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Nanoplasmonic and Microfluidic Devices for Biological Sensing

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Nano-Optics: Principles Enabling Basic Research and Applications

Abstract

In this chapter we report about recent advances on the development and application of 2D and 3D plasmonic nanostructures used for sensing of biological samples by Raman spectroscopy at unprecedented resolution of analysis. Besides, we explain how the integration of these nanodevices in a microfluidic apparatus can simplify the analysis of biological samples. In the first part we introduce and motivate the convenience of using nanoplasmonic enhancers and Raman spectroscopy for biological sensing, describing the phenomena and the current approaches to fabricate nanoplasmonic structures. In the second part, we explain how specific multi-element devices produce the optimal enhancement of the Raman scattering. We report cases where biological sensing of DNA was performed at few molecules level with nanometer spatial resolutions. Finally, we show an example of microfluidic device integrating plasmonic nanodevices to sort and drive biological samples, like living cells, towards the optical probe in order to obtain optimal conditions of analysis.

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Acknowledgments

We acknowledge all contributors that contributed in this work from other institution, in particular the researchers from the previous Nanostructure Division at IIT (Italian Institute of Technology) in Genoa.

We thank for the financial support the King Abdullah University of Science and Technology start-up fund and the Italian Minister of Health (projects nos. GR-2010-2320665 and GR-2010-2311677)

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Authors and Affiliations

  1. Bionem Lab, Departement of Experimental Clinics, Campus “Salvatore Venuta”, University Magna Graecia, Viale Europa, 88100 Germaneto-Catanzaro, Italy

    G. Perozziello, M. L. Coluccio & P. Candeloro

  2. Physical Science and Engineering Division (PSE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia

    A. Giugni, M. Allione, B. Torre, G. Das, M. Marini, L. Tirinato, M. Moretti, T. Limongi & Enzo Di Fabrizio

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  1. G. Perozziello
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  2. A. Giugni
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  3. M. Allione
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  4. B. Torre
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  5. G. Das
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  6. M. L. Coluccio
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  7. M. Marini
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  9. M. Moretti
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  10. T. Limongi
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  11. P. Candeloro
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  12. Enzo Di Fabrizio
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Corresponding author

Correspondence to Enzo Di Fabrizio .

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Editors and Affiliations

  1. Department of Physics, Boston College, Massachusetts, USA

    Baldassare Di Bartolo

  2. Department of Physics and Astronomy, Wheaton College, Norton, Massachusetts, USA

    John Collins

  3. Department of Physics, Boston College, Massachusetts, USA

    Luciano Silvestri

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Perozziello, G. et al. (2017). Nanoplasmonic and Microfluidic Devices for Biological Sensing. In: Di Bartolo, B., Collins, J., Silvestri, L. (eds) Nano-Optics: Principles Enabling Basic Research and Applications. NATO Science for Peace and Security Series B: Physics and Biophysics. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-0850-8_12

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  • DOI: https://doi.org/10.1007/978-94-024-0850-8_12

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