Abstract
During the last decade, the isolation and culture of protoplasts has been described for grain legumes of the genera Cajanus,Pisum and Vigna, while in forage legumes, protoplasts of Astragalus, Medicago, Onobrychis and Trifolium have received most attention. Reports on the transformation of legume protoplasts are limited. Somatic hybridisation has been directed towards the grain legumes Lathryus, Pisum and Vigna, with Lotus,Medicago, Onobrychis and Trifolium being targeted in forage crops. Several reports have described the use of legume protoplasts as experimental material in physiological investigations and in studies of plant-pathogen interactions.
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Davey, M.R., Marchant, R., Power, J.B. (2003). Protoplasts of Grain and Forage Legumes: Their Exploitation in Genetic Manipulation, Physiological Investigations and Plant-Pathogen Interactions. In: Jaiwal, P.K., Singh, R.P. (eds) Improvement Strategies of Leguminosae Biotechnology. Focus on Biotechnology, vol 10A. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0109-9_5
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DOI: https://doi.org/10.1007/978-94-017-0109-9_5
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