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STR Typing and Available Kits

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DNA Fingerprinting: Advancements and Future Endeavors

Abstract

This chapter describes a time-scale development of short tandem repeats (STRs) and STR-based DNA technology used in forensic DNA analysis. The text describes the subsequent advancements in the development of the STR multiplex systems. This sequential development in STR-based multiplex systems has increased efficiency, sensitivity, and inhibitor tolerance with improved buffers. The development of different variants of STR-based multiplex systems utilizing different variants including autosomal, mini-, Y-, and X-STRs has increased the flexibility and ease of the forensic DNA analyst. With the availability of genetic analyzers, utilizing sixth dye has created new possibilities toward availability of expanded STR multiplex without affecting the requirement of input DNA for PCR. Rapid PCR and application of next-generation sequencing are also discussed along with the already validated capillary electrophoresis technology.

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Author information

Authors and Affiliations

  1. DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar, Madhya Pradesh, India

    Pankaj Shrivastava & Hirak Ranjan Dash

  2. State Forensic Science Laboratory, Jaipur, Rajasthan, India

    R. K. Kumawat

  3. Dr. A.P.J. Abdul Kalam Institute of Forensic Science, Bundelkhand University, Jhansi, Uttar Pradesh, India

    Ankit Srivastava

  4. DNA Fingerprinting Unit, State Forensic Science Laboratory, Department of Home, Jail and Disaster Management, Government of Jharkhand, Ranchi, India

    Jahangir Imam

Authors
  1. Pankaj Shrivastava
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  2. Hirak Ranjan Dash
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  3. R. K. Kumawat
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  4. Ankit Srivastava
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  5. Jahangir Imam
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Editor information

Editors and Affiliations

  1. DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar, Madhya Pradesh, India

    Hirak Ranjan Dash

  2. DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar, Madhya Pradesh, India

    Pankaj Shrivastava

  3. Department of Biology and DNA Fingerprinting Unit, Central Forensic Science Laboratory, New Delhi, Delhi, India

    Braja Kishore Mohapatra

  4. Department of Life Science, National Institute of Technology, Rourkela, Odisha, India

    Surajit Das

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Shrivastava, P., Dash, H.R., Kumawat, R.K., Srivastava, A., Imam, J. (2018). STR Typing and Available Kits. In: Dash, H., Shrivastava, P., Mohapatra, B., Das, S. (eds) DNA Fingerprinting: Advancements and Future Endeavors. Springer, Singapore. https://doi.org/10.1007/978-981-13-1583-1_4

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