Skip to main content
SpringerLink
Log in

Bioluminescent reporter bacteria detect contaminants in soil samples

  • Session 5 Environmental Biotechnology
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Reporter strains of bacteria were tested using soil samples from several sites near a leaking fuel oil storage facility. The reporter bacteria utilized the bioluminescentlux genes fromVibrio fischeri, which were transcriptionally fused to catabolic gene sequences. The catabolic genes of interest specified the degradation of toluene (from the TOL plasmid) and naphthalene (from the NAH7 plasmid and from a NAH plasmid recently isolated). The results indicated that two soil samples were contaminated with both toluene (or xylene) and naphthalene. These data were useful in describing the extent of contamination at the site.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Battelle Second International Symposium, In Situ and On-site Bioreclamation (abstracts). San Diego, CA. April, 1993.

  2. Meighen, E. A. (1988),Ann. Rev. Microbiol. 42, 151–176.

    Article  CAS  Google Scholar 

  3. Rogowsky, P. M., Close, T. J., Chimera, J. A., Shaw, J.J., and Kado, C. L. (1987),J. Bacteriol. 169, 5101–5112.

    CAS  Google Scholar 

  4. King, J. M. H., DiGrazia, P. M., Applegate, B., Burlage, R., Sanseverino, J., Dunbar, P., Larimer, F., and Sayler, G. S. (1990),Science 249, 778–781.

    Article  CAS  Google Scholar 

  5. Burlage, R. S., Sayler, G. S., and Larimer, F. (1990),J. Bacteriol. 172, 4749–4757.

    CAS  Google Scholar 

  6. Burlage, R. S., Palumbo, A. V., and Sayler, G. S. Toluene and m-methyl benzyl alcohol show different patterns of induction of thexyl upper pathway ofPseudomonas putida mt-2. Manuscript in preparation.

  7. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989),Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  8. Heitzer, A., Webb, O. F., Thonnard, J. E., and Sayler, G. S. (1992),Appl. Environ. Microbiol. 58, 1839–1846.

    CAS  Google Scholar 

  9. Assinder, S. J. and Williams, P. A. (1990),Adv. Microb. Physiol. 31, 1–69.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN

    Robert S. Burlage & Anthony V. Palumbo

  2. Center for Environmental Biotechnology, University of Tennessee, Knoxuille, TN

    Armin Heitzer & Gary Sayler

Authors
  1. Robert S. Burlage
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anthony V. Palumbo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Armin Heitzer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gary Sayler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Burlage, R.S., Palumbo, A.V., Heitzer, A. et al. Bioluminescent reporter bacteria detect contaminants in soil samples. Appl Biochem Biotechnol 45, 731–740 (1994). https://doi.org/10.1007/BF02941845

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02941845

Index Entries

Search

Navigation