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol 64:2240–2246
Applegate BM, Kehrneyer SR, Sayler GS (1998) A chromosomally based tod-luxCDABE whole-cell reporter for benzene, toluene, ethylbenzene, and xylene (BTEX) sensing. Appl Environ Microbiol 64:2730–2735
Bechor O, Smulski DR, Van Dyk TK, LaRossa RA, Belkin S (2002) Recombinant microorganisms as environmental biosensors: pollutants detection by Escherichia coli bearing fabA’::lux fusions. J Biotechnol 94:125–132
Belkin S, Smulski DR, Vollmer AC, Van Dyk TK, Larossa RA (1996) Oxidative stress detection withEscherichia coli harboring a catG’::lux fusion. Appl Environ Microbiol 62:2252–2256
Belkin S, Smulski DR, Dadon S, Vollmer AC, Van Dyk TR, Larossa RA (1997) A panel of stress-responsive luminous bacteria for the detection of selected classes of toxicants. Water Res 31:3009–3016
BolanosVasquez MC, Warner D (1997) Effects of Rhizobium tropici, R. etli, and R. leguminosarum bv phaseoli on nod gene-inducing flavonoids in root exudates of Phaseolus vulgaris. Mol Plant-Microbe Interact 10:339–346
Boldt T, Sørensen J, Karlson U, Molin S, Ramos C (2004) Combined use of different Gfp reporters for monitoring single-cell activities of a genetically modified PCB degrader in the rhizosphere of alfalfa. FEMS Microbiol Ecol 48:139–148
Boyd EM, Killham K, Wright J, Rumford S, Hetheridge M, Cumming R, Meharg AA (1997) Toxicity assessment of xenobiotic contaminated groundwater using lux modified Pseudomonas fluorescens. Chemosphere 35:1967–1985
Boyd EM, Killham K, Meharg AA (2001) Toxicity of mono-, di-and trichlorophenols to lux marked terrestrial bacteria, Burkholderia species Rasc c2 and Pseudomonas fluorescens. Chemosphere 43:157–166
Brandt KK, Pedersen A, Sørensen J (2002) Solid-phase contact assay that uses a lux-marked Nitrosomonas europaea reporter strain to estimate toxicity of bioavailable linear alkylbenzene sulfonate in soil. Appl Environ Microbiol 68:3502–3508
Brazil GM, Kenefick L, Callanan M, Haro A, DeLorenzo V, Dowling DN, O’Gara F (1995) Construction of a rhizosphere pseudomonad with potential to degrade polychlorinated biphenyls and detection of bph gene expression in the rhizosphere. Appl Environ Microbiol 61:1946–1952
Bulich AA, Isenberg DL (1981) Use of the luminescent bacterial system for the rapid assessment of aquatic toxicity. ISA Trans 20:29–33
Burlage RS, Sayler GS, Larimer F (1990) Monitoring of naphthalene catabolism by bioluminescence with nah-lux transcriptional fusions. J Bacteriol 172:4749–4757
Burlage RS, Palumbo AV, Heitzer A, Sayler G (1994) Bioluminescent reporter bacteria detect contaminants in soil samples. Appl Biochem Biotechnol 45/46:731–740
Casavant NC, Thompson D, Beattie GA, Phillips GJ, Halverson LJ (2003) Use of a site-specific recombination-based biosensor for detecting bioavailable toluene and related compounds on roots. Environ Microbiol 5:238–249
Dorn JG, Frye RJ, Maier RM (2003) Effect of temperature, pH and initial cell number on luxCDABE and nah gene expression during naphthalene and salicylate catabolism in the reporter bacteriumPseudomonas putida RB1353. Appl Environ Microbiol 69:2209–2216
Elasri MO, Miller RV (1998) A Pseudomonas aeruginosa biosensor responds to exposure to ultraviolet radiation. Appl Microbiol Biotechnol 50:455–458
Elasri M, Delorme S, Lemanceau P, Stewart G, Laue B, Glickmann E, Oger PM, Dessaux Y (2001) Acyl-homoserine lactone production is more common among plant-associated Pseudomonas spp. than among soilborne Pseudomonas spp. Appl Environ Microbiol 67:1198–1209
Elvang AM, Westerberg K, Jernberg C, Jansson JK (2001) Use of green fluorescent protein and luciferase biomarkers to monitor survival and activity of Arthrobacter chlorophenolicus A6 cells during degradation of 4-chlorophenol in soil. Environ Microbiol 3:32–42
Espinosa-Urgel M, Ramos JL (2001) Expression of a Pseudomonas putida involved in lysine metabolism is induced in the rhizosphere. Appl Environ Microbiol 67:5219–5224
Fernandez S, Shingler V, DeLorenzo V(1994) Cross-regulation byXylR and Dmp R activators of Pseudomonas putida suggests that transcriptional control of biodegradative operons evolves independently of catabolic genes. J Bacteriol 176:5052–5058
Füchslin HP, Rüegg I, Van der Meer JR, Egli T (2003) Effect of integration of a GFP reporter gene on fitness of Ralstonia eutropha during growth with 2,4-dichlorophenoxyacetic acid. Environ Microbiol 5:878–887
Ghiglione J-L, Gourbiere F, Potier P, Phillippot L, Lensi R (2000) Role of respiratory nitrate reductase in ability of Pseudomonas fluorescens YT101 to colonize the rhizosphere of maize. Appl Environ Microbiol 66:4012–4016
Hansen LH, Sørensen SJ (2001) The use of whole-cell biosensors to detect and quantify compounds or conditions affecting biological systems. Microb Ecol 42:483–494
Hay AG, Rice JF, Applegate BM, Bright NG, Sayler GS (2000) A bioluminescent whole-cell reporter for detection of 2,4-dichlorophenoxyacetic acid and 2,4-dichlorophenol in soil. Appl Environ Microbiol 66:4589–4594
Heitzer A, Webb OF, Thonnard JE, Sayler GS (1992) Specific and quantitative assessment of naphthalene and salicylate bioavailability by using a bioluminescent catabolic reporter bacterium. Appl Environ Microbiol 58:1839–1846
Heitzer A, Malachowsky K, Thonnard JE, Bienkowski PR, White D, Sayler GS (1994) Optical biosensor for environmental on-line monitoring of naphthalene and salicylate bioavailability with an immobilized bioluminescent catabolic reporter bacterium. Appl Environ Microbiol 60:1487–1494
Heitzer A, Applegate B, Kehrmayer S, Pinkart H, Webb OF, Phelps TJ, White DC, Sayler GS (1998) Physiological considerations of lux reporter fusions. J Microbiol Methods 33:45–57
Højberg O, Schnider U, Winteler HV, Sørensen J, Haas D (1999) Oxygen-sensing reporter strainof Pseudomonasfluorescens for monitoring the distribution of low-oxygen habitats in soil. Appl Environ Microbiol 65:4085–4093
Jaeger CH, Lindow SE, Miller W, Clark W, Firestone MK (1999) Mapping of sugar and amino acid availability in soil around roots with bacterial sensors of sucrose and tryptophan. Appl Environ Microbiol 65:2685–2690
Jansson JK (2003) Marker and reporter genes: illuminating tools for environmental microbiologists. Curr Opin Microbiol 6:310–316
Jensen LE, Nybroe O (1999) Nitrogen availability to Pseudomonas fluorescens DF57 is limited during decomposition of barley straw in bulk soil and in the barley rhizosphere. Appl Environ Microbiol 65:4320–4328
Keane A, Phoenix P, Ghoshal S, Lau PCK (2002) Exposing culprit organic pollutants: a review. J Microbiol Methods 49:103–119
King JMH, DiGrazia PM, Applegate BM, Burlage R, Sanseverino J, Dunbar P, Larimer F, Sayler GS (1990) Rapid, sensitive bioluminescent reporter technology for naphthalene exposure and biodegradation. Science 249:778–781
Koch B, Worm J, Jensen LE, Højberg O, Nybroe O (2001) Carbon limitation induces σS-dependent gene expression in Pseudomonas fluorescens in soil. Appl Environ Microbiol 67:3363–3370
Köhler S, Belkin S, Schmid RD (2000) Reporter gene bioassays in environmental analysis. Fresenius J Anal Chem 366:769–779
Kragelund L, Hosbond C, Nybroe O (1997) Distribution of metabolic activity and phosphate starvation response of lux-tagged Pseudomonas fluorescens reporter bacteria in the barley rhizosphere. Appl Environ Microbiol 63:4920–4928
Kuiper I, Bloemberg GV, Noreen S, Thomas-Oates JE, Lugtenberg BJJ (2001) Increased uptake of putrescine in the rhizosphere inhibits competitive root colonization by Pseudomonas fluorescens strain WCS365. Mol Plant-Microbe Interact 14:1096–1104
Layton AC, Muccini M, Ghosh MM, Sayler GS (1998) Construction of a bioluminescent reporter strain to detect polychlorinated biphenyls. Appl Environ Microbiol 64:5023–5026
Layton AC, Gregory B, Schultz TW, Sayler GS (1999) Validation of genetically engineered surfactant resistant bacteria as toxicity assessment tools. Ecotoxicol Environ Safety 43:222–228
LeBlond JD, Applegate BM, Menn FM, Schultz TW, Sayler GS (2000) Structure-toxicity assessment of metabolites of the aerobic bacterial transformation of substituted naphthalenes. Environ Toxicol Chem 19:1235–1246
Lee SW, Cooksey DA (2000) Genes expressed in Pseudomonas putida during colonization of a plant-pathogenic fungus. Appl Environ Microbiol 66:2764–2772
Leveau JHJ, Lindow SE (2001) Predictive and interpretive simulation of green fluorescent protein expression in reporter bacteria. J Bacteriol 183:6752–6762
Leveau JHJ, Lindow SE (2002) Bioreporters in microbial ecology. Curr Opin Microbiol 5:259–265
Marco ML, Legac J, Lindow SE (2003) Conditional survival as a selection strategy to identify plant-inducible genes of Pseudomonas syringae. Appl Environ Microbiol 69:5793–5801
Marschner P, Crowley DE (1996) Physiological activity of a bioluminescent Pseudomonas fluorescens (strain 2–79) in the rhizosphere of mycorrhizal and non-mycorrhizal pepper (Capsicum annuum L.). Soil Biol Biochem 28:869–876
Meikle A, Amin-Hanjani S, Glover LA, Killham K, Prosser JI (1995) Matric potential and the survival and activity of a Pseudomonas fluorescens inoculum in soil. Soil Biol Biochem 27:881–892
Neilson JW, Pierce SA, Maier RM (1999) Factors influencing expression of luxCDABE and nah genes in Pseudomonas putida RB1353 (NAH7, pUTK9) in dynamic systems. Appl Environ Microbiol 65:3473–3482
Palmer G, McFadzean R, Killham K, Sinclair A, Paton GI (1998) Use of lux-based biosensors for rapid diagnosis of pollutants in arable soils. Chemosphere 36:2683–2697
Park SH, Lee DH, Oh KH, Lee K, Kim CK (2002) Detection of aromatic pollutants by bacterial biosensors bearing gene fusions constructed with the dnaK promoter of Pseudomonas sp DJ-12. J Microbiol Biotechnol 12:417–422
Porteous F, Killham K, Meharg A (2000) Use of a lux-marked rhizobacteriumas a biosensor to assess changes in rhizosphere C flow due to pollutant stress. Chemosphere 41:1549–1554
Rabbow E, Rettberg P, Baumstark C, Horneck G (2002) SOS-LUX and LAC-FLUORO-TEST for the quantification of genotoxic and/or cytotoxic effects of heavy metal salts. Anal Chim Acta 456:31–39
Rainey PB (1999) Adaptation of Pseudomonas fluorescens to the plant rhizosphere. Environ Microbiol 1:243–257
Ramos C, Mølbak L, Molin S (2000) Bacterial activity in the rhizosphere analysed at the single-cell level by monitoring ribosome contents and synthesis rates. Appl Environ Microbiol 66:801–809
Rosen R, Davidov Y, LaRossa RA, Belkin S (2000) Microbial sensors of ultraviolet radiation based on recA’::lux fusions. Appl Biochem Biotechnol 89:151–160
Sanseverino J, Applegate BM, King JMH, Sayler GS (1993) Plasmid-mediated mineralization of naphthalene, phenanthrene, and anthracene. Appl Environ Microbiol 59:1931–1937
Shingler V, Moore T (1994) Sensing of aromatic compounds by the DmpR transcriptional activator of phenol-catabolizing Pseudomonas sp. strain CF600. J Bacteriol 176:1555–1560
Shingler V, Bartilson M, Moore T (1993) Cloning and nucleotide sequence of the gene encoding the positive regulator (DmpR) of the phenol catabolic pathway encoded by pV1150 and identification of DmpR as a member of the NtrC family of transcriptional activators. J Bacteriol 175:1596–1604
Smith LM, Tola E, deBoer P, O’Gara F (1999) Signalling by the fungus Pythium ultimum represses expression of two ribosomal RNA operons with key roles in the rhizosphere ecology of Pseudomonas fluorescens F113. Environ Microbiol 1:495–502
Standing D, Meharg AA, Killham K (2003) A tripartite microbial reporter gene system for real-time assays of soil nutrient status. FEMS Microbiol Lett 220:35–39
Stiner L, Halvorsen LJ (2002) Development and characterization of a green fluorescent protein-based bacterial biosensor for bioavailable toluene and related compounds. Appl Environ Microbiol 68:1962–1971
Sørensen J (1997) The rhizosphere as a habitat for soil microorganisms. In: Van Elsas JD, Trevors JT, Wellington EMH (eds) Modern soil microbiology. Marcel Dekker, Inc., New York, pp 21–45
Sørensen J, Jensen LE, Nybroe O (2001) Soil and rhizosphere as habitats for Pseudomonas inoculants: new knowledge on distribution, activity and physiological state derived from micro-scale and single-cell studies. Plant Soil 232:97–108
Timms-Wilson TM, Ellis RJ, Bailey MJ (2000) Immuno-capture differential display method (IDDM) for the detection of environmentally induced promoters in rhizobacteria. J Microbiol Methods 41:77–84
Tsien RY (1998) The green fluorescent protein. Annu Rev Biochem 67:509–544
Unge A, Tombolini R, Mølbak L, Jansson JK (1999) Simultaneous monitoring of cell number and metabolic activity of specific bacterial populations with a dual gfp-luxAB marker system. Appl Environ Microbiol 65:813–821
Van der Meer JR, Trope D, Jaspers M (2004) Illuminating the detection chain of bacterial bioreporters. Environ Microbiol 6:1005–1020
Van Dyk TK, Majarian WR, Konstantinov KB, Young RM, Dhurjati PS, LaRossa RA (1994) Rapid and sensitive pollutant detection by induction of heat-shock genebioluminescence gene fusions. Appl Environ Microbiol 60:1414–1420
Van Dyk TK, Smulski DR, Reed TR, Belkin S, Vollmer AC, LaRossa R (1995) Responses to toxicants of an Escherichia coli strain carrying a uspA’::lux genetic fusion and an E. coli strain carrying a grpE’::lux fusion are similar. Appl Environ Microbiol 61:4124–4127
Van Overbeek LS, Van Elsas JD, Van Veen JA (1997) Pseudomonas fluorescens Tn5-B20 mutant RA92 responds to carbon limitation in soil. FEMS Microbiol Ecol 24:57–71
Vollmer AC, Belkin S, Smulski DR, Van Dyk TK, LaRossa RA (1997) Detection of DNA damage by use of Escherichia coli carrying recA’::lux, uvrA’::lux, or alkA’::lux reporter plasmids. Appl Environ Microbiol 63:2566–2571
Weitz HJ, Ritchie JM, Bailey DA, Horsburgh AM, Killham K, Glover LA (2001) Construction of a modified mini-Tn5 luxCDABE transposon for the development of bacterial biosensors for ecotoxicity testing. FEMS Microbiol Lett 197:159–165
Willardson BM, Wilkins JF, Rand TA, Schupp JM, Hil KK, Keim P, Jackson PJ (1998) Development and testing of a bacterial biosensor for toluene-based environmental contaminants. Appl Environ Microbiol 64:1006–1012
Wise AA, Kuske CR (2000) Generation of novel regulatory proteins that detect priority pollutant phenols. Appl Environ Microbiol 66:163–169
Yeomans C, Porteous F, Paterson E, Meharg AA, Killham K (1999) Assessment of lux-marked Pseudomonas fluorescens for reporting on organic carbon compounds. FEMS Microbiol Lett 176:79–83
Yolcubal I, Piatt JJ, Pierce SA, Brusseau ML, Maier RM (2000) Fiber optic detection of in situ lux reporter gene activity in porous media: system design and performance. Anal Chim Acta 422:121–130
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Sørensen, J., Nybroe, O. (2006). Reporter Genes in Bacterial Inoculants Can Monitor Life Conditions and Functions in Soil. In: Nannipieri, P., Smalla, K. (eds) Nucleic Acids and Proteins in Soil. Soil Biology, vol 8. Springer, Berlin, Heidelberg . https://doi.org/10.1007/3-540-29449-X_16
Download citation
DOI: https://doi.org/10.1007/3-540-29449-X_16
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-29448-1
Online ISBN: 978-3-540-29449-8
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)