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Novel Intermolecular Surface Force Unveils the Driving Force of the Actomyosin System

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The Role of Water in ATP Hydrolysis Energy Transduction by Protein Machinery

Abstract

In this chapter, we discuss the role of water in actomyosin-force generation. We have been investigating the hydration properties of ions, organic molecules, and proteins. These studies revealed that actin filaments (F-actin) are surrounded by a hyper-mobile water (HMW) layer and restrained water layer, while myosin subfragment 1 (S1) has only a typical restrained hydration layer. The understanding of the physicochemical properties of HMW has been greatly advanced by recent theoretical studies on statistical mechanics and solution chemistry. To explain the mechanism of force generation of actomyosin using ATP hydrolysis, we propose a driving force hypothesis based on novel intermolecular surface force. This hypothesis is consistent with the reported biochemical kinetics and thermodynamic parameters for the primary reaction steps. The gradient field of solvation free energy of S1 is generated in close proximity to F-actin.

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Authors and Affiliations

  1. Biological and Molecular Dynamics, Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai, 980-8577, Japan

    Makoto Suzuki

  2. Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, 6-6-07 Aoba, Aramaki, Aoba-ku, Sendai, 980-8579, Japan

    Makoto Suzuki

  3. Department of Materials Processing, Graduate School of Engineering, Tohoku University, 6-6-02 Aoba, Aramaki, Aoba-Ku, Sendai, 980-8579, Japan

    George Mogami & Takahiro Watanabe

  4. Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, 560-8531, Japan

    Nobuyuki Matubayasi

  5. Elements Strategy Initiative for Catalysts and Batteries, Kyoto University, Katsura, Kyoto, 615-8520, Japan

    Nobuyuki Matubayasi

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  1. Makoto Suzuki
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  2. George Mogami
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  3. Takahiro Watanabe
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  4. Nobuyuki Matubayasi
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Correspondence to Makoto Suzuki .

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  1. Institute of Engineering Education, Tohoku University, Sendai, Miyagi, Japan

    Makoto Suzuki

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Suzuki, M., Mogami, G., Watanabe, T., Matubayasi, N. (2018). Novel Intermolecular Surface Force Unveils the Driving Force of the Actomyosin System. In: Suzuki, M. (eds) The Role of Water in ATP Hydrolysis Energy Transduction by Protein Machinery. Springer, Singapore. https://doi.org/10.1007/978-981-10-8459-1_16

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